ZORBAX 80Å StableBond

Agilent ZORBAX 80Å StableBond HPLC Columns

  • Industry-leading stability at low pH — engineered for long, reliable lifetimes in acidic mobile phases down to pH 1.
  • Patented sterically protected siloxane bond delivers rugged performance under high temperature and harsh acidic conditions.
  • Six bonded phases (SB-C18, SB-C8, SB-CN, SB-Phenyl, SB-C3, SB-Aq) provide broad selectivity for method development.
  • High-purity Type B silica ensures sharp peaks, low bleed, and excellent reproducibility.

Agilent ZORBAX 80Å StableBond (SB) columns are the gold standard for low-pH reversed-phase separations. Built with patented nonfunctional silanes featuring bulky di-isobutyl or di-isopropyl side groups, the SB family sterically protects the critical siloxane bond from hydrolysis—ensuring unmatched stability where conventional silica columns rapidly degrade.

Because StableBond phases are not endcapped, they maintain maximum low-pH robustness and deliver highly reproducible retention for acids, bases, and neutrals. This makes the SB series ideal for pharmaceutical assays, stability-indicating methods, impurity profiling, and any workflow requiring extended operation at pH 1–2.

With six selectivity options and reliable performance across a wide range of temperatures and mobile phases, ZORBAX StableBond columns provide a dependable platform for accelerated method development and long-term routine analyses.

For guidance on choosing the best StableBond phase or exploring Chrom Tech’s full LC column portfolio, contact our technical specialists.

Key Definitions

Low-pH Stability

The ability of a column to operate in strongly acidic mobile phases (down to pH 1) without phase loss or silica hydrolysis. StableBond columns are engineered specifically for long lifetime under these harsh conditions.

StableBond (SB) Chemistry

A patented Agilent bonding technology using nonfunctional silanes with bulky side groups that sterically shield the siloxane bond from hydrolytic attack, providing superior durability at low pH and elevated temperatures.

Bonded Phase Selectivity

The chromatographic selectivity created by surface chemistry (C18, C8, CN, Phenyl, C3, Aq). StableBond offers six distinct phases to tune interactions and optimize separation of acids, bases, neutrals, and polar analytes.

Type B Silica

High-purity silica with low metal content, resulting in improved peak shape for basic analytes and reduced secondary interactions—critical for reproducible, high-efficiency separations.

Non-Endcapped Phase

StableBond columns intentionally skip endcapping to maximize low-pH durability and stability, creating stronger bonding and reproducibility under acidic mobile phase conditions.

Frequently Asked Questions

What makes ZORBAX StableBond columns ideal for low-pH separations?

StableBond columns use patented sterically protected alkyl silanes that shield the silica surface from hydrolytic attack, enabling long lifetimes and exceptional reproducibility even at pH 1 and elevated temperatures.

Are StableBond columns endcapped?

No. StableBond phases are intentionally non-endcapped to maximize their resistance to harsh acidic conditions and provide reproducible retention under aggressive mobile-phase environments.

Which bonded phases are available in the ZORBAX 80 Å StableBond family?

The StableBond family includes SB-C18, SB-C8, SB-C3, SB-Phenyl, SB-CN, and SB-Aq—offering broad selectivity and method-development coverage for acids, bases, neutrals, and high-aqueous workflows.

Can StableBond columns be used at high temperatures?

Yes. The patented bonding chemistry provides excellent thermal stability, making StableBond phases well-suited for fast, high-temperature LC methods without loss of performance.

Product List

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SKU Name LC Column Phase Particle Size Inner Diameter (ID) Length
SKU
877250-102
Name
877250-102 - PrepHT,Zorbax,SB-C18,21.2x250mm,7uCrt
LC Column Phase
SB-C18
Particle Size
7 um
Inner Diameter (ID)
21.2 mm
Length
250 mm
SKU
871700-914
Name
871700-914 - SB-Aq Rapid Res Col 2.1 x 50mm, 3.5um
LC Column Phase
SB-Aq
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
877150-114
Name
877150-114 - PrepHT,Zorbax,SB-Aq,21.2x150mm,7uCrt
LC Column Phase
SB-Aq
Particle Size
7 um
Inner Diameter (ID)
21.2 mm
Length
150 mm
SKU
871700-906
Name
871700-906 - ZORBAX SB-C8,2.1x50mm,3.5um
LC Column Phase
SB-C8
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
877150-106
Name
877150-106 - PrepHT,Zorbax,SB-C8,21.2x150mm,7uCrt
LC Column Phase
Rx-C8
Particle Size
7 um
Inner Diameter (ID)
21.2 mm
Length
150 mm
SKU
871700-902
Name
871700-902 - ZORBAX SB-C18,2.1x50mm,3.5um
LC Column Phase
SB-C18
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
877150-102
Name
877150-102 - PrepHT,Zorbax,SB-C18,21.2x150mm,7uCrt
LC Column Phase
SB-C18
Particle Size
7 um
Inner Diameter (ID)
21.2 mm
Length
150 mm
SKU
874700-906
Name
874700-906 - SB-C8,2.1x30mm,1.8um,600bar
LC Column Phase
SB-C8
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
30 mm
SKU
861954-314
Name
861954-314 - SB-Aq 3.0 x 100mm 3.5u
LC Column Phase
SB-Aq
Particle Size
3.5 um
Inner Diameter (ID)
3 mm
Length
100 mm
SKU
859700-902
Name
859700-902 - RRHD SB-C-18, 2.1x150mm, 1.8um, 1200 bar
LC Column Phase
SB-C18
Particle Size
1.8 um
Inner Diameter (ID)
2.1 mm
Length
150 mm
SKU
861954-312
Name
861954-312 - SB-Phenyl 3.0 x 100mm 3.5u
LC Column Phase
SB-Phenyl
Particle Size
3.5 um
Inner Diameter (ID)
3 mm
Length
100 mm
SKU
861954-309
Name
861954-309 - SB-C3 3.0 x 100mm 3.5u
LC Column Phase
SB-C3
Particle Size
3.5 um
Inner Diameter (ID)
3 mm
Length
100 mm