Zirchrom

ZirChrom HPLC Columns

Unique Selectivity & Extreme Stability

ZirChrom HPLC columns use advanced porous zirconia particle technology to deliver exceptional stability under extreme pH, temperature, and solvent conditions—far beyond the limits of traditional silica columns. This rugged chemistry makes ZirChrom ideal for demanding separations where long lifetime, low bleed, and highly reproducible retention are essential.

Each column is built from uniform 3 µm zirconia spheres produced through controlled polymerization and sintered at temperatures above 900 °C. The resulting monoclinic crystalline structure offers unmatched resistance to hydrolysis, aggressive mobile phases, and thermal stress—ensuring consistent performance across the most challenging analytical environments.

ZirChrom’s portfolio supports reversed-phase, ion-exchange, HILIC, and mixed-mode workflows, while the ProTain™ In-Line Protein Removal System enhances biological applications by removing proteins before they reach the analytical column—preserving column integrity and extending operational lifetime.

Chrom Tech proudly supplies the complete ZirChrom zirconia-based column family, helping laboratories achieve durable, high-selectivity separations for pharmaceutical, environmental, and bioanalytical methods. For expert product selection support, contact Chrom Tech .

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Key Definitions ▸
Zirconia Stationary Phase
A highly stable chromatographic support made from porous zirconia particles, offering extreme pH (1–14) and thermal stability far beyond conventional silica columns.
Lewis Acid–Base Interactions
A dominant retention mechanism on zirconia surfaces, allowing selectivity to be tuned using strong Lewis bases such as fluoride, phosphate, or hydroxide in the mobile phase.
PBD (Polybutadiene-Coated Zirconia)
A reversed-phase–like zirconia chemistry offering excellent peak shapes for basic compounds, broad pH stability, and reduced hydrophobicity compared to C18-silica.
Carbon-Clad (CARB) Zirconia
A carbon-bonded zirconia phase delivering unique shape-selective retention, ideal for diastereomers, isomers, and compounds unresolved on ODS phases.
Anion-Exchange Zirconia (WAX & SAX)
Cross-linked polyethyleneimine-coated zirconia phases used for weak or strong anion-exchange, ideal for nucleotides, oligonucleotides, amino acids, peptides, and inorganic anions.
Frequently Asked Questions ▸
How do zirconia-based HPLC columns differ from silica-based columns?
Zirconia columns offer extreme pH stability (1–14), high thermal resistance, and complete resistance to hydrolysis—providing dramatically longer lifetimes and unique Lewis-acid/Lewis-base interactions that silica columns cannot replicate.
What types of applications benefit most from zirconia columns?
ZirChrom columns are ideal for bioanalytical, pharmaceutical, environmental, and high-pH separations, especially when working with acidic, basic, or thermally sensitive analytes that require rugged stability and enhanced selectivity.
How does ProTain improve protein-containing sample analyses?
The ProTain in-line system selectively removes proteins before they reach the analytical column, reducing matrix interference, preventing fouling, and significantly extending column lifetime—ideal for biological workflows.