Zorbax 300A Stablebond

Agilent ZORBAX 300Ã… StableBond HPLC Columns

Agilent ZORBAX 300Ã… StableBond columns are engineered for high-performance separations of proteins, peptides, and other large biomolecules. Their wide-pore (300Ã…) design ensures that larger analytes fully access the bonded phase, enabling sharper peaks, improved recovery, and high-efficiency reversed-phase separations across complex biological samples.

Built on Agilent’s patented StableBond low-pH-resistant chemistry, these columns deliver exceptional stability and reproducibility when used with acidic mobile phases—particularly those containing TFA, formic acid, or acetic acid. This robustness makes them the preferred option for LC/MS workflows where low pH is required for sensitivity, ionization performance, and chromatographic resolution.

  • Optimized bonded phases for peptide and protein selectivity (SB-C18, SB-C8, SB-Phenyl, SB-CN)
  • Unmatched low-pH durability with TFA-rich mobile phases—ideal for peptide mapping and protein profiling
  • Excellent reproducibility for regulated and high-precision LC/MS workflows
  • Available in analytical, capillary, and nano formats for proteomics, bio-pharma, and LC/MS applications

Whether you are analyzing intact proteins, peptide digests, biotherapeutics, or high-molecular-weight biomolecules, ZORBAX 300Ã… StableBond columns provide the stability, selectivity, and reliability essential for modern bio-separations.

For assistance selecting the right phase or geometry for your workflow, contact Chrom Tech.

Agilent ZORBAX 300Å StableBond — Phase Descriptions ▸
ZORBAX 300SB-C18
300 Å • StableBond • 90 °C • pH 1–8 • USP L1 • Protein optimized
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300SB-C18 is the primary reversed-phase choice for intact proteins and large peptides. Its 300 Ã… pore size enables efficient mass transfer of high-molecular-weight biomolecules while StableBond chemistry ensures durability under acidic, high-temperature conditions.

Separation ModeReversed Phase (C18)
Pore Size300 Ã…
Temperature Limit90 °C
pH Range1 – 8
USP DesignationL1
ZORBAX 300SB-C8
300 Å • StableBond • 90 °C • pH 1–8 • USP L7
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300SB-C8 provides reduced hydrophobic retention compared to C18, offering faster elution of large proteins while maintaining resolution. Ideal when C18 retains too strongly.

ZORBAX 300SB-C3
300 Å • StableBond • Low hydrophobicity
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300SB-C3 minimizes hydrophobic interaction, improving recovery of very large or highly hydrophobic proteins. Often selected for intact mAbs or fragile biomolecules.

ZORBAX 300SB-CN
300 Å • Cyanopropyl • Alternative selectivity • USP L10
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300SB-CN provides different selectivity for proteins and peptides when alkyl phases do not resolve critical pairs. Useful for altering retention order without changing separation mode.

ZORBAX 300SB-Diphenyl
300 Å • Aromatic selectivity • USP L11
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300SB-Diphenyl introduces π–π interactions in addition to hydrophobic retention, improving resolution of aromatic proteins or closely related structural variants.

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SKU Name LC Column Phase Particle Size Inner Diameter (ID) Length
SKU
857750-944
Name
857750-944 - ZORBAX RRHD 300-Diphenyl 2.1 x50 mm, 1.8 um
LC Column Phase
300SB-Diphenyl
Particle Size
1.8 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
865973-909
Name
865973-909 - 300SB-C3 Rapid Res HPLC Col 4.6 x 50
LC Column Phase
300SB-C3
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
50 mm
SKU
863630-906
Name
863630-906 - 300SB-C8, 1 x 150 mm, 3.5 micron
LC Column Phase
300SB-C8
Particle Size
3.5 um
Inner Diameter (ID)
1 mm
Length
150 mm
SKU
857750-909
Name
857750-909 - ZORBAX RRHD300SB-C3 2.1 x50 mm, 1.8 um
LC Column Phase
300SB-C3
Particle Size
1.8 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
865973-906
Name
865973-906 - 300SB-C8 Rapid Res HPLC Col 4.6 x 50
LC Column Phase
300SB-C8
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
50 mm
SKU
863630-902
Name
863630-902 - 300SB-C18, 1 x 150 mm, 3.5 micron
LC Column Phase
300SB-C18
Particle Size
3.5 um
Inner Diameter (ID)
1 mm
Length
150 mm
SKU
857750-906
Name
857750-906 - ZORBAX RRHD 300SB-C8 2.1X50mm, 1.8um
LC Column Phase
300SB-C8
Particle Size
1.8 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
865973-905
Name
865973-905 - 300SB-CN Rapid Res HPLC Col 4.6 x 50
LC Column Phase
300SB-CN
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
50 mm
SKU
857750-902
Name
857750-902 - ZORBAX 300SB-C18 2.1x50mm 1.8um
LC Column Phase
300SB-C18
Particle Size
1.8 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
865973-902
Name
865973-902 - 300SB-C18 Rapid Res HPLC Col 4.6 x 50
LC Column Phase
300SB-C18
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
50 mm
SKU
865750-906
Name
865750-906 - 300SB-C8, 2.1 x 50 mm, 3.5micron
LC Column Phase
300SB-C8
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
860950-909
Name
860950-909 - 300SB-C3 Analytical Column 4.6 x 50
LC Column Phase
300SB-C3
Particle Size
5 um
Inner Diameter (ID)
4.6 mm
Length
50 mm
Key Definitions â–¸
300 Ã… Pore Size
A large pore diameter optimized for intact proteins, monoclonal antibodies (mAbs), and large peptide fragments. Wider pores reduce restricted diffusion and improve mass transfer for high-molecular-weight biomolecules.
StableBond (SB) Technology
Agilent’s low-pH–resistant bonding chemistry that enhances durability under acidic mobile phases and elevated temperatures. StableBond phases resist hydrolysis and provide long-term retention stability for protein separations.
C18 / C8 / C3 Selectivity
Alkyl-bonded stationary phases providing varying hydrophobic interaction strength. C18 offers strongest retention, C8 provides moderate retention, and C3 minimizes hydrophobic interaction for very large or fragile proteins.
Cyanopropyl (CN) Phase
A polar-embedded stationary phase offering alternative selectivity compared to alkyl phases. CN can change retention order and improve resolution of closely related proteins or peptides.
Diphenyl Phase
A stationary phase that introduces π–π interactions in addition to hydrophobic retention. Useful for aromatic peptides, proteins with aromatic residues, and resolving structural variants.
High-Temperature Operation (Up to 90 °C)
ZORBAX 300Ã… StableBond columns tolerate elevated temperatures, improving mass transfer, reducing solvent viscosity, and enhancing resolution of large biomolecules.
Frequently Asked Questions â–¸
What are ZORBAX 300Ã… StableBond columns designed for?
ZORBAX 300Ã… StableBond columns are engineered for intact proteins, monoclonal antibodies, and large peptides. The 300 Ã… pore size supports efficient mass transfer of high-molecular-weight biomolecules while maintaining strong reversed-phase retention.
What makes StableBond technology different?
StableBond (SB) chemistry is optimized for low-pH mobile phases and high-temperature operation. It resists bonded-phase hydrolysis and provides long-term retention stability under acidic gradient conditions commonly used in protein analysis.
How do I choose between C18, C8, and C3?
Choose C18 for strongest hydrophobic retention and general protein separations. Select C8 when C18 retains too strongly and faster elution is needed. Use C3 for very large or hydrophobic proteins where minimal interaction improves recovery.
When should I use CN or Diphenyl instead of alkyl phases?
CN and Diphenyl phases provide alternative selectivity. CN can alter retention order for closely related species, while Diphenyl introduces π–π interactions useful for aromatic peptides and structural variant resolution.
Are ZORBAX 300Ã… StableBond columns compatible with LC/MS?
Yes. These columns are widely used in LC/MS workflows for protein and peptide characterization, including intact mass analysis and peptide mapping under acidic conditions.