Blog

15th Sep 2023

How Does Column Temperature Affect HPLC Resolution?

HPLC Column temperature affects retention time, selectivity, peak shape, and pressure. Let’s look at this in more detail.Temperature Can Affect Retention Time Retention measures the time it ta …

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15th Aug 2023

How to Save Money and Increase Accuracy on Sample Prep

Laboratories can instantly increase throughput and improve their sample preparation accuracy with automation, but generally automation is only common in high throughput laboratories. But, what if th …

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7th Jul 2023

Syringe Filters vs Filter Vials: What is the Best Method for Filtration?

For most chromatography applications, a sample taken directly from the source is usually not in a form that can be directly placed into the analytical instrument. Sample preparation can vary vastly, f …

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13th Jun 2023

How To Choose the Right Vial for Chromatography

In general, modern chromatography liquid autosamplers accept 12 x 32mm vials, so we will assume that a 12 x 32mm vial is compatible with your autosampler (Headspace autosamplers accept 20mm wide autos …

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8th May 2023

HPLC Pump Care and Troubleshooting

Many of the technical support inquiries we receive at Chrom Tech are related to care and troubleshooting of our continuous process pumps. Some of the topics include:       …

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13th Apr 2023

The Benefits of Glass Well Plate Inserts

96 well plates are a standard consumable in high-throughput analytical laboratories that utilize mass spectrometry. Most commonly, we see polypropylene 96 deep well plates 2mL square or 1mL round 9 …

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9th Mar 2023

Syringe Filters for HPLC Sample Preparation: A Guide to Choosing the Right Filter for Consistent and Accurate Results

Sample preparation is a crucial step in high-performance liquid chromatography (HPLC). While sample preparation can vary depending on the type of application and level of interference a sample contain …

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13th Feb 2023

How to Choose the Right Septa for Your Autosampler Vial Closure

When performing LC and GC analysis, you’ll need something to store and transport your samples prior to injection. Chromatography vials are the most common vessel, but come in a variety of shapes and s …

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19th Jan 2023

Maximizing Throughput in an HPLC Laboratory

There are many reasons to maximize sample throughput in a laboratory, but generally, cost savings is a huge driving factor. While optimizing the HPLC analysis is very beneficial, it’s important to loo …

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14th Dec 2022

What Type of Pump is Best for Continuous Process Applications?

In various manufacturing industries, switching from batch chemistry to continuous process applications before scaling up can significantly reduce waste, minimize environmental impacts, and save time a …

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15th Nov 2022

What Should Forensic Labs Be Using for Blood Alcohol Testing?

Headspace injection and dual column GC-FID is an extremely common method for detecting blood alcohol concentration (BAC) in forensic labs. Due to the severity of consequences associated with an indivi …

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15th Nov 2022

How to Check for Leaks in a GC System

To achieve reliable, reproducible, and accurate results, maintaining a leak-free GC or GC/MS system is critical. Even small leaks in the system can negatively impact the chromatography through loss of …

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18th Oct 2022

New and Dangerous Synthetic Opioids

Are New Synthetic Opioids Posing Increased Risks?Synthetic opioids are commonly used in clinical settings as they provide analgesic effects for individuals suffering from severe pain. Unfortunately, t …

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11th Oct 2022

Methods for Changing Peak Resolution in HPLC

Improving the resolution of closely eluting sample components is a challenge that all chromatographers will face at one time or another. Several methods for changing peak resolution in HPLC include us …

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18th Sep 2022

What is the Smallest Sample Volume You Can Use in 96 Well Plates?

For high throughput laboratories, 96-well plates offer a number of advantages over chromatography vials such as cost savings and easy automation capabilities. Different well plate types serve differen …

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15th Sep 2022

What Sample Prep is Best for Vitamin D Analysis?

Low level testing of endogenous vitamin and hormone levels in biological samples is the driving force behind the need for more sensitive and specific analytical methods. One example is vitamin D analy …

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16th Aug 2022

Why You Should be Using a Delay Column for PFAS Analysis

PFAS in the Environment TodayPoly- and perfluoroalkyl substances (PFAS) are rapidly emerging as some of the most important environmental contaminants to monitor around the world. Known as “forever che …

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15th Aug 2022

Which Type of Glass Vial is Best for your Application?

Glass autosampler vials are commonly used for inertness, but with the many varieties of glass options, how do you decide which type of glass vials are suitable for your application? In this blog, we’l …

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12th Jul 2022

How Does ICP-MS Work?

How Does an ICP-MS Work?Inductively coupled plasma mass spectrometry (ICP-MS) is a method used to determine which elements something is made of, as opposed to molecules and compounds that are measured …

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12th Jul 2022

Should You Use a Glass Pre-Filter Syringe Filter?

Should You be Using a Glass Pre-filter Syringe Filter?Proper sample filtration improves both the quality and consistency of analytical results, while also prolonging the lifetime of your instrument’s …

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2nd Jun 2022

Safety Waste Compliance in One Easy Step

Improper waste collection and handling of hazardous waste can put the people and property in a lab at risk. Long gone are the days of dumping HPLC solvent waste down the drain, but even experienced ch …

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2nd Jun 2022

Three Reasons You’ll Want to Switch to Agilent Capillary Tubing

Stainless steel tubing is the most popular material for HPLC and UHPLC due to it being compatible with many HPLC solvents, and able to withstand very high pressures. PEEK is the most common alternativ …

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12th May 2022

One Simple Step to Conserve Helium

As most people in the GC industry are well aware, the helium shortage is still in full swing. Just a few weeks back, we posted a blog about alternative carrier gases. But we know that not everyone can …

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12th May 2022

Revisiting ‘The Big Pain’

Throughout the pandemic, drug testing facilities were suddenly slammed with COVID-19 testing, meaning other testing (drug screening, etc.) was pushed to the wayside. This meant regular employee drug s …

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12th Apr 2022

What LC Column Should I Use for EtG and EtS Detection?

Ethyl glucuronide (EtG) and ethyl sulfate (EtS) biomarkers are formed by the liver after ethanol ingestion. They can be detected in urine approximately 2-80 hours after alcohol is consumed. Due to the …

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12th Apr 2022

Using 96 Well Plates In High Throughput Laboratories

Using 96 well plates instead of autosampler vials has significant advantages for high throughput labs, due to the ease of automation. Unfortunately, not all steps in the process can be fully automated …

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12th Apr 2022

Do You Really Need Helium as Your Carrier Gas?

Unpredictable helium supply can be problematic for chromatographers. With ongoing helium shortages, some labs are being rationed to 50% of their normal supply. Chrom Tech can help you find an alternat …

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10th Mar 2022

When to Use High Recovery Vials

There are two main reasons for labs to utilize high recovery vials. The first, and likely most obvious, is for limited-volume samples. In these cases, getting the last few microliters is crucial for s …

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6th Mar 2022

How Many Injections Can I Get on My HPLC Column?

It depends! Many factors affect how many injections you can get on your column, including: Injecting “dirty” samplesFollowing HPLC Column manufacturers recommended protocol/operating conditionsUsing …

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11th Feb 2022

Method Transfer between Poroshell and Zorbax HPLC Columns

One of the primary benefits to using superficially porous particle (SPP) HPLC columns, as opposed to totally porous particles is the results yield the efficiency of sub-2-µm, fully porous columns, wit …

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11th Feb 2022

How do Stainless-Steel Fittings Differ from Polymer-Based Fittings?

Both stainless-steel and polymer-based fittings are quite common in HPLC, and there are pros and cons for each. Deciding which to use for your lab depends on the application and requirements of your m …

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19th Jan 2022

True Crime and Chromatography

People have always been fascinated by mystery, and in recent years there has been a major rise in true crime series: podcasts, tv shows, and even books. The horrible, gruesome tragedies have become a …

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19th Jan 2022

Reduce Instrument Downtime with Sample Preparation

Reducing downtime in the laboratory saves time and money spent on getting the instrument back up and running. One easy way to reduce the downtime of your HPLC is to prolong the life of your HPLC colum …

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9th Dec 2021

Help! My Chromatography Vials are on Backorder!

As we all know, supply chain issues continue to cause issues in the lab. We’ve noticed an increase in people reaching out to see if we have certain vials in stock because they’re on backorder elsewher …

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9th Dec 2021

How to Connect a Syringe to HPLC Tubing

The need to connect a syringe to your LC pump to pull mobile phase through for priming (among other applications) is fairly common. We’ve seen some creative ways that people in the laboratory have con …

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15th Nov 2021

ASK BETHANY | Should I Use a Guard Column

HPLC ColumnsOne of the most important components in an LC system is the column, which is where analytes are separated out from a mixture for qualitative and quantitative analysis. HPLC columns need to …

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10th Nov 2021

What are the Benefits of Glass Vials in the Laboratory?

Chromatography vials are small glass or plastic vessels used in the lab to store and transport samples until they can be analyzed by HPLC or GC. Chances are, if you’re reading this, you probably do so …

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10th Nov 2021

An Essential Guide to HPLC Fittings for Your Laboratory

An Introduction to HPLC FittingsFittings are used any time tubing needs to be attached, adapted, or adjusted throughout the system, so creating good connections with fittings is extremely important fo …

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24th Oct 2021

Detecting Synthetic Opioids With an Agilent HPLC and Poroshell Column

Opioid abuse continues to pose a huge threat in the medical community. Unintentional overdose deaths involving opioid pain relievers have more than quadrupled since 1999 and have outnumbered those inv …

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20th Oct 2021

The 3 Most Common Applications for Headspace Vials

Gas chromatography is a technique widely used to separate volatile compounds in a mixture. This is done by using a flowing (inert, gaseous) mobile phase to carry the mixture through a solid stationary …

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11th Oct 2021

Getting to the Bottom of Vial Inserts

If you’ve got questions about chromatography vials, you’re in the right place. Vials are our specialty here at Chrom Tech, and we’ve got a number of blogs on the subject. For those of you who are tryi …

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29th Sep 2021

A Sneak Peak into the Largest HPLC Industry in the World - Biotechnology Applications

Biotechnology is a broad term, and as a whole is one of the largest industries in the world. The biotechnology field is responsible for the success of food and agriculture, medicine, genetic resear …

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29th Sep 2021

4 Questions You Should Ask Before Buying an HPLC Pump

Chrom Tech’s positive-displacement piston pumps are used for a wide variety of analytical, clinical, preparative, and fluid-metering applications. Many of our customers use our HPLC pumps for deliv …

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13th Sep 2021

ASK BEN | 4 Simple Steps to Find the Right GC Liner

We get a number of questions on GC liners, specifically what liner they should be using for their application. Choosing the appropriate GC inlet liner is crucial to ensure your sample can be proper …

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9th Sep 2021

Helpful Fluoropolymer Tubing Guide for Your HPLC Applications

What types of tubing are used in the laboratory? In liquid chromatography and other fluidic laboratory applications, you can choose from a wide variety of tubing materials, sizes, and other specific …

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7th Sep 2021

How to Properly Swage HPLC Fittings

The ability to change and properly connect fittings on an HPLC is an important skill to have if you’re a chromatographer. To do any HPLC work, you’ll inevitably need to change or tighten a connecti …

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29th Aug 2021

How Do You Dispose of HPLC Waste?

HPLC (High-Performance Liquid Chromatography) is used in many industries to separate complex mixtures of known or unknown analytes into individual components. With the use of internal or external stan …

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16th Aug 2021

How To Choose the Right Headspace Vial Cap

Why Choose Headspace GC?Headspace gas chromatography (GC) analysis is a method commonly used with volatile organic compounds, such as blood alcohol content, pharmaceuticals, and food flavoring analyse …

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