Posted by Chrom Tech on 15th Oct 2025
The Different 96 Well Plate Bottom Shapes
Understanding 96-Well Plate Designs and Applications
Chrom Tech’s 96-well plates are a cornerstone in high-throughput chromatography and mass spectrometry workflows, enabling automation from sample preparation through analysis. All Chrom Tech 96-well plates conform to the ANSI/SLAS standard base and footprint, ensuring seamless compatibility with leading instruments and robotic systems. Whether you’re using our 2 mL deep well plates or shallow round well plates, you can trust in consistent performance and precise dimensions that meet industry standards.
Variations in Well Plate Geometry
While all 96-well plates share the same footprint, they differ in several key features that influence their performance and compatibility with different workflows:
- Well geometry (round, square, or V-bottom formats)
- Plate height, which determines the working volume
- Internal diameter of wells, influencing capacity and evaporation rates
- Underside bracing, which affects compatibility with magnetic platforms
- Construction material (polypropylene, polystyrene, or glass)
- Rimmed or rimless designs for sealing film compatibility and contamination prevention
- Bottom shape—U, V, flat, or pyramid—for optimized liquid recovery or particle collection
96-Well Deep Round “Common Wall” Plates
Chrom Tech’s deep round common wall plates are precision-molded from virgin polypropylene to minimize extractables. With a 2.075 mL maximum volume and a 1.85 mL working volume, they provide the highest capacity in their class while maintaining a compact 45 mm height. The “common wall” design ensures optimal use of well volume while maintaining ANSI/SLAS footprint compliance, ideal for high-throughput sample storage or processing.
96-Well Shallow Round Plates
Chrom Tech’s shallow well plates are available in 350 µL, 270 µL, and 220 µL capacities, with flat, round, or V-bottom options for diverse applications in cell biology, drug discovery, screening, and genomics. These plates are excellent for compound storage, sample collection, and culture applications requiring smaller working volumes.
96-Well Low Profile Round Plates
Our low-profile 1.1 mL round plates offer a reduced height of just 27 mm, maximizing storage and stacking efficiency. Manufactured from extractable-free polypropylene, they ensure purity for sensitive analytical and biological assays. An optional thermoplastic elastomer anti-evaporation cap mat provides excellent sealing during long-term storage or thermal cycling.
96-Well Assay Plates
For Absorbance, Fluorescence, Luminescence, and Scintillation
Chrom Tech’s assay plates are optimized for a variety of optical applications. The Porvair Krystal series features clear, glass, or solid-bottom designs for precise optical readings. Our clear polystyrene plates feature 0.4 mm-thick bases molded from high-clarity materials to ensure artifact-free imaging. For advanced confocal readers, the Porvair glass-bottom plates offer superior flatness (±15 µm) and optical clarity, using 175 µm borosilicate glass for crisp, distortion-free imaging.
96 Deep Well Microplate and Accessories for Thermo Scientific™ KingFisher™ Systems
Chrom Tech supplies Porvair Sciences consumables engineered for full compatibility with Thermo Scientific™ KingFisher™ systems—including deep well plates, elution plates, and magnetic comb tips. Made from medical-grade polypropylene, these components minimize leachables and ensure high recovery of nucleic acids and proteins.
Deep Well Microplate
- 2.2 mL, 96-well deep plate
- Working volume: 50 µL–1000 µL
- V-bottom for efficient liquid recovery
- Low extractables; resistant to chemicals and temperature
- DNase/RNase/human DNA-free
Magnetic Comb Tips
- Compatible with KingFisher™ Flex, Duo Prime, and Presto instruments
- Disposable to prevent cross-contamination
- Designed for optimal magnetic bead recovery
- Low-affinity binding polypropylene construction
- DNase/RNase/human DNA-free
Elution Plate
- 200 µL capacity, V-bottom wells
- High recovery and purity
- DNase/RNase/human DNA-free



Compatibility and Performance
- Compatible with Thermo Scientific™ KingFisher™ Purification Systems (Flex, Duo Prime, Apex, and Presto)
- Compatible with Ultraseal™ Pro (#500290) and eSeal™ (#CT-ESEAL) systems
- Ideal for DNA/RNA and protein purification, immunoprecipitation, and ChIP assays
Engineered for Efficiency
Each V-shaped bottom well is designed for perfect alignment with magnetic tips, maximizing sample recovery, mixing efficiency, and throughput. Features include:
- Alphanumeric grid for accurate sample tracking
- Raised rims for heat sealing
- Autoclavable and chemically resistant construction
- ANSI/SLAS standard footprint
Summary
With multiple formats, materials, and capacities available, Chrom Tech’s 96-well plates provide flexibility and reliability for any high-throughput or analytical workflow. Many options are in stock for same-day shipment. Contact Chrom Tech to find the best well plate solution for your application.
Frequently Asked Questions About 96-Well Plates
Do Chrom Tech’s 96-well plates meet ANSI/SLAS standards?
Yes. All Chrom Tech 96-well plates conform to ANSI/SLAS footprint standards for full compatibility with automated liquid handling systems and analytical instruments.
What material is best for minimizing extractables?
Chrom Tech’s virgin polypropylene plates provide the lowest extractables and leachables, ideal for sensitive LC-MS and biomolecular workflows.
Are these plates compatible with KingFisher™ instruments?
Yes. Chrom Tech offers deep well plates, elution plates, and magnetic combs specifically designed for Thermo Scientific™ KingFisher™ systems.