How to Connect a Syringe to HPLC Tubing

In chromatography and LC applications, connecting a syringe to your pump for priming or maintenance is a common and essential task. Many labs have improvised solutions for this connection, but Chrom Tech provides a wide range of standard fittings and adapters specifically designed for secure, leak-free syringe connections. These fittings are available in durable materials such as stainless steel and high-performance polymers, eliminating the need for makeshift fixes and ensuring safe, repeatable performance.

"We have several standard products specifically designed to connect syringes to tubing—so you don’t need to spend time 'MacGyvering' your connection."

Connecting a Syringe to Your Pump

For most labs, a male luer syringe is readily available and frequently used for priming pumps. Chrom Tech offers both metal and polymer options for connecting these syringes to HPLC systems. The stainless-steel female luer adapter is designed to connect directly into a high-pressure 10-32 port, providing a reliable and durable interface between your syringe and LC system.

For those who prefer a lightweight, chemical-resistant polymer option, the polymeric female luer adapter offers the same functionality in an easy-to-handle design. It connects from a luer fitting to a 10-32 port and is ideal for applications requiring non-metallic materials.

Connecting a Syringe to Tubing

In some cases, rather than connecting directly into a high-pressure port, you may need to connect a syringe to flexible tubing. If you’re working with standard male luer syringes, here are two simple configurations:

• Option 1: Connect your syringe to 1/8” tubing using a female luer adapter with a ¼-28 end. The ¼-28 end connects to a union designed for 1/8” tubing. The second port of the union is used to attach your tubing—providing a secure, adaptable connection.

• Option 2: For 1/16” tubing, use a similar setup but with a union and fittings compatible with 1/16” tubing. This approach provides the same secure and functional connection while accommodating smaller tubing sizes.

Additional Luer Lock Connection Options

For applications requiring the connection of tubing to a female luer, Chrom Tech also offers a male luer to female ¼-28 adapter. This configuration allows seamless integration of tubing and fittings, giving you maximum flexibility for custom system setups.

If you’re uncertain about which adapter or configuration best fits your system, our Chrom Tech technical specialists can assist with product selection and compatibility. Contact our support team anytime for personalized assistance or recommendations.