Organic Acid Analysis

Concise Separations Columns for Organic Acid Analysis

  • Wide pH stability from 0 to 14 for robust, flexible method development
  • Thermally stable at temperatures up to 90 Â°C
  • Consistent performance maintained through numerous injections with proper sample prep, clean system operation, and guard column protection
  • Isocratic separations enabled by simple dilute acid—no gradients required
  • Eliminates the need for costly organic solvents and solvent waste handling
  • Eluent acts as a self-regenerating cleaning solution without degrading column chemistry

Concise Separations organic acid columns are engineered for high-efficiency, reproducible separation of a wide variety of organic acids using simple, low-cost eluents. These columns rely on well-defined acid–base interactions within the stationary phase, enabling predictable retention across a broad pH range and delivering excellent peak shape for even highly polar, strongly retained acids.

Eluent Effect: By precisely controlling the true strength of the acidic mobile phase, analysts can fine-tune compound retention relative to each analyte’s pKa. A stronger (more acidic) eluent increases retention times and enhances the separation of compounds with closely spaced acid dissociation constants. This tunable behavior allows the column to deliver optimized selectivity without gradients or complex solvent systems, while maintaining exceptional robustness and long lifetime.

PHASE CROSS-LINKAGE IONIC FORM PARTICLE SIZE (µm) KEY SAMPLES COMMENTS
COREGEL-87H1 8 H 10 Short bed length allows for fast analysis of simple acid samples Ruggedness combined with fast analysis
COREGEL-87H3 8 H 9 Good resolution of many common organic acids High durability
COREGEL ION-300 6 H 7 Separates organic acids, alcohols and carbohydrates all on the same column Popular column. Select when high resolution is the primary concern
COREGEL-107H 10 H 8 Improved resolution for organic acids New higher cross-linked column
COREGEL ORH-801 7 H 9 Versatile column for organic acids, alcohols and carbohydrates Popular column. Provides good balance of high efficiency and ruggedness
COREGEL ORH-801FA 7 H 8 Fast analysis for fermentation monitoring, versatile column for organic acids, alcohols and carbohydrates Popular column. Provides good balance of high efficiency and ruggedness
COREGEL WA-1 Wine Analysis 8 H 8 Rugged design allows for little sample prep. High resolution makes the WA1 excellent for QA Higher efficiency but as robust as the work horse 87H3 column
COREGEL ION-310 8 H 11 Designed for fast analysis of organic acids and alcohols Ideal for the analysis of borate and bicarbonate
COREGEL ARH-601 6 H 8 Designed for the separation of aromatic organic acids Uses aqueous mobile phases
COREGEL-64H 6 H 10 Versatile column for organic acids, alcohols and carbohydrates Provides good balance of high efficiency and ruggedness
COREGEL USP L-17 8 H 8 Complies with USP L-17 specifications for the separation of citric, lactic, and acetic acid; can also separate a wide number of other organic acids Hydrogen form ion-exclusion column
DESCRIPTION SIZE COREGEL-87H1 COREGEL-87H3 COREGEL ION-300 COREGEL-107H COREGEL ORH-801
Column 7.8 x 300 mm – ICE-99-9861 ICE-99-9850 ICE-99-9866 –
Column (Fast) 7.8 x 200 mm – – – – ICE-99-9753
Column 7.8 x 150 mm – ICE-99-9865 – – –
Column 7.8 x 100 mm ICE-99-5861 – – – –
Column 6.5 x 300 mm – ICE-99-9871 – – ICE-99-9754
Column (Fast) 6.5 x 150 mm – – – ICE-99-9867 –
Guard Kit (1 holder, 2/pk cartridges)   ICE-99-2355 ICE-99-2361 ICE-99-2358 ICE-99-2356 ICE-99-2354
Guard Cartridges (2/pk)   – ICE-99-2371 ICE-99-2368 ICE-99-2366 ICE-99-2364
DESCRIPTION SIZE COREGEL WA-1 Wine COREGEL ION-310 COREGEL ARH-601 COREGEL-64H COREGEL USP L-17
Column 7.8 x 300 mm ICE-99-9810 – – ICE-99-9860 –
Column (Fast) 6.5 x 100 mm – ICE-99-7752 ICE-99-5753 – –
Column 4.1 x 250 mm – – – – ICE-99-8461
Guard Kit (1 holder, 2/pk cartridges)   ICE-99-3510 ICE-99-2357 ICE-99-2353 ICE-99-2360 ICE-99-2352
Guard Cartridges (2/pk)   ICE-99-1310 ICE-99-2367 ICE-99-2363 ICE-99-2370 ICE-99-2362
Key Definitions â–¸
Eluent Strength
The acidic concentration of the mobile phase, which directly influences organic acid retention. Stronger acidic eluents increase analyte interaction with the stationary phase, extending retention times and improving separation selectivity.
pH Stability Range (0–14)
The full spectrum of acidic to basic conditions the column can withstand without degradation, allowing aggressive cleaning, flexible method development, and reliable retention across diverse acid chemistries.
Isocratic Separation
A chromatographic method performed with a constant mobile phase composition. Concise Separations organic acid columns achieve strong separation power using only dilute acid, eliminating gradient complexity.
pKa–Retention Relationship
The influence of an analyte’s acid dissociation constant (pKa) on its chromatographic behavior. Adjusting eluent strength shifts the ionization state and retention of organic acids, enabling fine-tuned selectivity.
Self-Regenerating Eluent
A simple dilute acid mobile phase that continuously cleans the stationary phase during operation, reducing fouling, extending column lifetime, and eliminating the need for high-cost organic solvents.
Frequently Asked Questions â–¸

Why are Concise Separations columns ideal for organic acid analysis?

These columns offer full pH stability (0–14), high thermal tolerance, and strong retention control using simple dilute acid eluents—delivering reliable separations without gradients or expensive solvents.

Do I need organic solvents for these methods?

No. The mobile phase is typically a simple dilute acid, which eliminates the need for expensive organic solvents and reduces waste disposal costs.

How does eluent strength affect retention times?

Increasing the acidity (strength) of the eluent increases analyte interaction with the stationary phase, resulting in longer retention times and improved separation of closely related organic acids.