ASI Static Mixers

ASI Mixers
  • Interchangeable mixer cartridges for flexible volume selection
  • Optimized mixing efficiency to improve gradient performance
  • Available in in-line, binary, and ternary configurations
  • Offered in stainless steel or PEEK constructions
  • Enhanced reaction efficiency for post-column derivatization

ASI Static Mixers are engineered to address the most demanding high-pressure mixing challenges encountered in modern chromatography. Featuring advanced internal geometries, ASI mixers deliver consistent, reproducible solvent blending across a wide range of flow rates, making them ideal for UHPLC, analytical HPLC, and reaction-based workflows.

ASI’s proprietary HyperShear™ mixing technology employs a highly efficient cross-flow shearing mechanism that generates vortex-based mixing rather than relying on conventional packed beds or tortuous flow paths. This design typically provides 25% to 200% greater mixing efficiency compared to traditional static mixers, resulting in smoother gradients and improved chromatographic fidelity.

Within each mixer flow series, interchangeable mixer cartridges allow users to fine-tune mixing volume without replacing the entire assembly. This modular approach simplifies optimization while reducing cost and downtime. In contrast, UHPLC mixers are factory-tested and pressure-rated as complete assemblies, ensuring reliable performance at pressures up to 18,000 PSI.

Selecting the appropriate static mixer involves balancing delay volume, mixing noise, gradient accuracy, and overall chromatographic performance. ASI Static Mixers provide the flexibility and performance needed to achieve optimal results across a broad range of applications.

INLINE BINARY TEE TERNARY TEE MIXER CARTRIDGE
VOLUME (µL)		 RECOMMENDED FLOW RATES
(µL/min)		 REPLACEMENT CARTRIDGE
SS MICRO FLOW SERIES – COMPLETE ASSEMBLIES (CARTRIDGE & HOUSINGS)
401-0005 402-0005 403-0005 50 – 5 400-0005
401-0010 402-0010 403-0010 105 – 10 400-0010
401-0025 402-0025 403-0025 2510 – 20 400-0025
PEEK MICRO FLOW SERIES – COMPLETE ASSEMBLIES (CARTRIDGE & HOUSINGS)
401-0005B 402-0005B 403-0005B 50 – 5 400-0005B
401-0010B 402-0010B 403-0010B 105 – 10 400-0010B
401-0025B 402-0025B 403-0025B 2510 – 20 400-0025B
SS LOW FLOW SERIES – COMPLETE ASSEMBLIES (CARTRIDGE & HOUSINGS)
411-0050 412-0050 413-0050 5020 – 150 410-0050
— ★ 412-0100 — 100100 – 250 410-0100
411-0150 412-0150 413-0150 150150 – 500 410-0150
411-0250 412-0250 413-0250 250500+ 410-0250
PEEK LOW FLOW SERIES – COMPLETE ASSEMBLIES (CARTRIDGE & HOUSINGS)
411-0050B 412-0050B 413-0050B 5020 – 150 410-0050B
— ★ 412-0100B — 100100 – 250 410-0100B
411-0150B 412-0150B 413-0150B 150150 – 500 410-0150B
411-0250B 412-0250B 413-0250B 250500+ 410-0250B
SS UHPLC SERIES – COMPLETE ASSEMBLIES (SOLD AS COMPLETE ASSEMBLY ONLY)
401-0005HP 402-0005HP 403-0005HP 50 – 5 —
401-0010HP 402-0010HP 403-0010HP 105 – 10 —
401-0025HP 402-0025HP 403-0025HP 2510 – 20 —
411-0050HP 412-0050HP 413-0050HP 5020 – 150 —
411-0150HP 412-0150HP 413-0150HP 150150 – 500 —
411-0250HP 412-0250HP 413-0250HP 250500+ —

★ Top Seller  |  Other configurations are available. Please contact Chrom Tech for more information.

TECH TIP
Choosing the right size mixer is a trade off between delay volume, mixing noise, gradient fidelity and chromatographic performance.
  • ➜ For any given flow rate, the more the mixing volume the better the mixing, and the lower the baseline noise.
  • ➜ The smaller the mixing volume, the better the definition and sharpness of linear gradients.
  • ➜ Multi-pump high pressure gradient systems typically require far less mixing volume than low pressure single pump systems when running linear gradients.
  • ➜ An ASI 150 µL in-line static mixer can be added in addition to the standard onboard mixer to further reduce mixing noise.
Key Definitions ▸
Static Mixer
A passive mixing device that blends fluid streams using internal flow geometry rather than moving parts, commonly used in HPLC and UHPLC systems to improve gradient homogeneity.
Mixing Volume
The internal volume of a mixer that directly influences gradient smoothing, baseline noise, and delay volume within a chromatographic system.
HyperShear™ Mixing Technology
ASI’s proprietary cross-flow shearing mechanism that generates controlled vortex mixing, delivering significantly higher mixing efficiency than traditional packed-bed or tortuous-path mixers.
Mixer Cartridge
A replaceable internal component that defines the effective mixing volume of an ASI static mixer, allowing users to optimize performance without replacing the entire mixer housing.
Delay Volume
The additional system volume introduced by a mixer that can affect gradient response time and retention behavior, especially in fast or low-volume LC methods.
Gradient Fidelity
A measure of how accurately a programmed solvent gradient is delivered at the column inlet, influenced by mixer efficiency, flow rate, and system configuration.
Binary and Ternary Mixing
Solvent blending configurations where two (binary) or three (ternary) mobile phases are combined, each requiring appropriate mixer design and volume to achieve stable, reproducible gradients.
Frequently Asked Questions ▸
What does a static mixer do in an HPLC or UHPLC system?
A static mixer blends two or more solvent streams into a uniform mobile phase before the column, helping stabilize gradients, reduce baseline ripple, and improve run-to-run reproducibility.
How do I choose the right ASI mixer volume?
Mixer selection is a tradeoff between mixing quality and delay volume. Larger mixing volumes typically improve mixing and reduce baseline noise, while smaller volumes preserve gradient sharpness and minimize delay—especially in fast LC methods.
Why do multi-pump high-pressure gradient systems often need less mixing volume?
High-pressure gradient systems commonly provide better upstream solvent proportioning and can require less added mixing volume to achieve stable gradients, compared to low-pressure proportioning systems running linear gradients.
What is the advantage of ASI’s interchangeable mixer cartridges?
Within a given ASI mixer series, cartridges can be swapped to change mixing volume without replacing the full assembly, making method optimization faster and more economical.
When should I consider adding an in-line static mixer to my system?
Consider adding an in-line mixer when you see mixing-related baseline noise, poor gradient reproducibility, or inconsistent retention—especially with challenging solvent combinations or demanding gradient profiles.
Are ASI static mixers used for post-column derivatization?
Yes. Static mixers can improve reaction efficiency by quickly and consistently blending the derivatization reagent with the column effluent in high-pressure post-column workflows.