Blog

20th Dec 2024

How to Connect a Syringe to HPLC Tubing

Connecting a syringe to your LC pump for tasks like priming the system (and other applications) is a common need. We've seen various inventive solutions in laboratories where people have connected the …

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20th Dec 2024

ASK BETHANY | Should I Use a Guard Column

HPLC ColumnsThe column is one of the most crucial components in an LC system, as it is where analytes are separated from a mixture for both qualitative and quantitative analysis. While HPLC colum …

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20th Dec 2024

An Essential Guide to HPLC Fittings for Your Laboratory

An Introduction to HPLC FittingsFittings are essential whenever tubing needs to be attached, adapted, or adjusted within the system, making secure connections crucial for successful chromatography. In …

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19th Dec 2024

A Sneak Peak into the Largest HPLC Industry in the World - Biotechnology Applications

Biotechnology is a vast field and one of the largest industries globally, playing a crucial role in food and agriculture, medicine, genetic research, and beyond. The ability to analyze compounds and d …

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19th Dec 2024

Helpful Fluoropolymer Tubing Guide for Your HPLC Applications

What types of tubing are used in the laboratory?In liquid chromatography and other fluidic laboratory applications, there is a broad range of tubing materials, sizes, and specifications to choose from …

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19th Dec 2024

How Do You Dispose of HPLC Waste?

High-Performance Liquid Chromatography (HPLC) is a widely used technique across various industries for separating complex mixtures of known or unknown analytes into individual components. By utilizing …

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16th Dec 2024

Inline Filters vs. Guard Columns for HPLC

Filters, such as inline filters, pre-column filters, and column guards, play a crucial role in protecting analytical columns from damage and premature failure in HPLC systems. Over time, chromatograph …

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16th Dec 2024

How To Make an HPLC Connection With PEEK Tubing

To make an HPLC connection with fingertight fittings, follow these steps: Slide the nut onto the tubing that will be inserted into the port. Place the ferrule onto the tubing just below the nut. For …

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4th Dec 2024

Offline vs. Online Degassing of HPLC Solvents

Gas bubbles and outgassing can significantly impact HPLC performance. Outgassing occurs when the amount of gas exceeds what the HPLC solvent can absorb. The solvent's ability to absorb gas depends on …

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4th Dec 2024

The Role of Chromatography in Cannabis Testing

Cannabis use in the U.S. is expanding rapidly as more states legalize its consumption. With this growth, mandatory testing has become required in many states to ensure consumer safety. The Farm Bill a …

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3rd Dec 2024

Reverse-Phase Liquid Chromatography vs. Hydrophilic Interaction Liquid Chromatography

Liquid chromatography can be performed using various techniques, including reverse-phase and hydrophilic interaction liquid chromatography (HILIC). This method of chromatography involves an HPLC pump …

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3rd Dec 2024

Chromatography Identifies Clinical Classifiers of COVID-19

The COVID-19 pandemic has caused unparalleled challenges worldwide due to the variability in symptoms and rapid transmission. As the virus continues to surge across the U.S., there is an increasing de …

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27th Nov 2024

Capillary Tubing Selection Guide for Agilent HPLC Systems

Chrom Tech is dedicated to simplifying the shopping experience for consumers. Replacing the capillary tubing connections in your HPLC system can seem daunting and complicated.To help, we've created a …

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26th Nov 2024

Reduce PFAS Background Interference and Contamination

According to the EPA, PFAS are long-lasting in both the environment and the human body, and exposure to these substances can result in negative health outcomes. Known as perfluorinated compounds …

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10th Jun 2024

Five Ways to Reduce Costs in Your Chromatography Workflow

How You Can Save Money in Your Chromatography Laboratory Chromatography is a powerful analytical technique, but costs can add up quickly. Lab managers are regularly challenged to keep costs to a min …

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16th May 2024

Pore Size vs. Particle Size in HPLC Columns

When selecting an  HPLC column, it is important to understand the different attributes and how they will affect your chromatography. Chemical properties, such as the type of surface of the s …

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27th Mar 2024

Are Your Autosampler Vials Compromising Your Results?

Exploring the Impact of Vial Quality on HPLC and GC Performance In the world of chromatography, choosing the appropriate instrumentation and accessories is essential for achieving accurate and rel …

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4th Jan 2024

Revolutionizing Your Chromatography | The Power of Poroshell and more

Are you frustrated with increasing backpressures from your current HPLC column? Do you wish you could achieve UHPLC results with HPLC instrumentation? Choosing the proper tools for success is importan …

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3rd Jan 2024

Unlocking the Benefits of Agilent Bond Elut HLB SPE

Discover how Agilent Bond Elut HLB SPE products can revolutionize your sample preparation process and provide optimal results.Understanding the Importance of Sample Preparation Sample prepar …

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7th Nov 2023

EHS Compliance: Safely Manage HPLC Solvent Waste

Many of the solvents, and even samples, used in high performance liquid chromatography (HPLC) are hazardous and/or flammable. Because of this, it’s important to mitigate health and safety risks t …

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15th Aug 2023

How to Save Money and Increase Accuracy on Sample Prep

Laboratories can instantly increase throughput and improve their sample preparation accuracy with automation, but generally automation is only common in high throughput laboratories. But, what if th …

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9th Mar 2023

Syringe Filters for HPLC Sample Preparation: A Guide to Choosing the Right Filter for Consistent and Accurate Results

Sample preparation is a crucial step in high-performance liquid chromatography (HPLC). While sample preparation can vary depending on the type of application and level of interference a sample contain …

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13th Feb 2023

How to Choose the Right Septa for Your Autosampler Vial Closure

When performing LC and GC analysis, you’ll need something to store and transport your samples prior to injection. Chromatography vials are the most common vessel, but come in a variety of shapes and s …

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18th Sep 2022

What is the Smallest Sample Volume You Can Use in 96 Well Plates?

For high throughput laboratories, 96-well plates offer a number of advantages over chromatography vials such as cost savings and easy automation capabilities. Different well plate types serve differen …

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15th Sep 2022

What Sample Prep is Best for Vitamin D Analysis?

Low level testing of endogenous vitamin and hormone levels in biological samples is the driving force behind the need for more sensitive and specific analytical methods. One example is vitamin D analy …

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12th Jul 2022

Should You Use a Glass Pre-Filter Syringe Filter?

Should You be Using a Glass Pre-filter Syringe Filter?Proper sample filtration improves both the quality and consistency of analytical results, while also prolonging the lifetime of your instrument’s …

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2nd Jun 2022

Safety Waste Compliance in One Easy Step

Improper waste collection and handling of hazardous waste can put the people and property in a lab at risk. Long gone are the days of dumping HPLC solvent waste down the drain, but even experienced ch …

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2nd Jun 2022

Three Reasons You’ll Want to Switch to Agilent Capillary Tubing

Stainless steel tubing is the most popular material for HPLC and UHPLC due to it being compatible with many HPLC solvents, and able to withstand very high pressures. PEEK is the most common alternativ …

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12th May 2022

Revisiting ‘The Big Pain’

Throughout the pandemic, drug testing facilities were suddenly slammed with COVID-19 testing, meaning other testing (drug screening, etc.) was pushed to the wayside. This meant regular employee drug s …

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6th Mar 2022

How Many Injections Can I Get on My HPLC Column?

It depends! Many factors affect how many injections you can get on your column, including: Injecting “dirty” samplesFollowing HPLC Column manufacturers recommended protocol/operating conditionsUsing …

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11th Feb 2022

How do Stainless-Steel Fittings Differ from Polymer-Based Fittings?

Both stainless-steel and polymer-based fittings are quite common in HPLC, and there are pros and cons for each. Deciding which to use for your lab depends on the application and requirements of your m …

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11th Feb 2022

Method Transfer between Poroshell and Zorbax HPLC Columns

One of the primary benefits to using superficially porous particle (SPP) HPLC columns, as opposed to totally porous particles is the results yield the efficiency of sub-2-µm, fully porous columns, wit …

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