Agilent J&W GC/MS Columns

Agilent J&W GC/MS Columns

Agilent J&W GC/MS columns are engineered for ultra-low bleed, high thermal stability, and consistent inertness—supporting high-sensitivity mass spectrometry workflows across environmental, pharmaceutical, food safety, petrochemical, and forensic laboratories.

The portfolio includes industry-standard phases such as DB-1ms, DB-5ms, HP-5ms, DB-35ms, DB-17ms, DB-624 Ultra Inert, DB-WAX Ultra Inert, DB-225ms, DB-XLB, and DB-UI 8270, each optimized for specific GC/MS selectivity profiles and regulatory methods. Low-bleed stationary phases minimize background signal and column bleed, improving spectral clarity and signal-to-noise ratios—especially at elevated temperatures.

Low-Bleed Performance for GC/MS

Agilent “ms” designated columns are specifically formulated to reduce stationary-phase bleed, preserving detector sensitivity and protecting ion sources. This makes them ideal for semi-volatile organic compounds (SVOCs), VOCs, pesticide panels, residual solvents, EPA 8270/624 workflows, and broad GC/MS screening methods.

Ultra Inert Technology

Ultra Inert (UI) versions provide enhanced surface deactivation to minimize adsorption of active compounds such as acids, bases, phenols, and polar analytes. The result is sharper peaks, improved recovery at trace levels, and greater reproducibility in regulated laboratories.

Selectivity Options Across Polarity Range

From non-polar 100% dimethylpolysiloxane phases (DB/HP-1ms) to 5% phenyl workhorse columns (DB/HP-5ms), mid-polar phenyl phases (DB-17ms, DB-35ms), cyanopropyl-containing selectivity (DB-225ms), polar PEG (DB-WAX UI), and method-specific formats like DB-624 UI and DB-UI 8270, Agilent J&W GC/MS columns provide controlled retention and orthogonal selectivity for even the most challenging mixtures.

Agilent J&W GC/MS Columns — Phase Descriptions ▸
DB-WAX Ultra Inert
Polar PEG phase • Ultra Inert deactivation • oxygenates & alcohols
▸

DB-WAX Ultra Inert is a highly polar polyethylene glycol (PEG) phase optimized for oxygenated compounds, alcohols, aldehydes, and free acids in GC/MS workflows where active analytes require superior inertness.

When to Choose This Phase
  • Flavor & fragrance analysis
  • Alcohols and oxygenated solvents
  • Acid-sensitive compounds requiring improved recovery
Key Characteristics
PolarityPolar (PEG)
Surface TreatmentUltra Inert deactivation
Best ForOxygenates, acids, flavors
DB-1ms Low-Bleed
Nonpolar dimethyl • Low bleed • GC/MS optimized
▸

DB-1ms Low-Bleed is a 100% dimethylpolysiloxane nonpolar column engineered for minimal bleed in mass spectrometry applications.

When to Choose
  • Hydrocarbon profiling
  • Volatility-driven separations
  • General GC/MS screening
Key Characteristics
PolarityNonpolar
Bleed ProfileLow bleed (MS optimized)
Best ForHydrocarbons, VOC screening
DB-1ms Ultra Inert
Nonpolar • Enhanced deactivation • Active analytes
▸

DB-1ms Ultra Inert provides nonpolar selectivity with advanced surface deactivation for improved peak symmetry and trace-level sensitivity.

Applications
  • SVOC screening
  • Trace-level environmental analysis
  • Active compound recovery
PolarityNonpolar
SurfaceUltra Inert
Best ForTrace analytes
HP-1ms Low-Bleed
Nonpolar • HP-series GC/MS workhorse
▸

HP-1ms Low-Bleed delivers reliable nonpolar GC/MS separations with minimal background signal.

  • Boiling point-driven separations
  • General MS screening
  • Routine VOC/SVOC methods
PolarityNonpolar
Bleed ProfileLow bleed
ApplicationRoutine GC/MS
HP-1ms Ultra Inert
Nonpolar • Enhanced inertness • Trace analysis
▸

Enhanced inert HP-1ms version improving peak symmetry and reducing adsorption for active analytes.

PolarityNonpolar
SurfaceUltra Inert
Best ForTrace-level detection
DB-5ms Low-Bleed
5% phenyl • Industry standard • GC/MS
▸

Balanced low-polarity selectivity widely used for environmental, pesticide, and semivolatile analysis.

PolarityLow polarity (5% phenyl)
Bleed ProfileLow bleed
Best ForGeneral GC/MS workflows
DB-5ms Ultra Inert
Enhanced deactivation • Active analytes
▸

Ultra Inert version of DB-5ms for improved active compound recovery and peak shape.

PolarityLow polarity (5% phenyl)
SurfaceUltra Inert
Best ForPesticides & trace SVOCs
HP-5ms Low-Bleed
Versatile 5% phenyl • Routine GC/MS
▸

HP-series 5% phenyl phase optimized for wide analyte range and robust MS performance.

PolarityLow polarity
BleedLow
ApplicationGeneral MS screening
HP-5ms Ultra Inert
Enhanced inert 5% phenyl
▸

Improved deactivation for sensitive analytes and trace-level GC/MS detection.

PolarityLow polarity
SurfaceUltra Inert
ApplicationTrace pesticide & SVOC
DB-XLB Low-Bleed
Optimized selectivity • Pesticides & aromatics
▸

Low-bleed MS column optimized for improved aromatic separation and pesticide workflows.

PolarityLow polarity (optimized)
BleedLow
Best ForPesticides, aromatics
DB-35ms Low-Bleed
35% phenyl • Mid-polar GC/MS
▸

Mid-polar 35% phenyl phase improving aromatic resolution and PAH separations.

PolarityMid polarity
BleedLow
Best ForPAHs, complex mixtures
DB-35ms Ultra Inert
Enhanced mid-polar inert surface
▸

Enhanced inertness for active analytes in demanding GC/MS methods.

PolarityMid polarity
SurfaceUltra Inert
ApplicationRegulatory workflows
DB-17ms Low-Bleed
50% phenyl • Aromatic/isomer selectivity
▸

Mid-high polarity phase enhancing aromatic resolution and structural isomer separations.

PolarityMid-high polarity
BleedLow
Best ForIsomers, aromatic compounds
DB-225ms
Cyanopropyl phenyl • Polar selectivity
▸

Polar phase providing unique selectivity for halogenated and pesticide compounds.

PolarityPolar
Best ForPesticides, halogenated analytes
DB-624 Ultra Inert
VOC & residual solvent optimized
▸

Optimized for VOC and residual solvent analysis with ultra inert surface chemistry.

PolaritySpecialty (VOC focused)
SurfaceUltra Inert
Best ForResidual solvent, EPA methods
DB-UI 8270D
EPA 8270 SVOC optimized
▸

Method-specific column engineered for EPA 8270 SVOC workflows with optimized recoveries.

ApplicationEPA 8270 SVOC
FocusImproved recoveries
RegulatoryMethod compliant
DB-WAX Ultra Inert

Polar PEG phase for oxygenates, alcohols, and free acids requiring superior inertness.

Highlights
  • PEG selectivity
  • Improved active compound recovery
  • Flavor & solvent analysis
DB-1ms Low-Bleed

100% dimethylpolysiloxane phase for non-polar GC/MS separations.

  • Ultra-low MS bleed
  • High thermal stability
  • Hydrocarbon profiling
DB-1ms Ultra Inert

Non-polar phase with enhanced deactivation for active analytes.

  • Improved peak symmetry
  • Trace-level sensitivity
  • SVOC screening
HP-1ms Low-Bleed

Agilent HP-series non-polar workhorse for GC/MS.

  • Low bleed profile
  • Boiling point separations
  • General MS screening
HP-1ms Ultra Inert

Enhanced inert version of HP-1ms for improved analyte recovery.

  • Reduced adsorption
  • Better peak shape
  • Trace-level applications
DB-5ms Low-Bleed

5% phenyl phase delivering balanced selectivity for complex mixtures.

  • Industry standard
  • Excellent MS compatibility
  • Environmental methods
DB-5ms Ultra Inert

Enhanced inert version of DB-5ms for active compounds.

  • Improved peak symmetry
  • Reduced active sites
  • Pesticide analysis
HP-5ms Low-Bleed

Versatile 5% phenyl phase for routine GC/MS workflows.

  • Low bleed
  • Wide analyte range
  • Robust performance
HP-5ms Ultra Inert

Improved deactivation for sensitive analytes and trace analysis.

  • Enhanced inertness
  • Sharper peak shape
  • Trace-level detection
DB-XLB Low-Bleed

Low bleed phase with optimized selectivity for pesticides and aromatics.

  • Reduced phenyl content
  • Improved isomer separation
  • GC/MS compatible
DB-35ms Low-Bleed

35% phenyl phase enhancing aromatic resolution.

  • Mid-polar selectivity
  • Improved PAH separation
  • Low bleed MS format
DB-35ms Ultra Inert

Enhanced inert mid-polar column for demanding GC/MS methods.

  • Better active compound recovery
  • Improved peak symmetry
  • Regulatory workflows
DB-17ms Low-Bleed

50% phenyl phase for enhanced polarity and isomer separation.

  • Mid-high polarity
  • Aromatic resolution
  • Low bleed design
DB-225ms

Cyanopropyl phenyl phase for unique selectivity of halogenated compounds.

  • Polar selectivity
  • Pesticide applications
  • MS compatible
DB-624 Ultra Inert

Optimized for VOC and residual solvent analysis.

  • EPA 624 compliance
  • Ultra Inert surface
  • Volatile focus
DB-UI 8270D

Method-specific column engineered for EPA 8270 SVOC analysis.

  • SVOC optimized
  • Improved recoveries
  • Regulatory ready

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SKU Name ID Length Phase Film Thickness
SKU
122-4732
Name
122-4732 - DB-17MS 30m, 0.25mm, 0.25um 7in
ID
0.25 mm
Length
30 m
Phase
DB-17ms
Film Thickness
0.25 um
SKU
122-5532
Name
122-5532 - DB-5MS 30m, 0.25mm, 0.25um 7in ca
ID
0.25 mm
Length
30 m
Phase
DB-5ms
Film Thickness
0.25 um
SKU
122-5513
Name
122-5513 - DB-5MS 15m, 0.25mm, 1.00um 7in
ID
0.25 mm
Length
15 m
Phase
DB-5ms
Film Thickness
1 um
SKU
122-5512UI
Name
122-5512UI - DB-5MS Ultra Inert 15m, 0.25mm, 0.25um 7in
ID
0.25 mm
Length
15 m
Phase
DB-5ms
Film Thickness
0.25 um
SKU
121-5523UI
Name
121-5523UI - DB-5MS Ultra Inert 20m, 0.18mm, 0.36um 7in
ID
0.18 mm
Length
20 m
Phase
DB-5ms
Film Thickness
0.36 um
SKU
121-5522
Name
121-5522 - DB-5MS 20m, 0.18mm, 0.18um 7in ca
ID
0.18 mm
Length
20 m
Phase
DB-5ms
Film Thickness
0.18 um
SKU
121-5512UI
Name
121-5512UI - Agilent J&W DB-5ms Ultra Inert, 10 m, 0.18 mm
ID
0.18 mm
Length
10 m
Phase
DB-5ms
Film Thickness
0.18 um
SKU
123-7063UI-INT
Name
123-7063UI-INT - DB-WAX Ultra Inert Intuvo GC column module, 60 m, 0.32 mm
ID
0.32 mm
Length
60 m
Phase
DB-WAX
Film Thickness
0.5 um
SKU
123-7063UI
Name
123-7063UI - DB-WAX Ultra Inert, 60 m, 0.32 mm, 0.50 um
ID
0.32 mm
Length
60 m
Phase
DB-WAX
Film Thickness
0.5 um
SKU
122-5532-INT
Name
122-5532-INT - DB-5ms Intuvo GC column module, 30 m, 0.25 mm, 0.25 um
ID
0.25 mm
Length
30 m
Phase
DB-5ms
Film Thickness
0.25 um
SKU
123-7062UI
Name
123-7062UI - DB-WAX Ultra Inert, 60 m, 0.32 mm, 0.25 um
ID
0.32 mm
Length
60 m
Phase
DB-WAX
Film Thickness
0.25 um
SKU
122-5512UI-INT
Name
122-5512UI-INT - DB-5ms Ultra Inert Intuvo GC column module, 15 m, 0.25 mm
ID
0.25 mm
Length
15 m
Phase
DB-5ms
Film Thickness
0.25 um
Key Definitions ▸
Low-Bleed (ms) Columns
GC columns specifically formulated to minimize stationary-phase bleed at high temperatures. Reduced bleed improves MS sensitivity, spectral clarity, and detector stability, making them ideal for GC/MS applications.
Ultra Inert (UI) Deactivation
Enhanced surface deactivation technology that reduces adsorption of active compounds such as acids, bases, phenols, and polar analytes. Ultra Inert columns deliver sharper peaks, improved recovery, and better reproducibility in trace-level analysis.
Phenyl Content (%)
The percentage of phenyl groups incorporated into the polysiloxane stationary phase. Increasing phenyl content (e.g., 5%, 35%) changes selectivity and improves separation of aromatic and halogenated compounds.
Non-Polar vs. Mid-Polar Phases
Non-polar phases (e.g., DB-1ms) separate primarily by boiling point, while mid-polar phases (e.g., DB-5ms, DB-35ms) introduce aromatic interactions to improve resolution of structurally similar compounds.
Method-Specific GC Columns (624 / 8270)
Columns engineered to meet regulatory method requirements such as EPA 624 (VOCs) and EPA 8270 (SVOCs). These formats balance selectivity, bleed performance, and inertness for compliance-driven environmental analysis.
Frequently Asked Questions ▸
What does “ms” mean in DB-5ms or DB-1ms?
“ms” indicates the column is optimized for mass spectrometry with low stationary-phase bleed. Low bleed improves baseline stability, reduces background noise, and enhances signal-to-noise performance in GC/MS analysis.
When should I choose an Ultra Inert (UI) column?
Choose Ultra Inert columns when analyzing active compounds such as acids, bases, phenols, pesticides, or other adsorption-prone analytes. UI technology minimizes surface activity, improving peak shape, reproducibility, and trace-level recovery.
How do I choose between DB-1ms and DB-5ms?
DB-1ms (100% dimethylpolysiloxane) is fully non-polar and separates primarily by boiling point. DB-5ms (5% phenyl) introduces additional aromatic selectivity, making it the more versatile “workhorse” column for semi-volatiles and complex mixtures.
What is the benefit of higher phenyl content (e.g., DB-35ms)?
Increasing phenyl content enhances interaction with aromatic compounds, improving resolution of structurally similar analytes such as pesticides, PAHs, and halogenated compounds. DB-35ms is often used when DB-5ms cannot fully resolve critical pairs.
Are DB-624 UI and DB-UI 8270 method-specific columns?
Yes. DB-624 Ultra Inert is commonly used for VOC and residual solvent analysis, while DB-UI 8270 is engineered for EPA 8270 semi-volatile methods. Both are optimized for regulatory compliance, reproducibility, and inertness.