HPLC Columns
HPLC and UHPLC Columns
Chrom Tech is an authorized distributor and trusted supplier of HPLC and UHPLC columns from industry-leading manufacturers including Agilent Technologies, Concise Separations, Hamilton, Imtakt USA, ZirChrom, Regis, Restek, MilliporeSigma, Thermo Scientific, and HiChrom. Our portfolio delivers reliable performance and manufacturer-backed support for every separation challenge.
Achieve fast, consistent, and reproducible results for both small- and large-molecule workflows. Chrom Tech carries columns in a wide range of lengths, particle sizes, and stationary phases to match your analytical needs — from reversed phase and normal phase to chiral, HILIC, ion exchange, and SEC methods. Whether your work is in pharmaceutical, environmental, food, or research analysis, our technical team is ready to guide you toward the ideal column for your system.
For assistance or application guidance, contact Chrom Tech to connect with our chromatography specialists.
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HPLC Column Categories
Explore columns in a variety of lengths, particle sizes, and stationary phases to match your analytical needs
Shop Agilent HPLC Columns
Complete line of Agilent HPLC columns for small-molecule analysis. This broad portfolio supports nearly all separation modes and analytical applications.
Shop Agilent BioHPLC
Full range of columns for large-molecule and biopharmaceutical separations. Support protein, peptide, glycan, and antibody analysis with exceptional reproducibility and recovery.
Shop Agilent for GPC/SEC
Agilent GPC and SEC columns for accurate characterization of polymers, plastics, and macromolecules in aqueous, organic, and polar solvent systems. Reliable separation of molecular weights and distributions using both gel permeation chromatography (GPC) and size exclusion chromatography (SEC) techniques.
Shop Concise Separations
Specialized, application-focused chromatography solutions designed to optimize analyte separation and quantitation. Each Concise product line provides high selectivity and reproducibility for a targeted set of analytical workflows.
Shop Hamilton HPLC Columns/SEC
Leader in polymeric high-performance liquid chromatography (HPLC) columns for over 35 years, known for mechanical stability, long lifetime, and reliable performance under demanding pressure conditions.
Shop Imtakt Columns
Columns for superior separation efficiency and reproducibility, these HPLC columns feature proprietary silica and polymer chemistries that deliver excellent peak symmetry, high stability, and batch-to-batch consistency.
Shop Regis HPLC Columns
Advanced HPLC columns for both chiral and achiral separations, offering exceptional performance, reproducibility, and selectivity. Each column is engineered with proprietary stationary phases to meet the needs of pharmaceutical, environmental, and biochemical laboratories.
Shop Restek HPLC Columns
Trusted by analytical labs worldwide for their precision, speed, and reliability. Designed for both modern UHPLC and conventional HPLC systems.
Shop Thermo Fisher Scientific HPLC and UHPLC Columns
Comprehensive portfolios of HPLC and UHPLC columns available. Designed for analytical precision and operational stability, these columns deliver consistent results across a wide range of separation techniques and instrument platforms.
Shop ZirChrom HPLC Columns
Feature stationary phases engineered from porous zirconia particles that deliver exceptional chemical and thermal stability, outperforming traditional silica-based columns under extreme pH and temperature conditions.
Reversed-Phase Chromatography (RP)
The most widely used HPLC mode where the stationary phase is nonpolar (e.g., C18, C8) and the mobile phase is more polar. Ideal for small molecules, pharmaceuticals, and general analytical separations.
Normal-Phase Chromatography (NP)
A separation mode using a polar stationary phase (such as silica) and nonpolar mobile phases. Commonly applied to hydrophilic analytes and compounds that retain poorly by reversed phase.
HILIC (Hydrophilic Interaction Chromatography)
A hybrid mode using a polar stationary phase and high-organic mobile phase. Excellent for polar metabolites, sugars, nucleotides, and LC-MS applications requiring strong retention of hydrophilic compounds.
Chiral Chromatography
A separation technique using chiral stationary phases (CSPs) to resolve enantiomers. Essential for pharmaceutical and regulatory workflows where stereochemical purity matters.
Ion Exchange Chromatography (IEX)
A mode that separates charged analytes based on ionic interactions with cation or anion exchange stationary phases. Common for proteins, peptides, and biomolecules.
Size Exclusion Chromatography (SEC)
A mode that separates analytes based on molecular size using porous stationary phases. Widely used for polymer analysis, protein aggregation studies, and biotherapeutic characterization.
What types of HPLC columns does Chrom Tech offer?
Chrom Tech supplies a complete range of HPLC and UHPLC columns for reversed-phase, normal-phase, chiral, ion exchange, HILIC, and SEC separations — including popular series such as Agilent Poroshell, Restek Raptor, and Thermo Accucore.
Which brands of HPLC columns are available?
Chrom Tech is an authorized distributor for Agilent Technologies, Restek, Thermo Scientific, Hamilton, Concise Separations, Imtakt USA, and more — providing access to genuine, manufacturer-supported chromatography columns.
How do I select the right column for my method?
The right HPLC column depends on your sample chemistry and detection method. Chrom Tech specialists can recommend optimal stationary phases, pore sizes, and particle dimensions based on your analytes and instrument platform to achieve the best resolution and peak shape.
