The Different 96 Well Plate Bottom Shapes

Understanding 96-Well Plate Designs and Applications

Chrom Tech’s 96-well plates are a cornerstone in high-throughput chromatography and mass spectrometry workflows, enabling automation from sample preparation through analysis. All Chrom Tech 96-well plates conform to the ANSI/SLAS standard base and footprint, ensuring seamless compatibility with leading instruments and robotic systems. Whether you’re using our 2 mL deep well plates or shallow round well plates, you can trust in consistent performance and precise dimensions that meet industry standards.

Variations in Well Plate Geometry

While all 96-well plates share the same footprint, they differ in several key features that influence their performance and compatibility with different workflows:

• Well geometry (round, square, or V-bottom formats)
• Plate height, which determines the working volume
• Internal diameter of wells, influencing capacity and evaporation rates
• Underside bracing, which affects compatibility with magnetic platforms
• Construction material (polypropylene, polystyrene, or glass)
• Rimmed or rimless designs for sealing film compatibility and contamination prevention
• Bottom shape—U, V, flat, or pyramid—for optimized liquid recovery or particle collection

96-Well Deep Round “Common Wall” Plates

Chrom Tech’s deep round common wall plates are precision-molded from virgin polypropylene to minimize extractables. With a 2.075 mL maximum volume and a 1.85 mL working volume, they provide the highest capacity in their class while maintaining a compact 45 mm height. The “common wall” design ensures optimal use of well volume while maintaining ANSI/SLAS footprint compliance, ideal for high-throughput sample storage or processing.

96-Well Shallow Round Plates

Chrom Tech’s shallow well plates are available in 350 µL, 270 µL, and 220 µL capacities, with flat, round, or V-bottom options for diverse applications in cell biology, drug discovery, screening, and genomics. These plates are excellent for compound storage, sample collection, and culture applications requiring smaller working volumes.

96-Well Low Profile Round Plates

Our low-profile 1.1 mL round plates offer a reduced height of just 27 mm, maximizing storage and stacking efficiency. Manufactured from extractable-free polypropylene, they ensure purity for sensitive analytical and biological assays. An optional thermoplastic elastomer anti-evaporation cap mat provides excellent sealing during long-term storage or thermal cycling.

96-Well Assay Plates

For Absorbance, Fluorescence, Luminescence, and Scintillation

Chrom Tech’s assay plates are optimized for a variety of optical applications. The Porvair Krystal series features clear, glass, or solid-bottom designs for precise optical readings. Our clear polystyrene plates feature 0.4 mm-thick bases molded from high-clarity materials to ensure artifact-free imaging. For advanced confocal readers, the Porvair glass-bottom plates offer superior flatness (±15 µm) and optical clarity, using 175 µm borosilicate glass for crisp, distortion-free imaging.

96 Deep Well Microplate and Accessories for Thermo Scientific™ KingFisher™ Systems

Chrom Tech supplies Porvair Sciences consumables engineered for full compatibility with Thermo Scientific™ KingFisher™ systems—including deep well plates, elution plates, and magnetic comb tips. Made from medical-grade polypropylene, these components minimize leachables and ensure high recovery of nucleic acids and proteins.

Deep Well Microplate

• 2.2 mL, 96-well deep plate
• Working volume: 50 µL–1000 µL
• V-bottom for efficient liquid recovery
• Low extractables; resistant to chemicals and temperature
• DNase/RNase/human DNA-free

Magnetic Comb Tips

• Compatible with KingFisher™ Flex, Duo Prime, and Presto instruments
• Disposable to prevent cross-contamination
• Designed for optimal magnetic bead recovery
• Low-affinity binding polypropylene construction
• DNase/RNase/human DNA-free

Elution Plate

• 200 µL capacity, V-bottom wells
• High recovery and purity
• DNase/RNase/human DNA-free

Compatibility and Performance

• Compatible with Thermo Scientific™ KingFisher™ Purification Systems (Flex, Duo Prime, Apex, and Presto)
• Compatible with Ultraseal™ Pro (#500290) and eSeal™ (#CT-ESEAL) systems
• Ideal for DNA/RNA and protein purification, immunoprecipitation, and ChIP assays

Engineered for Efficiency

Each V-shaped bottom well is designed for perfect alignment with magnetic tips, maximizing sample recovery, mixing efficiency, and throughput. Features include:

• Alphanumeric grid for accurate sample tracking
• Raised rims for heat sealing
• Autoclavable and chemically resistant construction
• ANSI/SLAS standard footprint

Summary

With multiple formats, materials, and capacities available, Chrom Tech’s 96-well plates provide flexibility and reliability for any high-throughput or analytical workflow. Many options are in stock for same-day shipment. Contact Chrom Tech to find the best well plate solution for your application.