Poroshell StableBond 300 C3, 1.0 x 75 mm, 5 µm
Agilent Poroshell columns are ideal for fast separations of proteins and peptides because the unique particle allows for fast flow rates to be used while maintaining sharp, efficient peaks. Peptides and proteins are typically separated slowly to reduce the potential peak broadening of these slow diffusing analytes. But Poroshell columns use a unique particle made with a layer of porous silica on a solid core of silica. This reduces the diffusion distance for proteins making practical rapid HPLC separations of peptides and proteins up to 500–1,000 kDA. Poroshell 300SB C18, C8, C3, and 300Extend columns provide excellent stability and selectivity choices with TFA and formic acid mobile faces. Poroshell 300Extend C18 columns can be used at varying pH levels for unique separations and additional selectivity choices.
- UHPLC separations of biomolecules with superficially porous particles
- 300Å pore provide high efficiency and recovery with proteins (up to 1,000 kDa) and monoclonal antibodies
- Achieve long lifetime at low pH with Poroshell 300SB; at high pH with 300Extend-C18
- Optimize recovery and selectivity with four different bonded phases – 300SB-C18, 300SB-C8, 300SB-C3, and 300Extend-C18
| Brochures | |
|---|---|
Agilent HPLC ColumnsColumns for HPLC applications | Download |
InfinityLab Poroshell 134Column Selector Poster | Download |
| Blogs | |
How to Maximize HPLC Column LifetimeMaximize HPLC column lifetime to proactively avoid premature degredation. | |
Pore Size vs. Particle Size in HPLC ColumnsUnderstand the differences of HPLC column specifications | |
Optimizing HPLC Methods with Agilent's InfinityLab Poroshell 120 ColumnsMore chemistries, more separation, more consistency | |
- Column Family:
- Agilent Poroshell 300
- Phase:
- 300SB-C3
- ID:
- 1.0 mm
- Length:
- 75 mm
- Unit of Measure:
- EA
- Catalog Page Number:
- 78
- Type:
- HPLC Column
- Guard Column:
- No
- Hardware:
- SS
- Inner Diameter (ID):
- 1 mm
- LC Platform:
- Analytical - Stainless Steel, HPLC ( 600 bar)
- Pressure Rating:
- 400 bar
- LC Column Brand:
- Poroshell 300
- Particle Size:
- 5 um
- LC Column Maximum Temperature:
- 90 C
- LC Column Phase:
- 300SB-C3
- Particle Type:
- Superficially Porous
- pH Range:
- 1-8
- Pore Size:
- 300 A
- Separation Mode:
- Reversed Phase
- Shipping Solvent:
- Methanol/Water
- Part Number Application:
- Intact & Subunit Analysis;Monoclonal Antibodies (mABs);Proteins
- Part Number Application Domain:
- Biopharma
- Technique:
- LC
