Chromatography Knowledge Center
Explore Chromatography Guides, Compatibility Tools, and Technical Insights
Browse educational resources covering HPLC, GC, LC/MS, autosampler vials, fittings, and sample preparation workflows
The Chrom Tech Knowledge Center is a practical reference library for chromatography laboratories—built to help you select the right consumables, understand instrument compatibility, and troubleshoot analytical workflows across LC, GC, and sample preparation.
Use the topic sections below to explore technical guidance for autosampler vials, microplates, chromatography columns, sample preparation, and instrument accessories.
- Instrument compatibility
- Vial selection
- Closure selection
- Septa selection
- Plate uses
- Selection guidance
- Glass vs polypropylene
- Sealing methods
- Column care
- Peak resolution
- Guard vs inline filters
- Poroshell methods
- Fittings and unions
- Tubing selection
- Leak troubleshooting
- Dead volume and flow path
- Pump troubleshooting
- Dual piston advantages
- Pulse dampeners
- Pump maintenance
- Column selection
- Dimensions and film thickness
- Stationary phase choice
- Method fit
- Sample prep efficiency
- Workflow streamlining
- Bench top instrumentation
- Laboratory process improvement
- Syringe selection
- GC and HPLC use
- Cleaning and handling
- Care best practices
- Gas purification
- Flow measurement
- Leak checking
- GC system protection
- Model-specific supplies
- Autosampler parts
- Inert flow path solutions
- GC support resources
- Syringe filter selection
- Membrane compatibility
- Glass prefilters
- Filter vials vs syringe filters
- EHS compliance
- Waste containment systems
- Safe solvent handling
- HPLC waste disposal
Autosampler Vials
Use these guides to match vial dimensions, thread types, and closure systems to your autosampler platform and method needs.
- How to Choose Autosampler Vials for HPLC & GC
- What’s the Difference Between a 1.5 mL and 2.0 mL Autosampler Vial?
- Glass, Plastic & Amber Vials: Which Is Best?
- How to Choose the Right Autosampler Vial Closure for Chromatography
- When Should You Use Vial Inserts in Autosampler Vials?
- How to Choose Storage Vials: Threading, F217 Liners & Seal Integrity
- Autosampler Vial Septa: How to Choose for Solvent Compatibility
- When to Use High Recovery Vials in Chromatography
- Prevent Sample Adsorption: Choosing Silanized or Premium Glass Vials
- Autosampler Vial Compatibility Guide – Waters, Agilent, Shimadzu, Thermo
- What Vials are Compatible with Agilent Technologies GC Autosamplers
- What Vials are Compatible with Agilent Technologies LC Autosamplers
- What Vials are Compatible with Thermo LC Autosamplers
- What Vials are Compatible with Waters Corporation Autosamplers
96-Well Plates
Explore practical guidance on 96-well plate formats, plate materials, well geometry, sealing methods, automation compatibility, and LC-MS sample preparation workflows.
- What Are 96-Well Plates Used For in LC-MS and Sample Prep?
- 96-Well Plate Selection Guide for LC-MS Workflows
- 96-Well Plate Bottom Types: Flat, U, V, and Pyramid
- Vials vs 96-Well Plates for LC-MS Sample Preparation
- Polypropylene vs Glass 96-Well Plates Explained
- Why Use 96-Well Plates with Glass Inserts for LCMS and Chromatography
- How to Maximize Sample Recovery in 96-Well Plates
- 96-Well Plate Automation Guide
- 96-Well vs 384-Well Plates: Key Differences Explained
- 96-Well Plate Sealing Methods: Films, Foils, and Cap Mats
- 96-Well Plate Dimensions: ANSI/SBS Standards Explained
LC Columns
Explore practical guidance on HPLC and UHPLC column care, pressure control, particle and pore size selection, guard column use, and Poroshell method optimization.
- HPLC Column Care and Maintenance Guide
- How Many Injections Can an HPLC Column Last?
- How to Extend HPLC Column Lifetime
- How Column Temperature Affects HPLC Resolution
- HPLC Column Pore Size vs Particle Size Explained
- How to Improve HPLC Peak Resolution
- How to Regenerate a C18 HPLC Column
- What Is End-Capping in HPLC Columns?
- What Is a Guard Column in HPLC?
- Inline Filter vs Guard Column for HPLC
- How to Reduce HPLC Column Backpressure
- Optimizing HPLC Methods with Poroshell Columns
- Method Transfer Between Poroshell and ZORBAX HPLC Columns
LC Accessories
Explore practical guidance on HPLC fittings, tubing, filters, valves, dead volume, backpressure, leaks, and full flow path optimization for LC and UHPLC systems.
HPLC Pumps
Explore practical guidance on HPLC pump care, troubleshooting, dual piston performance, pulse dampening, priming, piston seal replacement, and pump selection for analytical, preparative, and continuous-flow applications.
- HPLC Pump Care and Troubleshooting Guide
- Advantages of Dual Piston HPLC Pumps
- Choosing a Pump for Continuous Process Applications
- 4 Questions to Ask Before Buying an HPLC Pump
- How Pulse Dampeners Work in HPLC Pumps
- Choosing Pumps for High-Pressure Solvent Applications
- When to Change an HPLC Piston Seal
- How to Prime an HPLC Pump
GC Columns
Explore practical guidance on choosing GC columns by application, understanding column dimensions and film thickness, and selecting the right stationary phase for method performance and separation goals.
Bench Top Instrumentation
Explore practical guidance on sample preparation instrumentation, laboratory workflow efficiency, and selecting bench top tools that support more consistent day-to-day sample handling and preparation.
Manual Syringes
Explore practical guidance on selecting manual syringes for GC, HPLC, and laboratory applications, along with care, cleaning, handling, and best practices for longer syringe life and more reliable use.
Gas Management
Explore practical guidance on GC gas purification, measuring gas flow, and checking for leaks to help protect columns and detectors, support stable gas delivery, and improve overall gas chromatography reliability.
Agilent GC Replacement Parts
Explore model-specific resources for Agilent GC replacement parts, autosampler supplies, inert flow path components, and supporting guides that help maintain reliable gas chromatography system performance.
- Agilent 7890B GC Supplies
- Agilent 7890A & 6890 GC Supplies
- Agilent 6850 Gas Chromatograph Supplies
- Agilent Supplies for HP 5890 GC
- Agilent GC Autosampler Supplies (7673, G1513A, 7683)
- Agilent 7694 Headspace Sampler Supplies
- Agilent 7010B & 7000D Triple Quadrupole GC/MS Supplies
- Agilent Inert Flow Path Solutions
- Gas Chromatography Vials: How to Choose the Right Vial
- How to Measure Gas Flow in Gas Chromatography (GC) Systems
Filtration Supplies
Explore practical guidance on syringe filtration, membrane compatibility, glass prefilters, and selecting the right filtration approach for cleaner, more reliable sample preparation workflows.
Solvent Containment
Explore practical guidance on HPLC solvent waste management, compliant waste containment systems, safe disposal practices, and support resources for cleaner, safer laboratory solvent handling.