InfinityLab Poroshell 120

Agilent Poroshell 120 Reversed Phase HPLC Columns

Agilent InfinityLab Poroshell 120 columns deliver high efficiency, fast separations, and reduced backpressure—making them the trusted choice for both conventional LC and UHPLC systems. Their unique solid-core design and 2 µm frit construction allow reliable performance even with challenging or dirty samples.

Up to 50% lower backpressure than sub-2 µm particles—ideal for 400/600 bar LC and UHPLC systems.
High-speed performance from a solid core with a porous outer layer that enhances mass transfer and resolution.
Tight particle size distribution for excellent precision, reproducibility, and peak shape.

Multiple Particle Sizes for Any Method

The Poroshell 120 family is available in 1.9 µm, 2.7 µm, and 4 µm formats—supporting everything from high-speed UHPLC analysis to robust routine HPLC workflows. Whether separating small polar analytes or complex mixtures, Poroshell chemistries provide the selectivity and speed required for modern labs.

Convenient Kits for Method Validation

For quality-control workflows, Agilent offers method validation kits containing columns from three different manufacturing lots. Add a “K” to any Poroshell part number to streamline lot-to-lot reproducibility testing.

Poroshell 120 HILIC Options for Polar Compounds

The newest Poroshell 120 HILIC phases are engineered for fast, high-efficiency separation of polar and highly charged compounds using standard LC systems.

HILIC-Z — Zwitterionic phase offering excellent peak shape for highly charged compounds under high-pH or elevated-temperature conditions.
HILIC-OH5 — Poly-hydroxy fructan phase providing orthogonal selectivity for difficult polar analytes.

Why Choose Agilent Poroshell 120?

High efficiency and resolution across all LC platforms.
Rugged performance with dirty or complex samples.
Long column lifetime and consistent batch-to-batch reproducibility.
Versatile chemistries supporting reversed-phase, HILIC, and polar workflows.

For help selecting the right Poroshell 120 column, contact our technical support team .

Key Definitions ▸

Superficially Porous Particles (SPP)

Engineered with a solid core and a porous outer layer, SPP particles reduce diffusion distance, improving efficiency and resolution while lowering backpressure—core to Poroshell 120 performance.

2 µm Frit Technology

A specialized frit that resists clogging from dirty samples, improving uptime and extending column lifetime compared to traditional sub-2 µm UHPLC columns.

Hydrophilic Interaction Chromatography (HILIC)

A separation mode optimized for polar analytes, using high organic mobile phases. Poroshell 120 HILIC-Z and HILIC-OH5 provide selectivity for charged and highly polar compounds.

Method Validation Kit

An Agilent kit providing three column lots of the same phase to verify robustness, consistency, and batch-to-batch reproducibility during regulated method validation.

Narrow Particle Size Distribution

Highly uniform particle sizing improves efficiency, resolution, and reproducibility—critical for consistent Poroshell 120 chromatographic performance.

Frequently Asked Questions ▸

What makes Poroshell 120 columns different from traditional sub-2 µm columns?

Poroshell 120 columns use solid-core particle technology, delivering UHPLC-level efficiency with up to 50% lower backpressure—making them ideal for both UHPLC and conventional 400/600 bar LC systems.

Can Poroshell 120 columns handle dirty or complex samples?

Yes. Their 2 µm frits and core–shell design better tolerate particulates and sample matrices, helping reduce fouling while maintaining high efficiency and superior peak shape.

What are Poroshell 120 HILIC columns used for?

Poroshell 120 HILIC-Z and HILIC-OH5 phases are designed for high-efficiency separation of small polar analytes, offering strong retention, excellent peak shape, and alternate selectivity on standard LC systems.

What are method validation kits, and when should I use them?

Agilent’s three-lot validation kits ensure reproducibility and regulatory confidence by including columns from different manufacturing lots—ideal for QC labs performing method transfer or validation.

Product List

SKU	Name	LC Column Phase	Particle Size	Inner Diameter (ID)	Length
SKU 699768-901T	Name 699768-901T - Poroshell 120 Bonus-RP, 2.1X50 mm, 2.7 um, with Column ID	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 2.1 mm	Length 50 mm
SKU 695968-901T	Name 695968-901T - Poroshell 120 Bonus-RP, 4.6X100 mm, 2.7 um, with Column ID	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 4.6 mm	Length 100 mm
SKU 695968-301T	Name 695968-301T - Poroshell 120 Bonus-RP, 3X100 mm, 2.7 um, with Column ID	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 3 mm	Length 100 mm
SKU 693968-901T	Name 693968-901T - Poroshell 120 Bonus-RP, 4.6X150 mm, 2.7 um, with Column ID	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 4.6 mm	Length 150 mm
SKU 693968-301T	Name 693968-301T - Poroshell 120 Bonus-RP, 3X150 mm, 2.7 um, with Column ID	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 3 mm	Length 150 mm
SKU 695768-901T	Name 695768-901T - Poroshell 120 Bonus-RP, 2.1X100 mm, 2.7 um, with Column ID	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 2.1 mm	Length 100 mm
SKU 693768-901T	Name 693768-901T - Poroshell 120 Bonus-RP, 2.1X150 mm, 2.7 um, with Column ID	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 2.1 mm	Length 150 mm
SKU 699968-901T	Name 699968-901T - Poroshell 120 Bonus-RP, 4.6X50 mm, 2.7 um, with Column ID	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 4.6 mm	Length 50 mm
SKU 699968-301T	Name 699968-301T - Poroshell 120 Bonus-RP, 3X50 mm, 2.7 um, with Column ID	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 3 mm	Length 50 mm
SKU 699968-901K	Name 699968-901K - Poroshell 120 BonusRP,4.6mm,50mm,2.7um LC Col	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 4.6 mm	Length 50 mm
SKU 699968-901	Name 699968-901 - Poroshell 120 Bonus RP,4.6x50mm,2.7um	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 4.6 mm	Length 50 mm
SKU 699968-301K	Name 699968-301K - Poroshell 120 BonusRP,3.0mm,50mm,2.7um LC Col	LC Column Phase Bonus-RP	Particle Size 2.7 um	Inner Diameter (ID) 3 mm	Length 50 mm

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