Maximizing Throughput in an HPLC Laboratory

Increasing sample throughput is one of the most effective ways for laboratories to boost productivity and reduce operating costs. While optimizing HPLC analysis efficiency is crucial, cost savings can also be achieved by improving other aspects of the workflow—from sample preparation to consumable selection. Below, Chrom Tech outlines several practical strategies to help your lab maximize efficiency and make the most of every run.

Speed Up Sample Run Time

Reducing LC run times without sacrificing resolution is a key part of method optimization. Chromatographers can often achieve faster separations by making simple yet effective adjustments to their columns and method parameters. Here are a few proven techniques:

• Shorten the column length: Switching from a 100 mm to a 50 mm column can cut analysis time nearly in half.
• Use smaller particle sizes: Reducing particle size from 5 μm to 3 μm or even 1.8 μm can maintain resolution while increasing speed.
• Optimize flow rate and gradient: Carefully increasing the flow rate and adjusting gradient conditions can reduce analysis time, though users should monitor system backpressure.

For additional insight, review our guide to superficially porous particles, which explains how modern column technology can help maintain efficiency while managing higher backpressure.

Choose Fit-for-Purpose Sample Preparation Methods

Sample preparation can significantly impact both accuracy and total analysis time. The ideal method depends on sample complexity and desired precision. For clean samples, a simple “dilute-and-shoot” approach may suffice. However, as sample matrices become more complex, more robust preparation steps are needed.

One efficient option for improving sample quality is filtration. Using syringe filters or filter vials helps remove particulates, which protects the analytical column and reduces system downtime. In high-throughput settings, even incremental improvements in sample prep efficiency can yield substantial time and cost savings. Balancing preparation time against consumable costs ensures consistent, cost-effective results.

Save Money on High-Use Consumables

Consumables represent a significant portion of operational costs in analytical laboratories. Choosing cost-effective, high-quality products can have a lasting impact on your bottom line. Chrom Tech offers competitive pricing on frequently used items such as:

• Autosampler vials
• 96-well plates
• Filtration products

For laboratories processing hundreds or thousands of samples, well plates can be a game-changer. Using 96-well plates enables automation and reduces handling time, with a cost per sample that’s typically one-fifth that of traditional autosampler vials. Chrom Tech stocks a wide variety of plate configurations for different instruments and sample volumes—and when plates aren’t feasible, our extensive autosampler vial selection ensures a perfect fit for your workflow.

Maximize Your Spending Power in the Lab

Improving throughput doesn’t just save time—it directly enhances your lab’s profitability. From faster analyses to smarter consumable choices, each optimization step compounds over time. To explore tailored solutions for your laboratory’s needs, contact a Chrom Tech product specialist today. Our team of chromatography experts can help you find the right products and strategies to maximize both performance and cost efficiency.