Polaris
Agilent Polaris HPLC Columns
Agilent Polaris HPLC columns are engineered specifically for high-aqueous reversed-phase separations—an essential requirement as modern drug discovery and biochemical workflows continue to shift toward increasingly polar analytes. Built on ultra-pure Type B silica with advanced silanol shielding and high-density bonding, Polaris columns deliver exceptional peak shape, stable retention, and reliable performance even under demanding low-organic conditions.
The Polaris family offers multiple polar-modified chemistries to provide enhanced retention, reduced secondary interactions, and improved selectivity for challenging polar compounds:
- Polaris C18-A — The flagship starting point when polar-modified selectivity is needed. Designed to prevent poor peak shape and phase collapse in high-aqueous mobile phases.
- Polaris C8-A — Offers lower hydrophobicity and alternate selectivity for polar samples, delivering faster run times and excellent peak shape.
- Polaris C18-Ether — Enhanced retention for polar analytes and improved separation away from the void volume; ideal for compounds inadequately resolved on standard C18.
- Polaris C8-Ether — Provides unique selectivity for hydrogen-bonding analytes and challenging compound classes.
- Polaris Amide C18 — Embedded amide functionality introduces subtle but valuable selectivity differences without the steric protection seen in conventional embedded-group phases.
From collapse-resistant pore structures to wettability-enhanced bonded phases, every aspect of the Polaris design supports robust performance in high-aqueous environments. The result is stable retention, reproducible chromatography, and superior peak shape across a wide range of polar compounds—making Polaris a trusted solution for pharmaceutical, environmental, food, and metabolomics applications requiring dependable reversed-phase separations under low-organic conditions.
For assistance selecting the right Polaris column for your workflow, contact Chrom Tech or speak with our technical support specialists.
Polar-Modified Reversed-Phase Columns
Reversed-phase materials engineered with polar functional groups to improve peak shape, retention, and stability under highly aqueous mobile phase conditions. Essential for analytes prone to early elution or poor interaction on standard alkyl phases.
Aqueous-Compatible Bonding
A bonding technology designed to prevent phase collapse in low-organic mobile phases—critical for polar compounds requiring retention under 100% aqueous or near-aqueous conditions.
Silanol Shielding
Techniques such as endcapping, embedded polar groups, or dense bonding that mask reactive silanol sites to reduce tailing—especially for basic analytes prone to secondary interactions with silica surfaces.
Alternative Selectivity
A chromatographic behavior distinct from standard C18 or C8 phases, often driven by polar groups, ether linkages, amide embedding, or aromatic modifiers—valuable when resolving isomers, polar analytes, or hydrogen-bonding species.
High-Purity Type B Silica
Low-metal, ultra-pure silica used in modern LC columns to minimize unwanted interactions, suppress tailing, and improve reproducibility across acidic, neutral, and basic compounds.
What makes Agilent Polaris columns ideal for highly aqueous mobile phases?
Polaris columns use polar-modified bonding, ultra-pure silica, and collapse-resistant pore structures to maintain retention under 100% aqueous conditions—preventing phase collapse, shifting retention, and poor wetting common in traditional reversed-phase columns.
Which Polaris phase should I choose for polar or early-eluting analytes?
Polaris C18-A and C18-Ether offer enhanced retention for polar compounds, while Polaris C8-A provides faster analysis for very polar samples. Polaris Amide C18 delivers subtle selectivity changes for analytes prone to hydrogen bonding.
Are Polaris columns suitable for drug discovery and modern polar compound libraries?
Yes. Polaris phases were specifically designed for today’s highly polar discovery compounds. They provide strong aqueous retention, minimal secondary interactions, and consistent peak shape—ideal for pharmaceutical method development and high-throughput screening.
Product List
| SKU | Name | LC Column Phase | Particle Size | Inner Diameter (ID) | Length |
|---|---|---|---|---|---|
SKU
A2000200X046
|
Name
A2000200X046 - Polaris 5 C18-A 200 x 4.6mm
|
LC Column Phase
C18-A
|
Particle Size
5 um
|
Inner Diameter (ID)
4.6 mm
|
Length
200 mm
|
SKU
A2007MG2
|
Name
A2007MG2 - Polaris 3 Amide-C18 2.0mm MetaGuard
|
LC Column Phase
|
Particle Size
3.0 um
|
Inner Diameter (ID)
|
Length
|
SKU
A2002250X046
|
Name
A2002250X046 - Polaris 10 C18-A 250 x 4.6mm
|
LC Column Phase
C18-A
|
Particle Size
10 um
|
Inner Diameter (ID)
4.6 mm
|
Length
250 mm
|
SKU
A2005MG
|
Name
A2005MG - MetaGuard 4.6mm Polaris Si-A 3u
|
LC Column Phase
Si-A
|
Particle Size
3 um
|
Inner Diameter (ID)
4.6 mm
|
Length
|
SKU
A2007MG
|
Name
A2007MG - Polaris 3 Amide-C18 4.6mm MetaGuard
|
LC Column Phase
|
Particle Size
3.0 um
|
Inner Diameter (ID)
|
Length
|
SKU
A2000150X212
|
Name
A2000150X212 - Polaris 5 C18-A 150 x 21.2mm
|
LC Column Phase
C18-A
|
Particle Size
5 um
|
Inner Diameter (ID)
21.2 mm
|
Length
150 mm
|
SKU
A2005250X046
|
Name
A2005250X046 - Polaris 3 Si 250 x 4.6mm
|
LC Column Phase
|
Particle Size
5 um
|
Inner Diameter (ID)
|
Length
250 mm
|
SKU
A2007250X046
|
Name
A2007250X046 - Polaris 3 Amide-C18 250 x 4.6mm
|
LC Column Phase
Amide-C18
|
Particle Size
3 um
|
Inner Diameter (ID)
4.6 mm
|
Length
250 mm
|
SKU
A2000150X046
|
Name
A2000150X046 - Polaris 5 C18-A 150 x 4.6mm
|
LC Column Phase
C18-A
|
Particle Size
5 um
|
Inner Diameter (ID)
4.6 mm
|
Length
150 mm
|
SKU
A2001MG
|
Name
A2001MG - MetaGuard 4.6mm Polaris 3 C18-A
|
LC Column Phase
C18-A
|
Particle Size
3 um
|
Inner Diameter (ID)
4.6 mm
|
Length
|
SKU
A2005250X020
|
Name
A2005250X020 - Polaris 3 Si 250 x 2.0mm
|
LC Column Phase
|
Particle Size
3.0 um
|
Inner Diameter (ID)
|
Length
250 mm
|
SKU
A2007250X030
|
Name
A2007250X030 - Polaris 3 Amide-C18 250 x 3.0mm
|
LC Column Phase
Amide-C18
|
Particle Size
3 um
|
Inner Diameter (ID)
3 mm
|
Length
250 mm
|