HPLC Columns

HPLC and UHPLC Columns for Analytical Method Development and Routine Laboratory Workflows

Shop column options from Agilent, Thermo Scientific, Restek, Concise, Hamilton, Imtakt, Regis, and ZirChrom for reliable separations across a wide range of applications

HPLC Columns

Chrom Tech is an authorized distributor and trusted supplier of HPLC and UHPLC columns from industry-leading manufacturers including Agilent Technologies, Concise Separations, Hamilton, Imtakt USA, ZirChrom, Regis, Restek, MilliporeSigma, Thermo Scientific, and HiChrom. Our portfolio delivers reliable performance and manufacturer-backed support for every separation challenge.

Achieve fast, consistent, and reproducible results for both small- and large-molecule workflows. Chrom Tech carries columns in a wide range of lengths, particle sizes, and stationary phases to match your analytical needs — from reversed phase and normal phase to chiral, HILIC, ion exchange, and SEC methods. Whether your work is in pharmaceutical, environmental, food, or research analysis, our technical team is ready to guide you toward the ideal column for your system.

For assistance or application guidance, contact Chrom Tech to connect with our chromatography specialists.

LC Columns Resources

Explore practical guidance on HPLC and UHPLC column care, pressure control, particle and pore size selection, guard columns, and Poroshell method optimization.

HPLC Column Categories

Explore columns in a variety of lengths, particle sizes, and stationary phases to match your analytical needs

Products
Products
Products
Products
Products
Products
Products
Products
Products
Products
Key Definitions ▸
Reversed-Phase Chromatography (RP)
The most widely used HPLC mode where the stationary phase is nonpolar (e.g., C18, C8) and the mobile phase is more polar. Ideal for small molecules, pharmaceuticals, and general analytical separations.
Normal-Phase Chromatography (NP)
A separation mode using a polar stationary phase (such as silica) and nonpolar mobile phases. Commonly applied to hydrophilic analytes and compounds that retain poorly by reversed phase.
HILIC (Hydrophilic Interaction Chromatography)
A hybrid mode using a polar stationary phase and high-organic mobile phase. Excellent for polar metabolites, sugars, nucleotides, and LC-MS applications requiring strong retention of hydrophilic compounds.
Chiral Chromatography
A separation technique using chiral stationary phases (CSPs) to resolve enantiomers. Essential for pharmaceutical and regulatory workflows where stereochemical purity matters.
Ion Exchange Chromatography (IEX)
A mode that separates charged analytes based on ionic interactions with cation or anion exchange stationary phases. Common for proteins, peptides, and biomolecules.
Size Exclusion Chromatography (SEC)
A mode that separates analytes based on molecular size using porous stationary phases. Widely used for polymer analysis, protein aggregation studies, and biotherapeutic characterization.
Frequently Asked Questions ▸
What types of HPLC columns does Chrom Tech offer?
Chrom Tech supplies a complete range of HPLC and UHPLC columns for reversed-phase, normal-phase, chiral, ion exchange, HILIC, and SEC separations — including popular series such as Agilent Poroshell, Restek Raptor, and Thermo Accucore.
Which brands of HPLC columns are available?
Chrom Tech is an authorized distributor for Agilent Technologies, Restek, Thermo Scientific, Hamilton, Concise Separations, Imtakt USA, and more — providing access to genuine, manufacturer-supported chromatography columns.
How do I select the right column for my method?
The right HPLC column depends on your sample chemistry and detection method. Chrom Tech specialists can recommend optimal stationary phases, pore sizes, and particle dimensions based on your analytes and instrument platform to achieve the best resolution and peak shape.