The Different 96 Well Plate Bottom Shapes

96-well plates are commonly used in high-throughput chromatography mass spectrometry labs, enabling automation from sample preparation to final analysis. Due to the wide range of applications and the various instruments they must be compatible with, all Chrom Tech 96-well plates adhere to the ANSI/SLAS standard base and footprint. Whether you're using our 96 deep well plates with 2mL capacity or the 96-well shallow round well plates, you can be confident that they meet the ANSI/SLAS standard, regardless of the well dimensions.

While all 96-well plates share the same footprint, there are other variations in their geometries:

• Well plate geometry, such as round or square well formats
• Overall height of the plate, which affects the volume in each well
• Internal diameter of the wells, which also influences volume
• Underside bracing of the plate, which determines whether it can sit on a magnet
• Material of construction, such as polypropylene, polystyrene, or glass
• Rimmed or rimless tops for use with appropriate sealing films. Raised rims enhance sealing with heat-sealing systems and prevent cross-contamination
• Well bottom shapes: U-shape, round, pyramid, V-bottom, or flat. Round and V-bottom wells are ideal for efficient liquid removal and particulate collection.

96-Well Deep Round ‘Common Wall’

Chrom Tech’s revolutionary plates are made in virgin polypropylene to minimize extractables. Maximum volume is 2.075 ml per well and working volume is a useful 1.85 ml, more than any other comparable ‘2 ml Round Well’ plate in this class. The ‘common wall’ design allows the highest possible volume to be used whilst maintaining an overall height of just 45 mm, complete with ANSI/SLAS standard base and footprint. Extra working volume is thus assured in a convenient round bottom, round well format.

96-Well Shallow Round Well

Chrom Tech offers a number of storage/collection plates to help in the fields of cell biology, molecular biology, drug discovery, combinatorial chemistry, screening, and genomics. We offer three plates with well capacities of 350µl, 270µl, and 220µl. They have flat-, round- or V-bottoms and can be used for compound storage and culturing.

96-Well Round Low Profile

A new introduction to the Porvair range is a low profile 1.1ml 96-well round plate with a height of just 27mm. This allows you to store or stack more plates in a given space. The plates are made with extractable-free polypropylene for excellent results. A specific anti-evaporation cap mat is also available for this plate, which is manufactured from thermoplastic elastomer.

96 Well Assay Plates

Designed for Absorbance, Fluorescence, Luminescence, and Scintillation Applications

Chrom Tech offers a range of clear polystyrene bottom, glass bottom, and solid bottom assay plates that are specifically designed for absorbance, fluorescence, luminescence, and scintillation applications. Porvair Krystal series clear bottom plates are now recognized as industry-leading tools for Pharma drug discovery and screening. Our standard clear polystyrene bottom plates have bases just 0.4mm thick and are molded from highly polished tools to reduce flow lines, artefacts, and scratches—guaranteeing good results with all types of bottom-reading instruments. For the more modern, demanding, confocal readers, Porvair Glass Bottom plates have been shown by leading research institutes to be unsurpassed for flatness, linearity, and optical clarity. Our sparkling pure borosilicate glass is just 175µm thick with a flatness tolerance across the plate of +/- 15µm ensuring superb crisp images every time.

96 Deep Well Microplate, Combs, and Elution Plate for Thermo Scientific™ KingFisher™ Systems

Designed for a perfect fit, the Porvair Sciences 96 deep well plate, 96 well elution plate, and 96 well magnetic combs tips are specifically made to be fully compatible with the Thermo Scientific™ KingFisher™ range of purification systems. These consumables are made from medical-grade polypropylene to ensure low affinity binding of biomolecules and low leachables and extractables throughout the extraction and purification workflow. This maximizes the yield and quality of isolated proteins and nucleic acids from samples and improves assay performance when used in conjunction with KingFisher™ Flex, Duo Prime, and Presto instruments.

Deep Well Microplate for Thermo Scientific™ KingFisher™ Systems

• 2.2 ml, 96 deep well plate
• Working volume 50 μl–1000 μl
• V-bottom for maximum liquid uptake
• Low leachables and extractables
• High chemical and temperature resistance
• Free from DNase, RNase, and human DNA

Magnetic Comb Tips for Thermo Scientific™ KingFisher™ Systems

• Fully compatible for easy and secure insertion of 96 well magnetic probes
• Disposable tips prevent contamination
• Designed for high-quality recovery of magnetic beads
• Made from polypropylene for low affinity binding of biomolecules
• Free from DNase, RNase, human DNA

Elution Plate for Thermo Scientific™ KingFisher™ Systems

• 200 μl, 96 well microtiter plate
• V-bottom for maximum liquid uptake
• Free from DNase, RNase, human DNA

Compatible Instruments

• Thermo Scientific™ KingFisher™ Purification System (Duo Prime, Flex, Apex and Presto)
• Autosampler Instruments
• Ultraseal™ Pro (Product # 500290) eSeal (Product # CT-ESEAL)

Low Affinity Binding Microplates for Sample Purification

The deep well plates are manufactured using virgin polypropylene for the lowest leachables and extractables that are free from DNase and RNase. This allows samples to be purified without the risk of contamination or interference during magnetic particle processing. They’re ideal for low abundant biomolecules or high sensitivity assays.

The Versatile Microplate Is Suitable For:

• DNA and RNA purification
• Protein purification
• Protein immunoprecipitation and Co-IP
• ChIP Assays

Designed for Compatibility and Efficiency

The V-shaped bottom well supports the specialized magnetic tips of all KingFisher™ Instruments with a perfect fit and maximizes liquid sample collection, mixing, and uptake during the purification process. From sample collection and mixing to purification, the Porvair Sciences 96 deep well plate ensures reproducible purification of cells, proteins, and nucleic acids from a wide range of samples.

• Alphanumeric reference grid for easy tracking and dispensing of samples
• Perfect for short- and long-term sample storage
• Raised rims for heat sealing
• Autoclavable
• ANSI/SLS standard footprint

Summary

There are a lot of options for well plate shapes and geometries, and we keep quite a few 96 well plates in stock for same-day shipment. Please reach out to us at Chrom Tech to see which plate is best for your application.