Protein Crash Filter Plates

Protein Crash Filter Plates for Semi-Automation

• Enable semi-automated protein crash workflows for improved efficiency
• Accelerate analysis with high-throughput sample preparation
• Enhance reproducibility—protein precipitate is cleanly removed by filtration
• Eliminate the need for manual supernatant transfer steps

Chrom Tech’s Protein Crash Filter Plates provide a simple, rapid, and consistent solution for high-throughput protein precipitation and cleanup. The graded frit design ensures efficient removal of precipitated proteins without clogging or breakthrough—delivering clear filtrates for LC-MS or HPLC analysis.

Protein precipitation can be performed directly within the plate wells, minimizing handling steps and reducing sample loss. By integrating precipitation and filtration into one device, centrifugation and supernatant transfer steps are eliminated, improving both throughput and reproducibility.

These filter plates are fully compatible with all standard liquid handling systems and automated laboratory processors for seamless integration into semi-automated workflows.

Protein Crash Filter Plates Protocol

1. Use an acetonitrile-to-serum ratio of 3:1 (v/v).
2. Spike serum with the desired analytical standard.
3. Add the acetonitrile and serum mixture to each well.
4. Cover the plate with a cap mat and vortex thoroughly.
5. Plates may be vortexed with collection trays in place to prevent plate tips from contacting the vortexer platform.
6. Extract samples using positive pressure with Chrom Tech’s 96 Positive Pressure Processor (288-0001).