Blog

19th Dec 2024

How To Choose the Right Headspace Vial Cap

Why Choose Headspace GC?Headspace gas chromatography (GC) is a widely used technique for analyzing volatile organic compounds, including blood alcohol content, pharmaceuticals, and food flavorings. In …

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16th Dec 2024

The Different Types of Well Plates: Polypropylene and 96 Well Plates With Glass Inserts

What are the 96 deep well plate dimensions?Chrom Tech 96 well microplates adhere to the ANSI/SLAS footprint standards, guaranteeing compatibility with all leading microplate readers and automation sys …

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16th Dec 2024

Inline Filters vs. Guard Columns for HPLC

Filters, such as inline filters, pre-column filters, and column guards, play a crucial role in protecting analytical columns from damage and premature failure in HPLC systems. Over time, chromatograph …

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16th Dec 2024

The Different 96 Well Plate Bottom Shapes

96-well plates are commonly used in high-throughput chromatography mass spectrometry labs, enabling automation from sample preparation to final analysis. Due to the wide range of applications and the …

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16th Dec 2024

The Advantages of Silane-Treated Vials

Silane-treated chromatography vials remove the active sites on the internal glass walls, reducing analyte adhesion to the vial. This is particularly important for quantitative analysis, especially at …

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16th Dec 2024

Fuel Ethanol Fermentation Analysis by HPLC

Fuel Ethanol Fermentation Analysis by HPLC Ethanol production has gained significant popularity globally as consumers seek alternative fuel options. Experts can produce ethanol by converting corn and …

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16th Dec 2024

What Column Should I Use for Carbohydrate Analysis?

Why Is Carbohydrate Analysis Important? The food science industry relies on HPLC analysis to ensure the quality, taste, and concentration of food products. Accurate carbohydrate analysis is essential, …

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16th Dec 2024

How To Make an HPLC Connection With PEEK Tubing

To make an HPLC connection with fingertight fittings, follow these steps: Slide the nut onto the tubing that will be inserted into the port. Place the ferrule onto the tubing just below the nut. For …

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16th Dec 2024

Vials vs. 96-Well Plates for Chromatography

Vials and 96-well plates are both crucial consumables in chromatography. While vials were once the industry standard, 96-well plates have gained popularity in recent years. Each offers disti …

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13th Dec 2024

Cost Savings on Autosampler Vials for Chromatography

As many research labs face substantial budget reductions, laboratory managers must make difficult decisions on how to cut costs without affecting throughput and result precision. Chrom Tech delivers t …

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13th Dec 2024

3 Ways to Reduce Long Lead Times

How many times have you encountered the phrase “supply chain issues” in the past year? Many labs have faced extended lead times on products, forcing managers to find creative solutions to …

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13th Dec 2024

Tips for How to Prime an HPLC Pump

Why Is It Important To Prime an HPLC Pump? Priming your HPLC pump is essential to ensure that only the desired solvent flows through the pump. This is particularly important when installing a new pump …

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13th Dec 2024

The Best Vials To Use for PFAS Testing

What Are PFAS? PFAS, or Per- and Polyfluoroalkyl Substances, are synthetic chemicals that have been extensively used across various industries, particularly in food packaging, outdoor gear, and firefi …

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13th Dec 2024

PEEK Tubing: What It Is and the Different Types

PEEK Tubing: What It Is and the Different Types Poly Ether Ether Ketone (PEEK) tubing is widely used in chromatography laboratories and is a popular alternative to stainless steel tubing. It …

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13th Dec 2024

What Causes Peak Tailing in HPLC

What Causes Peak Tailing in HPLC? To understand peak tailing, it’s important to first define a "good" peak shape. In chromatography, an ideal peak is symmetrical, often referred to as a Gaussian …

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13th Dec 2024

Understanding How mRNA Vaccines Are Produced

HPLC pumps used in the production of Lipid-based Nanoparticles (LNP) for drug delivery of mRNA-based vaccines  Although Chrom Tech is known for its chromatog …

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13th Dec 2024

What Is Endcapping in HPLC Columns

What Is Endcapping in HPLC Columns Endcapping the bonded phase of an HPLC column reduces silanol interactions and safeguards the silica support from dissolution. Various endcapping techniques are empl …

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13th Dec 2024

What Is the Difference Between UHPLC and HPLC?

The two main differences between UHPLC and HPLC are:        Maximum System Pressure Rating        Typical Particle Size Used in the HP …

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10th Dec 2024

How To Regenerate a C18 HPLC Column

Typical Regeneration Procedure for Reversed Phase Columns:Regularly cleaning your column can help extend its lifespan, reducing the need for frequent replacements of HPLC Columns.For columns that …

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10th Dec 2024

The Difference Between Superficially Porous and Fully Porous Particles

Why Use a Superficially Porous Particle for HPLC Columns?Superficially porous particles (SPPs) consist of a solid core surrounded by a thin, porous shell, unlike traditional fully porous particles, wh …

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10th Dec 2024

When to Change your HPLC Piston Seal

Example of polymer side vs. energizer side of seal. Note stainless steel energizer shown. Seal could have fluoropolymer O-ring energizer instead (black O-ring).It is advisable to replace the pist …

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10th Dec 2024

Using Hydrogen as a Carrier Gas for Gas Chromatography

Hydrogen as a Carrier Gas?Hydrogen is gaining popularity as a carrier gas in the GC field. In this blog, we will explore the reasons why an increasing number of laboratories are making the switch to h …

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10th Dec 2024

Which Pumps Are Used in HPLC?

Why High Pressure in HPLC? Over the past 20 years, there has been a shift towards using smaller particles in HPLC columns. When I first joined Chrom Tech in the 90s, most of the columns we sold had a …

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9th Dec 2024

What is ICP-MS Used For?

Inductively Coupled Plasma - Mass Spectrometer (ICP-MS) is a mouthful, but it's often referred to simply as ICP-MS. This advanced analytical instrument is designed to detect low and trace levels of me …

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9th Dec 2024

What Column Should I Use for Proteomics?

Proteins are fundamental to all living organisms, and as you can imagine, many industries, including pharmaceuticals, clinical research, and environmental sciences, rely on understanding them. One com …

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9th Dec 2024

How Does a Pulse Dampener Work in an HPLC Pump?

Chrom Tech provides a range of HPLC pumps, including positive-displacement piston pumps, which are suitable for HPLC, as well as analytical, clinical, preparative, and fluid-metering applications. The …

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9th Dec 2024

When To Use PEEK Tubing Over Stainless Steel Tubing in HPLC

PEEK tubing offers several advantages over stainless steel tubing in HPLC systems, but it also has some limitations. PEEK is a high-performance engineered polymer known for its mechanical an …

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9th Dec 2024

Is There a Universal Membrane for Syringe Filters?

Unfortunately, there is no single HPLC syringe filter membrane suitable for all applications, as different membranes possess distinct properties. The two key factors to consider are solvent compatibil …

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9th Dec 2024

What Types of Fittings Are Used in HPLC?

There are two main types of fittings used in HPLC systems. Low-pressure fittings are used to connect components up to the inlet of the HPLC pump, while high-pressure fittings are used from the pump ou …

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9th Dec 2024

What Are 96 Well Plates Used For?

96-well microplates (Deep Well Polypropylene Microplates) are widely used for sample storage in life science laboratories. They are particularly popular in high-throughput LCMS labs for sample prepara …

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9th Dec 2024

EPA Recommended HPLC Vial Wash Procedures

The EPA has established guidelines (section 4.1.4.1) for sample containers used in the analysis of semivolatile organic compounds (SVOCs). Containers for SVOC samples are typically made of g …

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9th Dec 2024

Are Hydrogen Generators Safe?

While hydrogen gas cylinders are commonly used in laboratories, they pose various safety risks and can lead to unexpected instrument downtimes due to inconsistent delivery schedules. These problems ca …

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9th Dec 2024

What Is the Difference Between a Union and an Adapter in HPLC?

Adapters are used to connect two different types of thread or port configurations, while unions feature the same thread or port configuration on both sides, making them generally more cost-effective. …

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9th Dec 2024

HPLC Column Care and Maintenance

Your analytical column is a consumable component of your chromatography system and will eventually need to be replaced. To extend the lifespan of your column, it is essential to properly care for and …

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9th Dec 2024

Why Use GC Derivatization Reagents

Gas Chromatography is employed to separate volatile organic compounds. Derivatizing reagents are utilized to modify the functional groups of analytes, facilitating their chromatographic separation and …

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4th Dec 2024

Acetonitrile vs. Methanol for Reverse Phase Chromatography

Acetonitrile (ACN) and methanol (MeOH) are the most widely used organic modifiers in reversed-phase chromatography. Each solvent has its own set of advantages and disadvantages. This guide provides a …

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4th Dec 2024

How to Streamline Your Sample Preparation Workflow

Sample preparation is essential for chromatography analysis. The goal is to ensure that the sample injected into the chromatography system is representative, reproducible, and homogeneous. In his 2013 …

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4th Dec 2024

How To Choose a Syringe Filter

Select Chrom Tech's affordable HPLC syringe filters to remove particulates from your liquid samples before injection into your HPLC system. Our syringe filters are cost-effective, single-use, and …

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4th Dec 2024

How to Choose a Pump for High-Pressure Solvent Applications

HPLC pumps, also known as solvent delivery pumps, are versatile tools used in various applications beyond chromatography. Chrom Tech’s high-pressure solvent delivery pumps provide consistent flow rate …

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4th Dec 2024

Why You Should Filter Mobile Phase HPLC

Mobile phase filtration is essential in HPLC. It not only extends the lifespan of your HPLC system but also helps ensure consistent, accurate, and repeatable results. Here are some of the key reasons …

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4th Dec 2024

Fundamentals of Sample Preparation for Chromatography

Sample preparation is a crucial step in chromatography analysis, involving the extraction of analytes of interest from the sample matrix. This process is essential for ensuring accurate analysis, mini …

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4th Dec 2024

Which 96-Well Plate Should I Use for LCMS?

Choosing the right 96-well plate depends on several factors, including whether it is rimmed or non-rimmed, its sample recovery capabilities, and the potential for sample binding.Rimmed vs. Non-Rimmed …

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4th Dec 2024

Essential List of Chromatography Supplies

Chromatography is a separation technique widely used by scientists to analyze complex mixtures, commonly applied in research and clinical laboratories. Popular methods include gas chromatography (GC) …

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4th Dec 2024

Offline vs. Online Degassing of HPLC Solvents

Gas bubbles and outgassing can significantly impact HPLC performance. Outgassing occurs when the amount of gas exceeds what the HPLC solvent can absorb. The solvent's ability to absorb gas depends on …

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4th Dec 2024

How To Maximize HPLC Column Lifetime

All chromatographic columns experience wear over time. Key factors that contribute to the degradation of HPLC columns include sample injections, as sample matrices may contain residual proteins, lipid …

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4th Dec 2024

The Role of Chromatography in Cannabis Testing

Cannabis use in the U.S. is expanding rapidly as more states legalize its consumption. With this growth, mandatory testing has become required in many states to ensure consumer safety. The Farm Bill a …

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4th Dec 2024

To Cut or Not-to-Cut

To Cut or Not to Cut: Is Paying Extra for Pre-Cut Stainless Steel Tubing Worth It?When setting up your HPLC system, it's crucial that the ends of your tubing are flat and free of burrs. This ensures t …

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4th Dec 2024

How To Reduce Column Pressure in HPLC

Pressure is a fundamental aspect of HPLC systems, as solvents are pumped through a packed bed of small particles. It's crucial for chromatographers to understand what constitutes "normal" pressure fo …

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