Blog

29th Sep 2021

4 Questions You Should Ask Before Buying an HPLC Pump

Chrom Tech’s positive-displacement piston pumps are used for a wide variety of analytical, clinical, preparative, and fluid-metering applications. Many of our customers use our HPLC pumps for deliv …

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29th Sep 2021

A Sneak Peak into the Largest HPLC Industry in the World - Biotechnology Applications

Biotechnology is a broad term, and as a whole is one of the largest industries in the world. The biotechnology field is responsible for the success of food and agriculture, medicine, genetic resear …

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13th Sep 2021

ASK BEN | 4 Simple Steps to Find the Right GC Liner

We get a number of questions on GC liners, specifically what liner they should be using for their application. Choosing the appropriate GC inlet liner is crucial to ensure your sample can be proper …

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9th Sep 2021

Helpful Fluoropolymer Tubing Guide for Your HPLC Applications

What types of tubing are used in the laboratory? In liquid chromatography and other fluidic laboratory applications, you can choose from a wide variety of tubing materials, sizes, and other specific …

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7th Sep 2021

How to Properly Swage HPLC Fittings

The ability to change and properly connect fittings on an HPLC is an important skill to have if you’re a chromatographer. To do any HPLC work, you’ll inevitably need to change or tighten a connecti …

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29th Aug 2021

How Do You Dispose of HPLC Waste?

HPLC (High-Performance Liquid Chromatography) is used in many industries to separate complex mixtures of known or unknown analytes into individual components. With the use of internal or external s …

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16th Aug 2021

How To Choose the Right Headspace Vial Cap

Why Choose Headspace GC?Headspace gas chromatography (GC) analysis is a method commonly used with volatile organic compounds, such as blood alcohol content, pharmaceuticals, and food flavoring analyse …

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16th Aug 2021

ASK BEN | How to Choose an HPLC or GC Syringe

Syringes are an essential part of HPLC and GC systems. In order to analyze a sample mixture on an LC column or GC column, the sample must be introduced into the flow path, and syringes make that po …

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11th Aug 2021

The Different 96 Well Plate Bottom Shapes

96 well plates are used in high-throughput chromatography mass spectrometry labs. 96 well plates allow for automation from sample preparation to final analysis. Because of the many applications …

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9th Aug 2021

The Advantages of Silane-Treated Vials

Silane-treated chromatography vials eliminate the active sites on the vial’s internal glass wall, which reduces adherence of analytes to the walls of the vial. When performing quantitative analysis, e …

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3rd Aug 2021

Fuel Ethanol Fermentation Analysis by HPLC

Fuel Ethanol Fermentation Analysis by HPLCEthanol production has become increasingly popular worldwide as consumers look for alternative fuel sources. Experts can convert corn and other renewable ener …

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14th Jul 2021

What Column Should I Use for Carbohydrate Analysis?

Why Is Carbohydrate Analysis Important?The food science industry relies heavily upon HPLC analysis for the quality, taste, and concentration of foods. In order to accurately analyze the carbohydrates …

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11th Jul 2021

How To Make an HPLC Connection With PEEK Tubing

For fingertight fittings, the followings steps should be taken to make an HPLC connection: Slip the nut over the tubing that will go into the port.Slide the ferrule over the tubing, just below the nu …

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27th Jun 2021

Extra HPLC Pump Parts You Need To Have on Hand

  HPLC pumps must be able to run trouble free 24/7/365. So, in order to keep your pump in “prime” condition, we recommend planning routine maintenance and keeping commo …

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20th Jun 2021

The Different Types of Well Plates: Polypropylene and 96 Well Plates With Glass Inserts

What are the 96 deep well plate dimensions?Chrom Tech 96 well microplates conform to the ANSI/SLAS footprint specifications, ensuring that they are compatible with all major microplate readers and aut …

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14th Jun 2021

3 Ways to Reduce Long Lead Times

How many times have you heard the words “supply chain issues” in the past year? We have heard of labs experiencing such long lead times on products that managers are forced to be very involved in f …

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13th Jun 2021

Tips for How to Prime an HPLC Pump

Why Is It Important To Prime an HPLC Pump?It is important to prime your HPLC pump to ensure that only the solvent you want flowing through the pump is present. Therefore, when installing a new pump, t …

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6th Jun 2021

The Best Vials To Use for PFAS Testing

What Are PFAS?PFAS, or Per- and Polyfluoroalkyl Substances, are man-made chemicals that have been widely used in many industries, but they are especially notable in food containers, outdoor gear, and …

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1st Jun 2021

What Causes Peak Tailing in HPLC

What Causes Peak Tailing in HPLCFirst, let’s explore what makes a “good” peak shape. In chromatography, a good peak shape is a symmetrical, or gaussian, peak. Good peaks with high efficiency and narro …

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25th May 2021

PEEK Tubing: What It Is and the Different Types

PEEK Tubing: What It Is and the Different TypesPoly Ether Ether Ketone (PEEK) tubing is common in chromatography laboratories and is a popular replacement for stainless steel tubing. PEEK tubing is bi …

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18th May 2021

What Is the Difference Between UHPLC and HPLC?

In short, there are two main differences between UHPLC and HPLC:        Maximum System Pressure Rating       Typical Particle Size Use …

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11th May 2021

Understanding How mRNA Vaccines Are Produced

HPLC pumps used in the production of Lipid-based Nanoparticles (LNP) for drug delivery of mRNA-based vaccines   Although Chrom Tech specializes in c …

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29th Apr 2021

What Is Endcapping in HPLC Columns

What Is Endcapping in HPLC ColumnsEndcapping of the HPLC Column bonded phase minimizes silanol interaction and protects the silica support from dissolution. Different endcapping procedures are utilize …

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15th Apr 2021

How To Regenerate a C18 HPLC Column

Typical Regeneration Procedure for Reversed Phase Columns:Cleaning your column periodically can extend its lifetime, which helps to reduce the frequency of purchasing new HPLC Columns.For columns that …

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11th Apr 2021

Three Ways Chrom Tech Celebrates Earth Day Every Day

On April 22nd, people across the globe will initiate sustainability efforts, connect with nature, and educate others on how we can reduce our carbon footprint in celebration of the 51st annual Earth D …

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28th Mar 2021

The Difference Between Superficially Porous and Fully Porous Particles

Why Use a Superficially Porous Particle for HPLC Columns?Superficially porous particles (SPPs) have a solid core surrounded by a thin, porous shell rather than porous throughout the entire particle, a …

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23rd Mar 2021

Using Hydrogen as a Carrier Gas for Gas Chromatography

Hydrogen as a Carrier Gas?Hydrogen is increasing in popularity in the GC world as a carrier gas. In this blog, will explore why more and more laboratories are turning to hydrogen.Renewable resourceOft …

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21st Mar 2021

Which Pumps Are Used in HPLC?

In order to understand which pumps are used in HPLC, we will first explore why high pressure is needed in HPLC.Why High Pressure in HPLC?Over the past 20 years, the trend has been to pack HPLC columns …

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14th Mar 2021

When to Change your HPLC Piston Seal

Example of polymer side vs. energizer side of seal. Note stainless steel energizer shown. Seal could have fluoropolymer O-ring energizer instead (black O-ring). It is recommended to change …

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7th Mar 2021

The Chrom Tech Way: A message from the President

Experience MattersChrom Tech hires chromatographers to sell chromatography consumables. 100% of our salesforce has work experience in a chromatography lab. We speak your language and understand the ch …

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2nd Mar 2021

What is ICP-MS Used For?

Inductively Coupled Plasma - Mass Spectrometer; that is a mouthful. ICP-MS for short is a more recent advancement in analytical instrumentation designed to detect low and trace levels of metals in var …

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13th Feb 2021

How Does a Pulse Dampener Work in an HPLC Pump?

Chrom Tech offers various HPLC pumps; these positive-displacement piston pumps are used for HPLC applications as well as analytical, clinical, preparative, and fluid-metering applications. The pistons …

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11th Feb 2021

What Column Should I Use for Proteomics?

Proteins make up every living organism and as you can imagine, many industries (pharmaceutical, clinical, environmental, etc.) rely on understanding them. One means of analyzing a protein is through L …

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6th Feb 2021

When To Use PEEK Tubing Over Stainless Steel Tubing in HPLC

PEEK tubing has advantages over stainless steel tubing when plumbing your HPLC system, but it also has its limitations. PEEK is a high-performance engineered polymer with mechanical- and chemical …

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30th Jan 2021

Is There a Universal Membrane for Syringe Filters?

Unfortunately, there isn’t a universal HPLC syringe filter membrane that will work for all applications, as different membranes have different properties. The two most important characteristics to und …

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23rd Jan 2021

What Types of Fittings Are Used in HPLC?

There are two types of fittings commonly found in all HPLC systems. Low-pressure fittings are used to make connections up to the inlet of the HPLC pump, and high-pressure fittings are utilized to make …

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16th Jan 2021

EPA Recommended HPLC Vial Wash Procedures

The EPA has set guidelines (section 4.1.4.1) for sample containers for Analysis of Semivolatile Organics: The containers specified for samples intended for analysis of SVOCs are typically constructed …

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16th Jan 2021

Pore Size vs. Particle Size in HPLC Columns

When selecting an HPLC column, it is important to understand the different attributes and how they will affect your chromatography. Chemical properties, such as the type of surface of the stationary p …

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16th Jan 2021

What Are 96 Well Plates Used For?

96 well microplates (Deep Well Polypropylene Microplates) are commonly used for sample storage in life science laboratories. They are commonly used in high-throughput LCMS laboratories for sample prep …

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11th Jan 2021

Are Hydrogen Generators Safe?

Although hydrogen gas cylinders are commonly found in the laboratory, they present a variety of safety risks and unexpected instrument downtimes due to inconsistent delivery schedules. These issues ca …

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30th Dec 2020

What Is the Difference Between a Union and an Adapter in HPLC?

Basically, adapters “adapt” between two different types of thread/port configurations, while unions have the same thread/port configuration on both sides (which makes unions typically more economical) …

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17th Dec 2020

HPLC Column Care and Maintenance

Your analytical column is a consumable in your chromatography system, and it must ultimately be replaced. To maximize your column’s lifetime, it is important to take care of and maintain your column. …

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16th Dec 2020

Why Use GC Derivatization Reagents

Gas Chromatography is used to separate volatile organic compounds. Derivatizing reagents are used to modify an analyte’s functional groups to enable chromatographic separation and/or detection. Benefi …

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9th Dec 2020

How to Streamline Your Sample Preparation Workflow

Sample preparation is critical to chromatography analysis; the objective is to have a sample injected on to the chromatography system that is representative, reproducible, and homogenous. In Ron Major …

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7th Dec 2020

How To Choose a Syringe Filter

Choose Chrom Tech economical HPLC syringe filters to remove particulates from your liquid samples prior to injection on your HPLC. Our syringe filters are economical and designed to be single-use …

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2nd Dec 2020

How to Choose a Pump for High-Pressure Solvent Applications

HPLC pumps or solvent delivery pumps can be used for many applications, not just specific to chromatography applications. Chrom Tech’s high-pressure solvent delivery pumps deliver constant flow rates …

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29th Nov 2020

What Is the Difference Between Liquid Liquid Extraction and Supported Liquid Extraction?

What Is Liquid Liquid Extraction?Liquid liquid extraction (LLE) is a labor-intensive method in which you are separating compounds into two immiscible liquids: the aqueous layer and an organic solvent …

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24th Nov 2020

How To Reduce Column Pressure in HPLC

By definition, pressure is inherent in HPLC systems because solvent is being pumped through a packed bed of small particles. It is important for chromatographers to understand what “normal” pressure i …

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