Agilent BioHPLC

Agilent BioHPLC Columns for Proteins, Peptides & Biotherapeutics

Agilent BioHPLC columns provide high-resolution, high-reproducibility separations for proteins, peptides, glycans, monoclonal antibodies, viral particles, oligonucleotides, and other complex biomolecules. Engineered for precision and robustness, Agilent’s BioHPLC portfolio delivers exceptional performance across reversed-phase, size-exclusion, polymeric RP, and wide-pore LC/MS-compatible workflows used throughout biopharmaceutical development, QC, and advanced proteomics.

From Poroshell 300 and AdvanceBio RP-mAb columns designed for intact mAbs and large peptides, to AdvanceBio Glycan Mapping and Peptide Mapping solutions for detailed structural characterization, the BioHPLC family covers every major biotherapeutic analyte class. Polymer-based PLRP-S columns extend reversed-phase capability to large, highly hydrophobic macromolecules, while ZORBAX 300Å StableBond columns offer unmatched low-pH durability for peptide and protein separations.

For size-exclusion chromatography, Agilent’s offerings include AdvanceBio SEC HPLC Bio columns for mAbs and ADCs, ProSEC 300S for globular proteins, and LC/MS-compatible Bio SEC-3 columns for denaturing SEC workflows. Bio SEC-5 columns extend SEC analysis to very large biomolecules and viral-scale assemblies with pore sizes up to 2000 Å.

Whether you are characterizing aggregates, mapping peptides, quantifying glycans, or analyzing viral vectors and next-generation biotherapeutics, Agilent BioHPLC columns provide the proven stability, selectivity, and reproducibility required for modern bioanalytical workflows. For application guidance or assistance selecting the ideal column, contact Chrom Tech.

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Agilent BioHPLC Column Categories

Explore a full range of Agilent BioHPLC columns engineered for large-molecule and biopharmaceutical separations.

Key Definitions ▸
Wide-Pore Chromatography
Columns with pore sizes from 300 Å and above, designed to allow proteins, mAbs, and large peptides full access to the stationary phase for efficient separation.
Superficially Porous Particles (SPP)
Also known as core-shell particles, SPP media offer high efficiency with reduced back-pressure, ideal for peptide mapping, glycan analysis, and protein separations.
Fully Porous Particles (FPP)
Traditional LC particles offering broad selectivity and high loading capacity, commonly used for proteins, peptides, and biomolecule separations.
Size Exclusion Chromatography (SEC)
A separation mode based on molecular size, widely used for aggregate/fragment analysis of mAbs, ADCs, viral particles, and large biomolecules.
Hydrophilic, Low-Interaction Coating
A specialized surface used on modern SEC columns (e.g., AdvanceBio SEC) to prevent secondary interactions and ensure accurate aggregate quantitation—even for sticky samples.
High-pH Stable Bonding
Bidentate or sterically protected bonded phases allowing operation at elevated pH, enabling peptide, oligo, and protein separations that require high-pH mobile phases.
Polymeric Reversed-Phase Media
Fully polymeric, silanol-free particles like PLRP-S engineered for high chemical stability, excellent reproducibility, and broad applicability from peptides to large complexes.
Frequently Asked Questions ▸
Which Agilent BioHPLC column should I choose for intact monoclonal antibodies?
For intact mAbs and large fragments, Poroshell 300 and AdvanceBio RP-mAb columns provide the best resolution and reproducibility due to their wide-pore, high-efficiency designs.
What is the best column for glycan analysis?
AdvanceBio Glycan Mapping columns are purpose-built for fast, high-resolution glycan characterization and are available in both 2.7 µm SPP and 1.8 µm UHPLC formats.
Which BioHPLC columns are recommended for peptide mapping?
The InfinityLab Poroshell 120 AdvanceBio Peptide Mapping column is engineered for reproducible, batch-tested performance in complex peptide maps and LC/MS workflows.
Do I need a polymeric column for large or highly hydrophobic biomolecules?
Yes—fully polymeric PLRP-S columns are ideal for hydrophobic or large biomolecules because they offer exceptional chemical and thermal stability without silanols or bonded ligands.
Which SEC column should I use for aggregate and fragment analysis?
For mAbs, ADCs, and protein aggregates, AdvanceBio SEC 2.7 µm columns provide highly reproducible quantitation with minimal secondary interactions. For smaller proteins and LC/MS workflows, Bio SEC-3 is preferred.
Which size-exclusion column works best for large viral particles?
AdvanceBio SEC 500 Å and 1000 Å columns are optimized for AAVs and VLPs, offering robust, high-resolution separations for viral and large biomolecular assemblies.
Can these BioHPLC columns be used across multiple LC systems?
Yes—most Agilent BioHPLC columns (including Poroshell, AdvanceBio, PLRP-S, and SEC formats) are compatible with standard HPLC and UHPLC systems, ensuring easy method transfer and broad instrument compatibility.

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SKU Name Column Family LC Column Phase Particle Size Inner Diameter (ID)
SKU
PL1147-1501
Name
PL1147-1501 - ProSEC 300S Guard, 50x7.5mm
Column Family
Agilent ProSEC 300S
LC Column Phase
SEC
Particle Size
5 um
Inner Diameter (ID)
7.5 mm
SKU
693975-349
Name
693975-349 - AdvanceBio Peptide Plus 3.0 x 150 mm, 2.7 um
Column Family
AdvanceBio Peptide Plus
LC Column Phase
Peptide Plus
Particle Size
2.7 um
Inner Diameter (ID)
3 mm
SKU
693775-949
Name
693775-949 - AdvanceBio Peptide Plus 2.1 x 250 mm, 2.7 um
Column Family
AdvanceBio Peptide Plus
LC Column Phase
Peptide Plus
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
SKU
GKSB-503
Name
GKSB-503 - AdvanceBio 2-AB Glucose Homopolymer Stan
Column Family
LC Column Phase
Particle Size
Inner Diameter (ID)
SKU
GKE-5006D
Name
GKE-5006D - AdvanceBio N-Glycanase (PNGase F), >2.5
Column Family
LC Column Phase
Particle Size
Inner Diameter (ID)
SKU
PL1980-3250PK
Name
PL1980-3250PK - AdvanceBio SEC 120A 1.9um 2.1x150mm PEEK
Column Family
AdvanceBio SEC
LC Column Phase
SEC
Particle Size
1.9 um
Inner Diameter (ID)
2.1 mm
SKU
PL1980-3201PK
Name
PL1980-3201PK - AdvanceBio SEC 200A 1.9um 2.1x150mm PEEK
Column Family
AdvanceBio SEC
LC Column Phase
SEC
Particle Size
1.9 um
Inner Diameter (ID)
2.1 mm
SKU
PL1980-1250PK
Name
PL1980-1250PK - AdvanceBio SEC 120A 1.9um 2.1x50mm PEEK
Column Family
AdvanceBio SEC
LC Column Phase
SEC
Particle Size
1.9 um
Inner Diameter (ID)
2.1 mm
SKU
PL1980-1201PK
Name
PL1980-1201PK - AdvanceBio SEC 200A 1.9um 2.1x50mm PEEK
Column Family
AdvanceBio SEC
LC Column Phase
SEC
Particle Size
1.9 um
Inner Diameter (ID)
2.1 mm
SKU
863600-911
Name
863600-911 - AdvanceBio Peptide Mapping, 1.0 x 150 mm
Column Family
AdvanceBio Peptide Mapping
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
1 mm
SKU
679775-901
Name
679775-901 - AdvanceBio MS Spent Media 120A, 2.1x50mm
Column Family
AdvanceBio MS Spent Media
LC Column Phase
HILIC-Z
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
SKU
675775-901
Name
675775-901 - AdvanceBio MS Spent Media 120A,2.1x100mm
Column Family
AdvanceBio MS Spent Media
LC Column Phase
HILIC-Z
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm