Blog

10th Jun 2024

Five Ways to Reduce Costs in Your Chromatography Workflow

How You Can Save Money in Your Chromatography Laboratory Chromatography is a powerful analytical technique, but costs can add up quickly. Lab managers are regularly challenged to keep costs to a min …

read more

31st May 2024

How to Cut HPLC Tubing: Your Guide to Peak Performance

High-pressure liquid chromatography (HPLC) is a powerful analytical technique, but results can depend greatly on seemingly minor details, such as the tubing used throughout the system. In this arti …

read more

16th May 2024

Pore Size vs. Particle Size in HPLC Columns

When selecting an  HPLC column, it is important to understand the different attributes and how they will affect your chromatography. Chemical properties, such as the type of surface of the s …

read more

15th May 2024

Optimizing HPLC Methods with Agilent’s InfinityLab Poroshell 120 Columns

More Chemistries, More Choices for Separation The InfinityLab Poroshell 120 family has grown to include three particle sizes and 20 chemistries, so you can efficiently separate the widest variety …

read more

9th Apr 2024

How to Choose the Best Storage Vials

Solving Storage Challenges with F217 Liners Screw thread vials are common for LC sample analysis as well as sample or standard storage. But is there a difference between autosampler vials and stor …

read more

27th Mar 2024

Are Your Autosampler Vials Compromising Your Results?

Exploring the Impact of Vial Quality on HPLC and GC Performance In the world of chromatography, choosing the appropriate instrumentation and accessories is essential for achieving accurate and rel …

read more

12th Mar 2024

What are the Advantages of Dual Piston Pumps?

Chrom Tech HPLC pumps are used for many applications, many of which are not even chromatography-related. Pump specifications can dramatically impact the precision, reliability, and efficiency of …

read more

11th Mar 2024

5 Features to Consider When Choosing a Pump for Continuous Process Applications

Replacing Pharma's Conventional Batch Processing In diverse manufacturing sectors, transitioning from conventional batch chemistry to continuous process applications prior to scaling up holds th …

read more

5th Jan 2024

Revolutionizing Your Chromatography | The Power of Poroshell and more

Are you frustrated with increasing backpressures from your current HPLC column? Do you wish you could achieve UHPLC results with HPLC instrumentation? Choosing the proper tools for success is importan …

read more

3rd Jan 2024

Unlocking the Benefits of Agilent Bond Elut HLB SPE

Discover how Agilent Bond Elut HLB SPE products can revolutionize your sample preparation process and provide optimal results.Understanding the Importance of Sample Preparation Sample prepar …

read more

30th Nov 2023

Revolutionizing UHPLC Connections: Introducing MarvelXACT by IDEX

Reliable HPLC fitting connections play a crucial role in ensuring accurate and consistent results in UHPLC (Ultra-High Performance Liquid Chromatography) applications. Improper fittings can l …

read more

7th Nov 2023

EHS Compliance: Safely Manage HPLC Solvent Waste

Many of the solvents, and even samples, used in high performance liquid chromatography (HPLC) are hazardous and/or flammable. Because of this, it’s important to mitigate health and safety risks t …

read more

11th Oct 2023

The 3 Most Common Applications for Headspace Vials

Gas chromatography (GC) is a widely-used technique for separating volatile compounds in mixtures. One specific method, headspace GC, is employed to extract and analyze limited volatile components …

read more

15th Sep 2023

How Does Column Temperature Affect HPLC Resolution?

HPLC Column temperature affects retention time, selectivity, peak shape, and pressure. Let’s look at this in more detail.Temperature Can Affect Retention Time Retention measures the time it ta …

read more

15th Aug 2023

How to Save Money and Increase Accuracy on Sample Prep

Laboratories can instantly increase throughput and improve their sample preparation accuracy with automation, but generally automation is only common in high throughput laboratories. But, what if th …

read more

7th Jul 2023

Syringe Filters vs Filter Vials: What is the Best Method for Filtration?

For most chromatography applications, a sample taken directly from the source is usually not in a form that can be directly placed into the analytical instrument. Sample preparation can vary vastly, f …

read more

13th Jun 2023

How To Choose the Right Vial for Chromatography

In general, modern chromatography liquid autosamplers accept 12 x 32mm vials, so we will assume that a 12 x 32mm vial is compatible with your autosampler (Headspace autosamplers accept 20mm wide autos …

read more

8th May 2023

HPLC Pump Care and Troubleshooting

Many of the technical support inquiries we receive at Chrom Tech are related to care and troubleshooting of our continuous process pumps. Some of the topics include:       …

read more

13th Apr 2023

The Benefits of Glass Well Plate Inserts

96 well plates are a standard consumable in high-throughput analytical laboratories that utilize mass spectrometry. Most commonly, we see polypropylene 96 deep well plates 2mL square or 1mL round 9 …

read more

9th Mar 2023

Syringe Filters for HPLC Sample Preparation: A Guide to Choosing the Right Filter for Consistent and Accurate Results

Sample preparation is a crucial step in high-performance liquid chromatography (HPLC). While sample preparation can vary depending on the type of application and level of interference a sample contain …

read more

13th Feb 2023

How to Choose the Right Septa for Your Autosampler Vial Closure

When performing LC and GC analysis, you’ll need something to store and transport your samples prior to injection. Chromatography vials are the most common vessel, but come in a variety of shapes and s …

read more

19th Jan 2023

Maximizing Throughput in an HPLC Laboratory

There are many reasons to maximize sample throughput in a laboratory, but generally, cost savings is a huge driving factor. While optimizing the HPLC analysis is very beneficial, it’s important to loo …

read more

15th Nov 2022

What Should Forensic Labs Be Using for Blood Alcohol Testing?

Headspace injection and dual column GC-FID is an extremely common method for detecting blood alcohol concentration (BAC) in forensic labs. Due to the severity of consequences associated with an indivi …

read more

15th Nov 2022

How to Check for Leaks in a GC System

To achieve reliable, reproducible, and accurate results, maintaining a leak-free GC or GC/MS system is critical. Even small leaks in the system can negatively impact the chromatography through loss of …

read more

18th Oct 2022

New and Dangerous Synthetic Opioids

Are New Synthetic Opioids Posing Increased Risks?Synthetic opioids are commonly used in clinical settings as they provide analgesic effects for individuals suffering from severe pain. Unfortunately, t …

read more

11th Oct 2022

Methods for Changing Peak Resolution in HPLC

Improving the resolution of closely eluting sample components is a challenge that all chromatographers will face at one time or another. Several methods for changing peak resolution in HPLC include us …

read more

18th Sep 2022

What is the Smallest Sample Volume You Can Use in 96 Well Plates?

For high throughput laboratories, 96-well plates offer a number of advantages over chromatography vials such as cost savings and easy automation capabilities. Different well plate types serve differen …

read more

15th Sep 2022

What Sample Prep is Best for Vitamin D Analysis?

Low level testing of endogenous vitamin and hormone levels in biological samples is the driving force behind the need for more sensitive and specific analytical methods. One example is vitamin D analy …

read more

16th Aug 2022

Why You Should be Using a Delay Column for PFAS Analysis

PFAS in the Environment TodayPoly- and perfluoroalkyl substances (PFAS) are rapidly emerging as some of the most important environmental contaminants to monitor around the world. Known as “forever che …

read more

15th Aug 2022

Which Type of Glass Vial is Best for your Application?

Glass autosampler vials are commonly used for inertness, but with the many varieties of glass options, how do you decide which type of glass vials are suitable for your application? In this blog, we’l …

read more

12th Jul 2022

How Does ICP-MS Work?

How Does an ICP-MS Work?Inductively coupled plasma mass spectrometry (ICP-MS) is a method used to determine which elements something is made of, as opposed to molecules and compounds that are measured …

read more

12th Jul 2022

Should You Use a Glass Pre-Filter Syringe Filter?

Should You be Using a Glass Pre-filter Syringe Filter?Proper sample filtration improves both the quality and consistency of analytical results, while also prolonging the lifetime of your instrument’s …

read more

2nd Jun 2022

Safety Waste Compliance in One Easy Step

Improper waste collection and handling of hazardous waste can put the people and property in a lab at risk. Long gone are the days of dumping HPLC solvent waste down the drain, but even experienced ch …

read more

2nd Jun 2022

Three Reasons You’ll Want to Switch to Agilent Capillary Tubing

Stainless steel tubing is the most popular material for HPLC and UHPLC due to it being compatible with many HPLC solvents, and able to withstand very high pressures. PEEK is the most common alternativ …

read more

12th May 2022

One Simple Step to Conserve Helium

As most people in the GC industry are well aware, the helium shortage is still in full swing. Just a few weeks back, we posted a blog about alternative carrier gases. But we know that not everyone can …

read more

12th May 2022

Revisiting ‘The Big Pain’

Throughout the pandemic, drug testing facilities were suddenly slammed with COVID-19 testing, meaning other testing (drug screening, etc.) was pushed to the wayside. This meant regular employee drug s …

read more

12th Apr 2022

What LC Column Should I Use for EtG and EtS Detection?

Ethyl glucuronide (EtG) and ethyl sulfate (EtS) biomarkers are formed by the liver after ethanol ingestion. They can be detected in urine approximately 2-80 hours after alcohol is consumed. Due to the …

read more

12th Apr 2022

Using 96 Well Plates In High Throughput Laboratories

Using 96 well plates instead of autosampler vials has significant advantages for high throughput labs, due to the ease of automation. Unfortunately, not all steps in the process can be fully automated …

read more

12th Apr 2022

Do You Really Need Helium as Your Carrier Gas?

Unpredictable helium supply can be problematic for chromatographers. With ongoing helium shortages, some labs are being rationed to 50% of their normal supply. Chrom Tech can help you find an alternat …

read more

10th Mar 2022

When to Use High Recovery Vials

There are two main reasons for labs to utilize high recovery vials. The first, and likely most obvious, is for limited-volume samples. In these cases, getting the last few microliters is crucial for s …

read more

6th Mar 2022

How Many Injections Can I Get on My HPLC Column?

It depends! Many factors affect how many injections you can get on your column, including: Injecting “dirty” samplesFollowing HPLC Column manufacturers recommended protocol/operating conditionsUsing …

read more

11th Feb 2022

How do Stainless-Steel Fittings Differ from Polymer-Based Fittings?

Both stainless-steel and polymer-based fittings are quite common in HPLC, and there are pros and cons for each. Deciding which to use for your lab depends on the application and requirements of your m …

read more

11th Feb 2022

Method Transfer between Poroshell and Zorbax HPLC Columns

One of the primary benefits to using superficially porous particle (SPP) HPLC columns, as opposed to totally porous particles is the results yield the efficiency of sub-2-µm, fully porous columns, wit …

read more

19th Jan 2022

True Crime and Chromatography

People have always been fascinated by mystery, and in recent years there has been a major rise in true crime series: podcasts, tv shows, and even books. The horrible, gruesome tragedies have become a …

read more

19th Jan 2022

Reduce Instrument Downtime with Sample Preparation

Reducing downtime in the laboratory saves time and money spent on getting the instrument back up and running. One easy way to reduce the downtime of your HPLC is to prolong the life of your HPLC colum …

read more

9th Dec 2021

Help! My Chromatography Vials are on Backorder!

As we all know, supply chain issues continue to cause issues in the lab. We’ve noticed an increase in people reaching out to see if we have certain vials in stock because they’re on backorder elsewher …

read more

9th Dec 2021

How to Connect a Syringe to HPLC Tubing

The need to connect a syringe to your LC pump to pull mobile phase through for priming (among other applications) is fairly common. We’ve seen some creative ways that people in the laboratory have con …

read more

15th Nov 2021

ASK BETHANY | Should I Use a Guard Column

HPLC ColumnsOne of the most important components in an LC system is the column, which is where analytes are separated out from a mixture for qualitative and quantitative analysis. HPLC columns need to …

read more