Syringe Filters

As chromatography has evolved toward higher pressures and smaller column dimensions, particle control has become critical to achieving reliable and reproducible results. Chrom Tech’s HPLC syringe filters are designed to prevent particulates from entering your system, minimizing buildup and reducing instrument downtime. Using the correct syringe filter protects sensitive components, extends column life, and maintains consistent data quality across applications.

  • Available in two standard diameters: 13 mm and 30 mm
  • Pore sizes: 0.2 µm and 0.45 µm for fine or general filtration
  • Glass fiber filters available in 1.2 µm and 0.7 µm pore sizes
  • Offered in seven color-coded membrane types for easy identification

Choose from a wide range of membrane materials, including Nylon, PTFE, PVDF, PES, Polypropylene (PP), Cellulose Acetate (CA), and Glass Fiber (GF)—each engineered for specific chemical compatibilities and performance needs. Learn more about selection in our guide on how to choose a syringe filter , or contact sales@chromtech.com for assistance.

All available membranes can also be purchased as sheets for custom applications.

SKU DESCRIPTION
PORE SIZE
DIAMETER
MEMBRANE
GF PREFILTER
SYRINGE FILTERS
F13-CA020 CA Syringe Filter 0.2 µm 13 mm CA —
F13-CA045 CA Syringe Filter 0.45 µm 13 mm CA —
F13-NY020 Nylon Syringe Filter 0.2 µm 13 mm Nylon —
F13-NY045 Nylon Syringe Filter 0.45 µm 13 mm Nylon —
F13-PP020 PP Syringe Filter 0.2 µm 13 mm PP —
F13-PP045 PP Syringe Filter 0.45 µm 13 mm PP —
F13-PS020 PES Syringe Filter 0.2 µm 13 mm PES —
F13-PS045 PES Syringe Filter 0.45 µm 13 mm PES —
F13-PT020 PTFE Syringe Filter 0.2 µm 13 mm PTFE —
F13-PT045 PTFE Syringe Filter 0.45 µm 13 mm PTFE —
F13-PV020 PVDF Syringe Filter 0.2 µm 13 mm PVDF —
F13-PV045 PVDF Syringe Filter 0.45 µm 13 mm PVDF —
F13-GF070 Glass Fiber Syringe Filter 0.7 µm 13 mm Glass Fiber —
F13-GF120 Glass Fiber Syringe Filter 1.2 µm 13 mm Glass Fiber —
F30-CA020 CA Syringe Filter 0.2 µm 30 mm CA —
F30-CA045 CA Syringe Filter 0.45 µm 30 mm CA —
F30-GF070 Glass Fiber Syringe Filter 0.7 µm 30 mm Glass Fiber —
F30-GF120 Glass Fiber Syringe Filter 1.2 µm 30 mm Glass Fiber —
F30-NY020 Nylon Syringe Filter 0.2 µm 30 mm Nylon —
F30-NY045 Nylon Syringe Filter 0.45 µm 30 mm Nylon —
F30-PP020 PP Syringe Filter 0.2 µm 30 mm PP —
F30-PP045 PP Syringe Filter 0.45 µm 30 mm PP —
F30-PS020 PES Syringe Filter 0.2 µm 30 mm PES —
F30-PS045 PES Syringe Filter 0.45 µm 30 mm PES —
F30-PT020 PTFE Syringe Filter 0.2 µm 30 mm PTFE —
F30-PT045 PTFE Syringe Filter 0.45 µm 30 mm PTFE —
F30-PV020 PVDF Syringe Filter 0.2 µm 30 mm PVDF —
F30-PV045 PVDF Syringe Filter 0.45 µm 30 mm PVDF —
F25-NY020GF Nylon Syringe Filter, GF Prefilter 0.2 µm 25 mm Nylon Yes
F13-NY020GF Nylon Syringe Filter, GF Prefilter 0.2 µm 13 mm Nylon Yes
Membrane Selection Guide ▶
Membrane Aqueous Organic Low Protein
Binding 		Application
Nylon ● ●   General laboratory and HPLC sample filtration prior to injection. Solvent resistant with low extractables; not ideal when protein recovery is critical.
PTFE   ●   Hydrophobic and chemically resistant to most solvents, acids, and bases. Low extractables; can block water vapor (useful for transducer protection).
PVDF ● ● ● Solvent resistant membrane with low UV-adsorbing extractables. Used for HPLC sample filtration and general biological filtration; low protein binding.
PES ● ● ● Mechanically strong with low inorganic extractables. Typical uses include ion chromatography, tissue culture filtration, and filtration of proteins and nucleic acids.
Cellulose Acetate ●   ● Very low protein binding (often less than PVDF) and ideal for aqueous samples. Excellent when maximum protein recovery in filtrate is critical.
PP ● ●   Hydrophilic membrane with broad chemical compatibility to many organic solvents. A strong choice for HPLC sample filtration in protein analysis workflows.
RC ● ● ● Regenerated Cellulose is hydrophilic, solvent resistant, and low protein binding. Ideal for removing particulates from HPLC samples prior to injection; low water extractables.
NC ●     Nitrocellulose (mixed esters) filters are hydrophilic and made from cellulose nitrate/cellulose acetate blends. Fast flow rates due to a uniform membrane structure.
Legend: ● = compatible / recommended.
Key Definitions ▶
Syringe Filter
A disposable filtration device attached to a syringe used to remove particulates from liquid samples prior to HPLC or analytical injection, protecting columns and instruments while improving reproducibility.
Pore Size
The diameter of membrane openings (commonly 0.2 µm or 0.45 µm) that determines particle retention. Smaller pore sizes provide finer filtration for sensitive chromatography systems.
Membrane Material
The filter media inside a syringe filter—such as Nylon, PTFE, PVDF, PES, Polypropylene (PP), Cellulose Acetate (CA), or Glass Fiber (GF)—selected based on solvent compatibility, protein binding, and analytical requirements.
Filter Diameter
The physical size of the syringe filter housing—typically 13 mm or 30 mm—which affects sample throughput and flow rate during filtration.
Glass Fiber Prefilter
A depth-style filter layer (commonly 0.7 µm or 1.2 µm) used to remove larger particulates before membrane filtration, extending filter life when processing heavily loaded samples.
Frequently Asked Questions ▶
Why should I filter samples before HPLC injection?
Syringe filtration removes particulates that can clog columns, damage injector valves, and increase system backpressure. Proper filtration extends column life and helps maintain consistent, reproducible chromatographic results.
Should I use a 0.2 µm or 0.45 µm syringe filter?
Use 0.2 µm filters for UHPLC or sensitive analytical systems requiring fine particulate removal. Use 0.45 µm filters for general HPLC sample prep where higher flow rates and faster filtration are preferred.
How do I choose the correct membrane material?
Membrane selection depends on solvent compatibility and protein binding. Nylon and PES are common for aqueous samples, PTFE is ideal for aggressive organic solvents, and PVDF or Cellulose Acetate are preferred for low protein binding applications.
What filter diameter should I use?
13 mm syringe filters are best for small sample volumes, while 30 mm filters provide higher throughput and are better suited for larger volumes or viscous samples.
When should I use glass fiber syringe filters?
Glass fiber filters (0.7 µm or 1.2 µm) are ideal for heavily particulate samples and can be used as prefilters to prevent premature clogging of finer membrane syringe filters.