Filter Plates

High Throughput 96-Well Filter Plates

Chrom Tech supplies high-performance 96-well filter plates designed for rapid, parallel sample preparation in analytical and life science workflows. These plates support customizable membrane chemistries and pore sizes, allowing laboratories to optimize flow rates, retention, and recovery based on their specific application requirements.

Engineered for sensitive techniques such as LC-MS and semi-automated high-throughput assays, Chrom Tech filter plates utilize low-extractable, low-binding membranes to ensure clean sample preparation and reliable quantification. Broad chemical compatibility enables use across aqueous, organic, and mixed solvent systems.

Key Features

  • Selectable filter membrane materials for application-specific performance
  • Multiple pore size options for optimized flow and particulate retention
  • Available in several plate formats
  • Automation-friendly design for seamless robotic integration
  • Supports sample volumes up to 1 mL per well
  • Standard SBS footprint for compatibility with automated workstations and microplate systems
  • Skirted edges provide stable robotic handling equivalent to conventional microtitration plates

Molecular Weight Cut-Off (MWCO) 96-Well Filter Plates

MWCO filter plates are designed for molecular separation and concentration workflows where precise size-based filtration is required. Common applications include:

  • Separating large molecules from small molecules
  • Removing unincorporated labels after protein modification reactions
  • Concentrating protein peaks following gel-permeation chromatography
  • Protein enrichment from tissue culture media, antisera, or antibody solutions
  • Desalting and buffer exchange after ion-exchange or affinity chromatography

For laboratories not currently using 96-well plate formats, Chrom Tech also offers alternative filtration solutions including syringe filters and filter vials.

Chrom Tech filter plates and tubes are manufactured from DNase/RNase- and protease-free certified plastics. Products are non-sterile by default, but may be autoclaved when required. Recommended autoclave conditions are 121°C (250°F) at 15 psi for 15 minutes.

PART NO
MEMBRANE
PORE SIZE
 VOL/WELL
96 WELL FILTER PLATES
96F-NY020 Nylon 0.2 µm 1 mL
★ CT-9645 Nylon 0.45 µm 300 µL
96F-NY045 Nylon 0.45 µm 1 mL
96F-PT020 PTFE (polytetrafluoroethylene) 0.2 µm 1 mL
★ OC-9279020 PTFE (polytetrafluoroethylene) 0.2 µm 2 mL
96F-PT045 PTFE (polytetrafluoroethylene) 0.45 µm 1 mL
96F-PV020 PVDF (polyvinylidene difluoride) 0.2 µm 1 mL
96F-PV045 PVDF (polyvinylidene difluoride) 0.45 µm 1 mL
96F-PS020 PES (polyethersulfone) 0.2 µm 1 mL
96F-PS045 PES (polyethersulfone) 0.45 µm 1 mL
96F-PS045-2 PES (polyethersulfone) 0.45 µm 2 mL
96F-CA020 CA (cellulose acetate) 0.2 µm 1 mL
96F-CA045 CA (cellulose acetate) 0.45 µm 1 mL
96F-PP020 PP (polypropylene) 0.2 µm 1 mL
96F-PP045 PP (polypropylene) 0.45 µm 1 mL
96F-PP045-2 PP (polypropylene) 0.45 µm 2 mL
A5960002 Captiva PP (polypropylene) 0.2 µm 1 mL
A5960045 Captiva PP (polypropylene) 0.45 µm 1 mL
A596002000 Captiva PP (polypropylene) 20 µm 1 mL
96F-RC020 RC (regenerated cellulose) 0.2 µm 1 mL
96F-RC045 RC (regenerated cellulose) 0.45 µm 1 mL
96F-NC020 NC (nitrocellulose) 0.2 µm 1 mL
★ TOP SELLER!
PART NO MEMBRANE MWCO VOL/WELL
MOLECULAR WEIGHT CUT-OFF 96 WELL FILTER PLATES
201013-100 PES (polyethersulfone) 10K 0.4 mL
201015-100 PES (polyethersulfone) 30K 0.4 mL
201596-100 PES (polyethersulfone) 100K 0.4 mL
201598-100 PES (polyethersulfone) 300K 0.4 mL
Membrane Selection Guide ▶
Membrane Aqueous Organic Low Protein
Binding 		Application
Nylon ● ●   General laboratory and HPLC sample filtration prior to injection. Solvent resistant with low extractables; not ideal when protein recovery is critical.
PTFE   ●   Hydrophobic and chemically resistant to most solvents, acids, and bases. Low extractables; can block water vapor (useful for transducer protection).
PVDF ● ● ● Solvent resistant membrane with low UV-adsorbing extractables. Used for HPLC sample filtration and general biological filtration; low protein binding.
PES ● ● ● Mechanically strong with low inorganic extractables. Typical uses include ion chromatography, tissue culture filtration, and filtration of proteins and nucleic acids.
Cellulose Acetate ●   ● Very low protein binding (often less than PVDF) and ideal for aqueous samples. Excellent when maximum protein recovery in filtrate is critical.
PP ● ●   Hydrophilic membrane with broad chemical compatibility to many organic solvents. A strong choice for HPLC sample filtration in protein analysis workflows.
RC ● ● ● Regenerated Cellulose is hydrophilic, solvent resistant, and low protein binding. Ideal for removing particulates from HPLC samples prior to injection; low water extractables.
NC ●     Nitrocellulose (mixed esters) filters are hydrophilic and made from cellulose nitrate/cellulose acetate blends. Fast flow rates due to a uniform membrane structure.
Legend: ● = compatible / recommended.
Key Definitions ▶
96-Well Filter Plate
A microplate-format filtration device that enables simultaneous processing of up to 96 samples for high-throughput clarification, cleanup, or concentration workflows.
Membrane (Filter Membrane)
The porous filtration material in each well that controls chemical compatibility, binding behavior, and particle or biomolecule retention during sample preparation.
Pore Size
The nominal opening size of the membrane (for example, 0.2 µm or 0.45 µm) that impacts flow rate and the size of particulates retained during filtration.
Vol/Well
The maximum recommended sample volume that each well is designed to process, helping ensure consistent filtration performance and reliable recovery.
SBS Footprint
Standardized microplate dimensions that ensure compatibility with automation platforms, microplate carriers, and common laboratory instruments.
Low-Binding / Low-Extractable
Membrane and plastic characteristics designed to minimize analyte adsorption and reduce leachables, supporting cleaner baselines and more consistent quantitative results.
Frequently Asked Questions ▶
What are 96-well filter plates used for?
96-well filter plates enable rapid, parallel filtration of multiple samples for workflows such as protein cleanup, desalting, concentration, and general sample clarification in high-throughput laboratories.
Can these plates be used with automated liquid handlers?
Yes. The standard SBS footprint and skirted plate design allow full compatibility with robotic systems, automated workstations, and common microplate accessories.
How do I choose between 0.2 µm and 0.45 µm pore sizes?
Use 0.2 µm membranes for finer particulate removal and cleaner samples, and 0.45 µm membranes when higher flow rates are desired and larger particles are acceptable.
Are the filter plates sterile?
Plates are supplied non-sterile but are manufactured from DNase/RNase- and protease-free certified plastics. They may be autoclaved when sterile conditions are required.
What applications benefit most from MWCO filter plates?
MWCO plates are commonly used for protein concentration, buffer exchange, removal of unincorporated labels, and molecular separation based on size exclusion.