Reversed-Phase

Hamilton Reversed-Phase HPLC Columns

Hamilton’s reversed-phase HPLC columns are engineered to deliver exceptional inertness, longevity, and batch-to-batch consistency across a wide range of analytical workflows. By merging the strengths of both silica-based and polymeric stationary phases, these columns provide robust performance for laboratories seeking reliable separations of small molecules, peptides, pharmaceuticals, and complex organic mixtures.

Hamilton offers an extensive portfolio that includes four polymeric and two silica-based reversed-phase packing materials, each optimized for specific selectivity, retention, and chemical stability requirements. The polymeric phases provide outstanding resistance to high pH, aggressive mobile phases, and repeated cleaning cycles—ideal for high-throughput laboratories and challenging sample matrices. The silica-based phases deliver high efficiency and sharp peak shapes for traditional reversed-phase workflows, ensuring analytical precision even with demanding separations.

Together, these reversed-phase chemistries allow analysts to fine-tune methods for maximum resolution, reproducibility, and sensitivity. Whether you need rugged polymeric durability or classic silica-based selectivity, Hamilton’s HPLC columns offer reliable performance suitable for pharmaceutical, environmental, clinical, food and beverage, and research applications.

Hamilton Reversed-Phase HPLC Columns — Specifications

Packing Name Support Material Pore Size pH Range Temp (°C) Max Pressure Applications Typical Analytes
HxSil C18 Silica, end capped 110 Å 1.5–10.0 5–60 6,000 PSI General reversed-phase separations of hydrophobic organic compounds Small molecules, pharmaceuticals, peptides
HxSil C8 Silica, end capped 110 Å 1.5–10.0 5–60 6,000 PSI Reduced hydrophobic retention for faster reversed-phase methods Drugs, metabolites, moderately hydrophobic compounds
PRP-C18 C18 bonded to PSDVB* 100 Å 1–13 5–85 5,000 PSI Organic compounds: small molecules (< 2,000 MW), pharmaceuticals, steroids, halides, vitamins, amino acid analysis, herbicides Peptides, DNA, RNA, oligonucleotides, nucleotides, vitamins, steroids, herbicides, pharmaceutical compounds
PRP-1 PSDVB* 100 Å 1–13 5–85 5,000 PSI Organic compounds: small molecules (< 2,000 MW), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides Polycyclic aromatic hydrocarbons (PAH), ionizable organic compounds, steroids, peptide fragments
PRP-3 PSDVB* 300 Å 1–13 5–85 5,000 PSI Organic compounds: large molecules (> 2,000 MW), peptides, proteins, protein digests, protected and de-protected oligonucleotides, nucleic acids Globular proteins, albumins, antibody fragments, tryptic digests, DNA, RNA oligomers, synthetic high-MW polymers
PRP-h5 Fluorinated PSDVB* 300 Å 1–13 5–85 5,000 PSI Organic compounds: macromolecules (> 2,000 MW), pharmaceuticals, protein digests, tryptic digests, proteomics Oligonucleotides, angiotensin, apo-transferrin, apomyoglobin (equine), carbonic anhydrase, cytochrome C, myoglobin, ribonuclease A

*PSDVB = Poly(styrene-divinylbenzene)

Hamilton HxSil C18 & HxSil C8 Columns — Silica Packing

Hamilton HxSil reversed-phase columns are engineered with high-purity, well end-capped silica to deliver consistent retention, excellent reproducibility, and long-term durability in analytical HPLC workflows.

HxSil C18 columns provide enhanced hydrophobic retention compared to many conventional C18 phases, improving separation of compounds that are often poorly retained on standard columns. This makes HxSil C18 an ideal choice for challenging analytes requiring increased interaction with the stationary phase while maintaining reliable retention time stability.

HxSil C8 columns offer the same end-capped silica chemistry with reduced alkyl chain length, delivering excellent selectivity for complex sample mixtures while producing shorter retention times than C18. HxSil C8 is commonly selected when faster analyses are needed without sacrificing chromatographic resolution.

Key Performance Benefits

  • High-purity, end-capped silica for reproducible retention
  • Improved durability for routine analytical operation
  • HxSil C18 for enhanced retention of weakly retained compounds
  • HxSil C8 for faster separations with excellent selectivity

Both HxSil C18 and C8 phases are well suited for pharmaceutical, environmental, and research applications requiring dependable reversed-phase performance across a wide range of mobile phase conditions.

HxSil C18 HxSil C8
Size (mm) 3 µm 5 µm 3 µm 5 µm
4.6 x 250 SS — 79869 — —
4.6 x 150 SS — 79868 — —
4.6 x 100 SS — — — 79101
2.1 x 100 SS — — — 79109
2.1 x 50 SS 79889 — 79117 79107
2.1 x 33 SS — — 79116 —
Guard Columns & Accessories
Analytical Guard Column7929979298
Analytical Guard Cartridge Holder SS3290832908
Analytical Repl Cartridges SS (5/pk)7945279451
Analytical Starter Kit¹ SS7945979458
Prep/Semiprep Guard Column7992379922
Prep/Semiprep Guard Cartridge Holder SS5095-015095-01
Prep/Semiprep Repl Cartridges SS (2/pk)7913879136
Prep/Semiprep Starter Kit² SS7913779135
1) Analytical Starter Kit includes 1 holder, 2 cartridges
2) Prep/Semiprep Starter Kit includes 1 holder, 1 cartridge

Hamilton PRP-C18 Columns — Polymer Packing

Hamilton PRP-C18 columns are engineered with polymer-based stationary phase chemistry to deliver high-efficiency separations across the full pH range while providing extended column lifetime and exceptional loading capacity. Designed for demanding analytical workflows, PRP-C18 enables reliable performance where traditional silica-based phases may degrade.

This robust polymer packing supports superior resolution for oligonucleotides and biomolecules while maintaining strong retention for small organic compounds. High loading capacity allows increased sample throughput, improving productivity and reducing overall analysis cost.

Key Performance Benefits

  • Superior resolution for purer oligos and complex biomolecules
  • Extended column lifetime for increased laboratory productivity
  • High loading capacity for time and cost savings
  • High-efficiency separations across the full pH range

Typical Analytes

Peptides, DNA, RNA, oligonucleotides, nucleotides, vitamins, steroids, herbicides, and pharmaceutical compounds.

Applications

Optimized for organic compounds and small molecules (<2,000 MW), PRP-C18 columns are widely used in pharmaceutical analysis, steroid profiling, halide separations, vitamin analysis, amino acid characterization, and herbicide detection.

Hamilton PRP-C18 polymer columns provide dependable reversed-phase performance for laboratories requiring chemical stability, high sample loading, and reproducible results in both routine and specialized analytical environments.

Size (mm) 5 µm 12–20 µm
21.2 x 50 SS—79678
4.6 x 250 SS79677—
4.6 x 250 PEEK79684—
4.6 x 150 SS79676—
4.6 x 150 PEEK79683—
4.6 x 50 SS79675—
4.6 x 50 PEEK79682—
2.1 x 250 SS79674—
2.1 x 250 PEEK79681—
2.1 x 150 SS79673—
2.1 x 150 PEEK79680—
2.1 x 50 SS79672—
2.1 x 50 PEEK79679—
Guard Columns & Accessories
Analytical Guard Column79291
Analytical Guard Cartridge Holder SS32908
Analytical Guard Cartridge Holder PEEK79477
Analytical Repl Cartridges SS (5/pk)79686
Analytical Repl Cartridges PEEK (5/pk)79688
Analytical Starter Kit¹ SS79685
Analytical Starter Kit¹ PEEK79687
Prep/Semiprep Guard Column79918
Prep/Semiprep Guard Cartridge Holder SS5095-01
Prep/Semiprep Repl Cartridges SS (2/pk)79690
Prep/Semiprep Starter Kit² SS79689
1) Analytical Starter Kit includes 1 holder, 2 cartridges
2) Prep/Semiprep Starter Kit includes 1 holder, 1 cartridge

Hamilton PRP-1 Columns — Polymer Packing

Hamilton PRP-1 columns are designed to maximize sample recovery during purification and quantitation—especially for labile or reactive compounds that can irreversibly bind to silica-based C8/C18 phases. Because Hamilton PRP polymer supports are made entirely of poly(styrene-divinylbenzene) (PSDVB), they do not contain silanol groups that can cause analyte loss.

PRP-1 is particularly valuable for protected oligonucleotides and other sensitive analytes where recovery is critical. In many applications, PRP-1 can deliver 95% or greater recovery of the same sample that may only partially recover on traditional C18 columns—supporting higher confidence quantitation and improved workflow efficiency.

Unlike silica-based C8/C18 columns, PRP-1 has no stationary phase coating. With no coating to hydrolyze, the column maintains performance characteristics longer than many conventional reversed-phase columns, helping laboratories reduce downtime and replacement frequency.

Typical Analytes

Polycyclic aromatic hydrocarbons (PAH), ionizable organic compounds, steroids, and peptide fragments.

Applications

Well suited for small organic compounds (<2,000 MW), pharmaceutical analysis, steroid profiling, nucleic acid separations, vitamin analysis, and herbicide detection—especially when enhanced recovery and long-term stability are required.

Size (mm) 5 µm 7 µm 10 µm 12–20 µm
50.0 x 250 SS — — 79567 79493
30.0 x 250 SS — — — 79229
21.2 x 250 SS — 79352 79478 79428
21.2 x 75 SS 79154 — — —
10.0 x 250 SS — 79531 79496 —
10.0 x 100 SS 79355 — 79499 —
10.0 x 50 SS — 79367 — —
7.0 x 305 SS 79795 — 79426 —
7.0 x 100 SS — — 79495 —
4.6 x 250 SS 79571 79380 79381 —
4.6 x 150 SS 79423 — 79351 —
4.6 x 100 SS 79558 — — —
4.1 x 250 SS 79820 79422 79427 —
4.1 x 150 SS 79444 79529 79425 —
4.1 x 100 SS 79479 — — —
4.1 x 50 SS 79443 — — —
2.1 x 150 SS 79366 — — —
2.1 x 100 SS 79790 — — —
1.0 x 150 SS 79753 — — —
1.0 x 50 SS — 79755 — —
Guard Columns & Accessories
Analytical Guard Column79286
Analytical Guard Cartridge Holder SS32908
Analytical Guard Cartridge Holder PEEK79477
Analytical Repl Cartridges SS (5/pk)79445
Analytical Repl Cartridges PEEK (5/pk)79318
Analytical Starter Kit¹ SS79447
Analytical Starter Kit¹ PEEK79317
Prep/Semiprep Guard Column79912
Prep/Semiprep Guard Cartridge Holder SS5095-01
Prep/Semiprep Repl Cartridges SS (2/pk)79122
Prep/Semiprep Starter Kit² SS79121
1) Analytical Starter Kit includes 1 holder, 2 cartridges
2) Prep/Semiprep Starter Kit includes 1 holder, 1 cartridge

Hamilton PRP-3 & PRP-h5 Columns — Polymer Packing

Hamilton PRP-3 is a polymeric reversed-phase HPLC column designed for the purification and isolation of proteins and peptides with excellent recovery (>90%). Built on an inert poly(styrene-divinylbenzene) (PSDVB) support, PRP-3 eliminates silanol-driven adsorption that can irreversibly bind biomolecules and reduce yield.

PRP-3 is based on PRP-1 chemistry but uses a larger 300 Å pore size (vs. PRP-1’s 100 Å), improving accessibility for larger analytes such as proteins, digests, and high molecular weight polymers.

PRP-h5 uses the PRP-3 base with a pentafluorinated modification, increasing hydrophobic character and delivering selectivity differences compared with standard silica C18 phases. This behavior can be especially useful for halogenated solutes and other analytes where altered selectivity improves separation.

Typical Analytes

PRP-3: Globular proteins, albumins, antibody fragments, tryptic digests, DNA, RNA oligomers, synthetic high MW polymers

PRP-h5: Oligonucleotides, angiotensin, apo-transferrin, apomyoglobin (equine), carbonic anhydrase, cytochrome C, myoglobin, ribonuclease A

Applications

PRP-3: Organic compounds and large molecules (>2,000 MW), peptides, proteins, protein digests, protected and de-protected oligonucleotides, nucleic acids

PRP-h5: Organic compounds and macromolecules (>2,000 MW), pharmaceuticals, protein digests, tryptic digests, and proteomics workflows where enhanced hydrophobicity and altered selectivity are beneficial

PRP-3 PRP-h5
Size (mm) 10 µm 12–20 µm 5 µm
21.2 x 250 SS—79469—
21.2 x 100 SS—79186—
10.0 x 250 SS79526——
10.0 x 150 SS——79274
10.0 x 100 SS——79263
7.0 x 305 SS79468——
4.6 x 250 SS79574—79273
4.6 x 150 SS79382—79272
4.6 x 100 SS——79262
4.6 x 50 SS——79261
4.1 x 250 SS79794——
4.1 x 150 SS79466——
2.1 x 150 SS79392—79271
2.1 x 100 SS——79270
Guard Columns & Accessories
Analytical Guard Column7929579296
Analytical Guard Cartridge Holder SS3290832908
Analytical Guard Cartridge Holder PEEK79477—
Analytical Repl Cartridges SS (5/pk)7945479268
Analytical Repl Cartridges PEEK (5/pk)79395—
Analytical Starter Kit¹ SS7946179267
Analytical Starter Kit¹ PEEK79393—
Prep/Semiprep Guard Column7992079921
Prep/Semiprep Guard Cartridge Holder SS5095-015095-01
Prep/Semiprep Repl Cartridges SS (2/pk)7912479278
Prep/Semiprep Starter Kit² SS7912379277
1) Analytical Starter Kit includes 1 holder, 2 cartridges
2) Prep/Semiprep Starter Kit includes 1 holder, 1 cartridge
Key Definitions ▸
Reversed-Phase Chromatography
A chromatographic mode where the stationary phase is nonpolar and the mobile phase is relatively polar. Commonly used for separating organic molecules, pharmaceuticals, peptides, and other hydrophobic analytes.
Silica-Based Columns
Traditional reversed-phase columns using silica particles chemically bonded with hydrophobic ligands. They provide high efficiency and sharp peak shapes but have limited pH stability compared to polymeric phases.
Polymeric Reversed-Phase Packing
Stationary phases made from cross-linked polymeric substrates that offer superior chemical stability, extended pH range, and resistance to aggressive cleaning solvents—ideal for demanding or repetitive workflows.
Inertness
A measure of how chemically non-reactive a column’s stationary phase is. High inertness ensures minimal analyte adsorption, improved peak shape, and accurate quantitation for sensitive or reactive compounds.
Stationary Phase Selectivity
The specific ways a column’s bonded phase interacts with analytes—determining retention, resolution, and separation performance. Different Hamilton packing materials provide tailored selectivity profiles for various reversed-phase applications.
Frequently Asked Questions ▸

What advantages do Hamilton reversed-phase columns offer over traditional silica-based columns?

Hamilton combines the ruggedness of polymeric materials with the high efficiency of silica, delivering columns that are inert, long-lasting, and suitable for a wide range of reversed-phase applications. This hybrid approach improves durability, peak shape, and reproducibility.

What types of samples are Hamilton reversed-phase columns designed for?

These columns support a broad spectrum of applications including pharmaceuticals, peptides, food and beverage components, environmental extracts, and general organic compounds—making them highly versatile in analytical laboratories.

How do I choose between Hamilton’s polymeric and silica-based reversed-phase options?

Select silica-based materials when you need very high efficiency and classical reversed-phase selectivity. Choose polymeric phases for high-pH methods, aggressive solvents, longer column lifetime, or challenging sample matrices requiring enhanced chemical stability.