HP-5ms Low-Bleed

HP-5ms Low-Bleed

HP-5ms Capillary Columns

Agilent J&W HP-5ms capillary columns feature a (5%-phenyl)-methylpolysiloxane stationary phase engineered for consistently low bleed and high thermal stability—making them a preferred choice for GC/MS applications that demand reliable sensitivity and clean mass spectral baselines. The phase chemistry provides predictable non-polar selectivity and broad applicability across volatile, semi-volatile, and mid-boiling analytes.

Each HP-5ms column is bonded, cross-linked, and fully solvent-rinsable, ensuring long-term durability even under rigorous analytical conditions. This construction improves inertness and minimizes adsorption or degradation of active compounds—including acidic, basic, or polar analytes—delivering sharp peak shapes and enhanced reproducibility. The extremely low bleed profile enhances signal-to-noise ratios and supports superior mass spectral integrity for trace-level quantitation.

HP-5ms columns are also considered equivalent to USP Phase G27, making them suitable for regulated workflows, validated methods, and cross-laboratory method alignment. Their robust performance and broad analyte coverage make them an industry-standard choice for environmental testing, forensic toxicology, petrochemical profiling, food and fragrance analysis, and general GC/MS applications where sensitivity, stability, and column longevity are essential.

Key Definitions â–¸
(5%-Phenyl)-Methylpolysiloxane
A widely used non-polar GC stationary phase that blends methylpolysiloxane with 5% phenyl functional groups. This composition provides predictable retention patterns and broad compatibility for volatile and semi-volatile analytes across GC and GC/MS workflows.
Low-Bleed Column
A capillary column engineered to minimize stationary-phase volatilization at elevated temperatures. Low bleed leads to cleaner baselines, higher signal-to-noise ratios, and improved mass spectral accuracy—making it essential for GC/MS sensitivity.
Bonded and Cross-Linked
A durable column construction where the stationary phase is chemically bonded to the inner wall and cross-linked for added strength. This allows solvent rinsing, high-temperature operation, and extended column lifetime—especially important in demanding GC/MS methods.
USP Phase G27
A United States Pharmacopeia stationary-phase classification that corresponds to (5%-phenyl)-methylpolysiloxane selectivity. HP-5ms columns meet this equivalency, enabling use in validated and regulatory analytical methods.
Mass Spectral Integrity
The degree to which mass spectra remain clean, accurate, and free from column-derived interference. Low-bleed HP-5ms columns ensure minimal background noise, preserving spectral purity and supporting confident compound identification in GC/MS.
Frequently Asked Questions â–¸
What makes HP-5ms columns suitable for GC/MS applications?
HP-5ms columns exhibit very low bleed at elevated temperatures, producing cleaner baselines and more reliable mass spectral data. Their (5%-phenyl)-methylpolysiloxane phase delivers predictable non-polar selectivity and excellent inertness, making them ideal for GC/MS workflows across many industries.
How does HP-5ms compare to USP Phase G27?
HP-5ms is considered equivalent to USP Phase G27, aligning with the same 5% phenyl methylpolysiloxane selectivity. This makes it suitable for validated or regulated methods that require this phase class while offering enhanced low-bleed characteristics optimal for GC/MS.
What types of compounds benefit most from HP-5ms inertness?
HP-5ms columns provide excellent inertness for active and adsorptive analytes such as pesticides, amines, phenols, and other acidic or basic compounds. This prevents adsorption, improves peak shape, and enhances quantitative accuracy at both trace and mid-level concentrations.
Can HP-5ms columns be solvent-rinsed to extend usability?
Yes. Because HP-5ms columns are bonded and cross-linked, they are compatible with solvent rinsing. This allows analysts to remove non-volatile contaminants or matrix residue, restoring performance and extending column lifetime in demanding workflows.
What industries commonly use HP-5ms columns?
HP-5ms columns are widely used in environmental testing labs, forensic toxicology, petrochemical characterization, pharmaceutical impurity analysis, food and fragrance profiling, and general GC/MS testing that requires high sensitivity and stable chromatographic performance.