ZORBAX Bonus-RP Columns
Agilent ZORBAX Bonus-RP Columns
Agilent ZORBAX Bonus-RP columns deliver exceptional reversed-phase performance for challenging basic compounds, offering a unique selectivity profile that extends well beyond traditional C18 and C8 phases. Engineered with an embedded polar amide group and triple endcapping, these columns minimize unwanted secondary interactions with silica, ensuring cleaner peak shapes, improved resolution, and superior reproducibility at low and mid-pH conditions.
- Excellent peak shape for difficult basic analytes—even under acidic and mid-pH conditions.
- Unique reversed-phase selectivity that complements and extends beyond conventional C18 and C8 columns.
- Embedded polar amide group + steric protection for reduced silanol interactions and improved stability.
- Fully compatible with 100% aqueous mobile phases for robust retention of polar analytes.
The Bonus-RP bonding chemistry combines a long alkyl chain with an internal amide group, dramatically reducing interaction between basic compounds and exposed silica sites. This design enhances peak symmetry for amines, heterocycles, and other problematic analytes. Diisopropyl side chains provide steric shielding against acid hydrolysis, improving durability under low-pH conditions where conventional columns rapidly degrade.
Whether you're developing new methods or optimizing difficult separations, ZORBAX Bonus-RP provides a powerful alternative selectivity option with excellent lifetime, stability, and consistency across complex sample types.
For assistance selecting the right ZORBAX column for your workflow, contact Chrom Tech and our technical specialists will help you find the best fit for your application.
Embedded Polar Group (EPG)
A polar functional group inserted within the alkyl chain of a reversed-phase ligand. EPG bonding reduces unwanted interactions with silanols, improves peak shape for basic compounds, and allows stable operation in highly aqueous mobile phases.
Steric Protection
The use of bulky side groups—such as diisopropyl moieties—to shield the silica–ligand bond from acid hydrolysis. This increases column lifetime under low pH conditions and enhances long-term stability.
Triple Endcapping
A surface treatment process that neutralizes residual silanol groups, resulting in superior peak shape—particularly for basic analytes—and increased reproducibility across mobile phase conditions.
100% Aqueous Compatibility
The ability of a stationary phase to maintain retention and avoid collapse in pure water mobile phases, enabling reliable analysis of highly polar analytes without method instability.
Alternate Selectivity
A separation profile distinct from traditional C18 or C8 phases. Bonus-RP provides unique retention characteristics for aromatic, basic, and polar compounds, enabling better resolution when conventional phases fall short.
What makes Agilent ZORBAX Bonus-RP columns different from traditional C18 phases?
Bonus-RP columns feature an embedded polar amide group and sterically protective side chains that minimize silica interactions, dramatically improving peak shape for difficult basic compounds and delivering alternate reversed-phase selectivity not achievable with conventional C18 or C8 phases.
Can Bonus-RP columns operate in 100% aqueous mobile phases?
Yes. The embedded polar group stabilizes retention in fully aqueous conditions, preventing phase collapse and enabling reliable separations for very polar and early-eluting analytes.
Are Bonus-RP columns suitable for low-pH separations?
Yes. Diisopropyl side groups protect the bonded phase from acid hydrolysis, offering strong durability and extended column lifetime under low-pH conditions where many silica phases degrade quickly.
When should I choose Bonus-RP over SB-C18 or Eclipse Plus?
Choose Bonus-RP when you need alternate RP selectivity, better retention of polar analytes, or significantly improved peak shape for strongly basic compounds—especially in low-to-mid pH mobile phases where silica interactions are most problematic.
Product List
| SKU | Name | LC Column Phase | Particle Size | Inner Diameter (ID) | Length |
|---|---|---|---|---|---|
SKU
883725-901
|
Name
883725-901 - Bonus-RP, 5u, 2.1x150mm, HPLC Column
|
LC Column Phase
Bonus-RP
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
150 mm
|
SKU
884950-577
|
Name
884950-577 - Bonus-RP,4.6x250mm,3.5um
|
LC Column Phase
Bonus-RP
|
Particle Size
3.5 um
|
Inner Diameter (ID)
4.6 mm
|
Length
250 mm
|
SKU
883668-901
|
Name
883668-901 - Bonus-RP, 5u, 4.6x150mm, HPLC column
|
LC Column Phase
Bonus-RP
|
Particle Size
5 um
|
Inner Diameter (ID)
4.6 mm
|
Length
150 mm
|
SKU
883668-301
|
Name
883668-301 - Bonus-RP 3.0x150mm 5u
|
LC Column Phase
Bonus-RP
|
Particle Size
5 um
|
Inner Diameter (ID)
3 mm
|
Length
150 mm
|
SKU
880668-901
|
Name
880668-901 - Bonus-RP, 5u,4.6x250mm, HPLC Column
|
LC Column Phase
Bonus-RP
|
Particle Size
5 um
|
Inner Diameter (ID)
4.6 mm
|
Length
250 mm
|
SKU
880668-301
|
Name
880668-301 - Bonus-RP 3.0x250mm 5u
|
LC Column Phase
Bonus-RP
|
Particle Size
5 um
|
Inner Diameter (ID)
3 mm
|
Length
250 mm
|
SKU
878250-101
|
Name
878250-101 - PrepHT,Zorbax,BonusRP,21.2x250mm,7uCrt
|
LC Column Phase
Bonus-RP
|
Particle Size
7 um
|
Inner Diameter (ID)
21.2 mm
|
Length
250 mm
|
SKU
878150-101
|
Name
878150-101 - PrepHT,Zorbax,BonusRP,21.2x150mm,7uCrt
|
LC Column Phase
Bonus-RP
|
Particle Size
7 um
|
Inner Diameter (ID)
21.2 mm
|
Length
150 mm
|
SKU
868150-901
|
Name
868150-901 - PrepHT,Zorbax,BonusRP,21.2x150mm,5uCrt
|
LC Column Phase
Bonus-RP
|
Particle Size
5 um
|
Inner Diameter (ID)
21.2 mm
|
Length
150 mm
|
SKU
868100-901
|
Name
868100-901 - PrepHT,Zorbax,BonusRP,21.2x100mm,5uCrt
|
LC Column Phase
Bonus-RP
|
Particle Size
5 um
|
Inner Diameter (ID)
21.2 mm
|
Length
100 mm
|
SKU
868050-901
|
Name
868050-901 - PrepHT,Zorbax,BonusRP,21.2x50mm,5uCrt
|
LC Column Phase
Bonus-RP
|
Particle Size
5 um
|
Inner Diameter (ID)
21.2 mm
|
Length
50 mm
|
SKU
864668-901
|
Name
864668-901 - Zorbax Bonus-RP, 3.5um, 4.6 x 100mm
|
LC Column Phase
Bonus-RP
|
Particle Size
3.5 um
|
Inner Diameter (ID)
4.6 mm
|
Length
100 mm
|