Agilent HPLC Columns

ZORBAX, Poroshell, Polaris & Pursuit HPLC Columns

Chrom Tech supplies a complete portfolio of Agilent HPLC columns for small-molecule analysis, including superficially porous InfinityLab Poroshell 120 columns and fully porous ZORBAX, Polaris, and Pursuit series. Together, these families support an exceptionally wide range of separation modes, selectivity options, and analytical workflows.

Reversed-phase LC remains the dominant technique for small-molecule separations, and choosing the right phase is critical for achieving consistent retention, resolution, and peak shape. Agilent’s column platforms deliver industry-leading reproducibility and efficiency, with multiple bonded phases, pore sizes, and particle technologies engineered for both HPLC and UHPLC performance.

For expert guidance in selecting the ideal Agilent column for your application, contact Chrom Tech to connect with our chromatography specialists.

Explore HPLC Columns Page
Shop HPLC Columns
Explore all column families, modes, applications, and resources in the full pillar guide.

Agilent HPLC Column Categories

Explore genuine Agilent HPLC Columns

Shop InfinityLab Poroshell 120

Agilent Poroshell 120 Reversed Phase HPLC Columns

Features
  • Up to 50% less back pressure
  • Superior column performance
  • Narrow particle size distribution
  • Three particle sizes (1.9, 2.7, 4µm)
  • All-in-One Kits for Validation

Shop ZORBAX Eclipse Plus

The ultimate in performance for silica-based columns.

Features
  • Ideal column for method development
  • High level of performance—peak shape, efficiency, resolution, and lifetime
  • All sample types: acids, bases and neutrals

Shop ZORBAX Eclipse XDB

Four selectivity choices for method development optimization

Features
  • Good peak shape for basic, acidic and neutral compounds
  • Wide pH range (2–9)
  • Particle sizes from 1.8 to 7 µm
  • Long lifetime with XDB and double endcapping

Shop ZORBAX 80Å StableBond

Provide exceptional low-pH stability, long lifetime, and broad selectivity across six bonded phases.

Features
  • Longest column lifetime
  • Best reproducibility for low pH
  • Use at high temperature & low pH without degradation

Shop ZORBAX 80Å Extend-C18

Deliver high efficiency, excellent peak shape, and long life at high pH (up to 11.5), with bidentate bonding ideal for LC/MS peptide analysis.

Features
  • Bidentate bonding and double endcapping
  • Better peak shape than polymer-based compounds
  • High sensitivity for separations of peptides

Shop ZORBAX Bonus-RP Columns

Provide excellent peak shape for challenging basic compounds, offering unique reversed-phase selectivity, high aqueous compatibility, and strong low-pH stability through embedded-polar bonding and steric protection.

Features
  • For low/mid pH basic compounds
  • Unique reversed-phase selectivity
  • Novel bonding technology
  • Usable in 100% aqueous mobile phases

Shop ZORBAX Specialty Columns

For unique or complex separations not achievable with standard reversed-phase phases.

Features
  • Targeted selectivity for specialized or difficult separations
  • Optimized chemistries beyond standard reversed-phase phases
  • Reliable performance for unique method requirements

Shop Pursuit XRs Columns

Reliable performance across multiple particle sizes and phase chemistries.

Features
  • Multiple chemistries including USP L1, L7, and L11
  • Traditional and high-carbon-load bonding options
  • Wide range of dimensions, including preparative formats

Shop Polaris HPLC Columns

Deliver robust, aqueous-compatible performance for increasingly polar compounds.

Features
  • Designed for high-aqueous conditions
  • Improved retention and peak shape
  • Multiple C18 and C8 chemistries
Key Definitions ▸

Reversed-Phase Chromatography (RP)

The most widely used LC mode. Utilizes non-polar stationary phases such as C18 or C8 and polar mobile phases. Ideal for small molecules requiring predictable retention and high reproducibility.

Superficially Porous Particles (SPP)

Also called “core-shell” particles. Used in Agilent Poroshell 120 columns to deliver UHPLC-grade efficiency with reduced back-pressure, enabling faster and higher-resolution separations.

Fully Porous Particles (FPP)

Traditional particle design used in Agilent ZORBAX, Polaris, and Pursuit columns. Offers broad selectivity, robust durability, and compatibility across classic and modern LC methods.

Particle Size

A key factor influencing efficiency, pressure, and resolution. Smaller particles (≤2–3 µm) deliver UHPLC-level performance, while 3.5–5 µm particles are ideal for standard HPLC systems.

Stationary Phase

The bonded surface chemistry—such as C18, C8, Phenyl-Hexyl, CN, or Bonus-RP—that determines retention, selectivity, and overall chromatographic behavior.

Frequently Asked Questions ▸

What types of Agilent HPLC columns does Chrom Tech offer?

Chrom Tech offers the full Agilent portfolio, including InfinityLab Poroshell 120, ZORBAX Eclipse Plus and XDB, StableBond, Extend-C18, Bonus-RP, Polaris, and Pursuit XRs columns—optimized for small-molecule separations.

What is the difference between Poroshell and ZORBAX columns?

Poroshell 120 columns use superficially porous particles for higher efficiency and faster separations, while ZORBAX columns are fully porous and offer broad selectivity across many stationary phases and applications.

Can these Agilent columns be used for UHPLC?

Yes. Many Agilent columns—including Poroshell 120, ZORBAX Eclipse Plus, and Pursuit XRs—are rated for UHPLC platforms and deliver high efficiency and excellent resolution at elevated pressures.

Product List

1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
SKU Name LC Column Phase Particle Size Inner Diameter (ID) Length
SKU
822700-902
Name
822700-902 - SB-C18 2.1x50mm 1.8um
LC Column Phase
SB-C18
Particle Size
1.8 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
821125-924
Name
821125-924 - 300SB-C3 5u, 2.1 x 12.5mm 4/PK
LC Column Phase
300SB-C3
Particle Size
5 um
Inner Diameter (ID)
2.1 mm
Length
12.5 mm
SKU
699775-902
Name
699775-902 - Poroshell 120 EC-C18, 2.1 x 50 mm, 2.7 um
LC Column Phase
EC-C18
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
699775-408
Name
699775-408 - Poroshell 120 PFP,2.1x50mm, 2.7um
LC Column Phase
PFP
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
699975-502
Name
699975-502 - Poroshell HPH-C18,3x50mm, 2.7um
LC Column Phase
HPH-C18
Particle Size
2.7 um
Inner Diameter (ID)
3 mm
Length
50 mm
SKU
699975-302
Name
699975-302 - Poroshell 120 EC-C18 3x50mm 2.7um 600 bar
LC Column Phase
EC-C18
Particle Size
2.7 um
Inner Diameter (ID)
3 mm
Length
50 mm
SKU
689975-302
Name
689975-302 - Poroshell 120 SB-C18 3x50mm 2.7um 600 bar
LC Column Phase
SB-C18
Particle Size
2.7 um
Inner Diameter (ID)
3 mm
Length
50 mm
SKU
204205-308
Name
204205-308 - Altura ZORBAX Eclipse Plus C18 column 1.8μm, 2.1x50mm
LC Column Phase
Eclipse Plus C18
Particle Size
1.8 µm
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
204215-308
Name
204215-308 - Altura ZORBAX Eclipse Plus C18 column 1.8μm, 2.1x150mm
LC Column Phase
Eclipse Plus C18
Particle Size
1.8 µm
Inner Diameter (ID)
2.1 mm
Length
150 mm
SKU
204210-308
Name
204210-308 - Altura ZORBAX Eclipse Plus C18 column 1.8μm, 2.1x100mm
LC Column Phase
Eclipse Plus C18
Particle Size
1.8 µm
Inner Diameter (ID)
2.1 mm
Length
100 mm
SKU
217205-502
Name
217205-502 - Altura Poroshell HPH-C18 column 1.9μm, 2.1x50mm
LC Column Phase
HPH-C18
Particle Size
1.9 µm
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
217215-502
Name
217215-502 - Altura Poroshell HPH-C18 column 1.9μm 2.1x150mm
LC Column Phase
HPH-C18
Particle Size
1.9 µm
Inner Diameter (ID)
2.1 mm
Length
150 mm