Agilent BioHPLC

Agilent BioHPLC Columns for Proteins, Peptides & Biotherapeutics

Agilent BioHPLC columns provide high-resolution, high-reproducibility separations for proteins, peptides, glycans, monoclonal antibodies, viral particles, oligonucleotides, and other complex biomolecules. Engineered for precision and robustness, Agilent’s BioHPLC portfolio delivers exceptional performance across reversed-phase, size-exclusion, polymeric RP, and wide-pore LC/MS-compatible workflows used throughout biopharmaceutical development, QC, and advanced proteomics.

From Poroshell 300 and AdvanceBio RP-mAb columns designed for intact mAbs and large peptides, to AdvanceBio Glycan Mapping and Peptide Mapping solutions for detailed structural characterization, the BioHPLC family covers every major biotherapeutic analyte class. Polymer-based PLRP-S columns extend reversed-phase capability to large, highly hydrophobic macromolecules, while ZORBAX 300Å StableBond columns offer unmatched low-pH durability for peptide and protein separations.

For size-exclusion chromatography, Agilent’s offerings include AdvanceBio SEC HPLC Bio columns for mAbs and ADCs, ProSEC 300S for globular proteins, and LC/MS-compatible Bio SEC-3 columns for denaturing SEC workflows. Bio SEC-5 columns extend SEC analysis to very large biomolecules and viral-scale assemblies with pore sizes up to 2000 Å.

Whether you are characterizing aggregates, mapping peptides, quantifying glycans, or analyzing viral vectors and next-generation biotherapeutics, Agilent BioHPLC columns provide the proven stability, selectivity, and reproducibility required for modern bioanalytical workflows. For application guidance or assistance selecting the ideal column, contact Chrom Tech.

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Agilent BioHPLC Column Categories

Explore a full range of Agilent BioHPLC columns engineered for large-molecule and biopharmaceutical separations.

Key Definitions ▸
Wide-Pore Chromatography
Columns with pore sizes from 300 Å and above, designed to allow proteins, mAbs, and large peptides full access to the stationary phase for efficient separation.
Superficially Porous Particles (SPP)
Also known as core-shell particles, SPP media offer high efficiency with reduced back-pressure, ideal for peptide mapping, glycan analysis, and protein separations.
Fully Porous Particles (FPP)
Traditional LC particles offering broad selectivity and high loading capacity, commonly used for proteins, peptides, and biomolecule separations.
Size Exclusion Chromatography (SEC)
A separation mode based on molecular size, widely used for aggregate/fragment analysis of mAbs, ADCs, viral particles, and large biomolecules.
Hydrophilic, Low-Interaction Coating
A specialized surface used on modern SEC columns (e.g., AdvanceBio SEC) to prevent secondary interactions and ensure accurate aggregate quantitation—even for sticky samples.
High-pH Stable Bonding
Bidentate or sterically protected bonded phases allowing operation at elevated pH, enabling peptide, oligo, and protein separations that require high-pH mobile phases.
Polymeric Reversed-Phase Media
Fully polymeric, silanol-free particles like PLRP-S engineered for high chemical stability, excellent reproducibility, and broad applicability from peptides to large complexes.
Frequently Asked Questions ▸
Which Agilent BioHPLC column should I choose for intact monoclonal antibodies?
For intact mAbs and large fragments, Poroshell 300 and AdvanceBio RP-mAb columns provide the best resolution and reproducibility due to their wide-pore, high-efficiency designs.
What is the best column for glycan analysis?
AdvanceBio Glycan Mapping columns are purpose-built for fast, high-resolution glycan characterization and are available in both 2.7 µm SPP and 1.8 µm UHPLC formats.
Which BioHPLC columns are recommended for peptide mapping?
The InfinityLab Poroshell 120 AdvanceBio Peptide Mapping column is engineered for reproducible, batch-tested performance in complex peptide maps and LC/MS workflows.
Do I need a polymeric column for large or highly hydrophobic biomolecules?
Yes—fully polymeric PLRP-S columns are ideal for hydrophobic or large biomolecules because they offer exceptional chemical and thermal stability without silanols or bonded ligands.
Which SEC column should I use for aggregate and fragment analysis?
For mAbs, ADCs, and protein aggregates, AdvanceBio SEC 2.7 µm columns provide highly reproducible quantitation with minimal secondary interactions. For smaller proteins and LC/MS workflows, Bio SEC-3 is preferred.
Which size-exclusion column works best for large viral particles?
AdvanceBio SEC 500 Å and 1000 Å columns are optimized for AAVs and VLPs, offering robust, high-resolution separations for viral and large biomolecular assemblies.
Can these BioHPLC columns be used across multiple LC systems?
Yes—most Agilent BioHPLC columns (including Poroshell, AdvanceBio, PLRP-S, and SEC formats) are compatible with standard HPLC and UHPLC systems, ensuring easy method transfer and broad instrument compatibility.

Product List

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SKU Name Column Family LC Column Phase Particle Size Inner Diameter (ID)
SKU
821725-954
Name
821725-954 - UHPLC Guard, AdvanceBio Peptide Plus, 2.1 mm, 2.7 um, 3 pack
Column Family
AdvanceBio Peptide Plus
LC Column Phase
Peptide Plus
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
SKU
820750-941
Name
820750-941 - AdvanceBio Oligonucleotide, 4.6 mm, guard, 4um
Column Family
AdvanceBio Oligonucleotide
LC Column Phase
HPH-C18
Particle Size
4 um
Inner Diameter (ID)
4.6 mm
SKU
820750-940
Name
820750-940 - UHPLC Guard, AdvanceBio Peptide Plus, 4.6 mm, 2.7 um, 3 pack
Column Family
AdvanceBio Peptide Plus
LC Column Phase
Peptide Plus
Particle Size
2.7 um
Inner Diameter (ID)
4.6 mm
SKU
699971-702
Name
699971-702 - AdvanceBio Oligonucleotide, 4.6 x 50 mm, 4 um
Column Family
AdvanceBio Oligonucleotide
LC Column Phase
HPH-C18
Particle Size
4 um
Inner Diameter (ID)
4.6 mm
SKU
699775-949
Name
699775-949 - AdvanceBio Peptide Plus 2.1 x 50 mm, 2.7 um
Column Family
AdvanceBio Peptide Plus
LC Column Phase
Peptide Plus
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
SKU
695971-702
Name
695971-702 - AdvanceBio Oligonucleotide, 4.6 x 100 mm, 4 um
Column Family
AdvanceBio Oligonucleotide
LC Column Phase
HPH-C18
Particle Size
4 um
Inner Diameter (ID)
4.6 mm
SKU
695775-949K
Name
695775-949K - AdvanceBio Peptide Plus, 2.1 x 150 mm ,2.7 um, method validation kit
Column Family
AdvanceBio Peptide Plus
LC Column Phase
Peptide Plus
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
SKU
695775-949
Name
695775-949 - AdvanceBio Peptide Plus 2.1 x 150 mm, 2.7 um
Column Family
AdvanceBio Peptide Plus
LC Column Phase
Peptide Plus
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
SKU
693975-949
Name
693975-949 - AdvanceBio Peptide Plus 4.6 x 150 mm, 2.7 um
Column Family
AdvanceBio Peptide Plus
LC Column Phase
Peptide Plus
Particle Size
2.7 um
Inner Diameter (ID)
4.6 mm
SKU
693971-702
Name
693971-702 - AdvanceBio Oligonucleotide, 4.6 x 150 mm, 4 um
Column Family
AdvanceBio Oligonucleotide
LC Column Phase
HPH-C18
Particle Size
4 um
Inner Diameter (ID)
4.6 mm
SKU
685975-908K
Name
685975-908K - AdvanceBio HIC, 4.6 x 100 mm, 3.5 um, method validation kit
Column Family
LC Column Phase
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
SKU
685975-908
Name
685975-908 - AdvanceBio HIC, 4.6 x 100 mm, 3.5 um
Column Family
AdvanceBio HIC
LC Column Phase
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm