Agilent BioHPLC
Agilent BioHPLC Columns for Proteins, Peptides & Biotherapeutics
Agilent BioHPLC columns provide high-resolution, high-reproducibility separations for proteins, peptides, glycans, monoclonal antibodies, viral particles, oligonucleotides, and other complex biomolecules. Engineered for precision and robustness, Agilent’s BioHPLC portfolio delivers exceptional performance across reversed-phase, size-exclusion, polymeric RP, and wide-pore LC/MS-compatible workflows used throughout biopharmaceutical development, QC, and advanced proteomics.
From Poroshell 300 and AdvanceBio RP-mAb columns designed for intact mAbs and large peptides, to AdvanceBio Glycan Mapping and Peptide Mapping solutions for detailed structural characterization, the BioHPLC family covers every major biotherapeutic analyte class. Polymer-based PLRP-S columns extend reversed-phase capability to large, highly hydrophobic macromolecules, while ZORBAX 300Å StableBond columns offer unmatched low-pH durability for peptide and protein separations.
For size-exclusion chromatography, Agilent’s offerings include AdvanceBio SEC HPLC Bio columns for mAbs and ADCs, ProSEC 300S for globular proteins, and LC/MS-compatible Bio SEC-3 columns for denaturing SEC workflows. Bio SEC-5 columns extend SEC analysis to very large biomolecules and viral-scale assemblies with pore sizes up to 2000 Å.
Whether you are characterizing aggregates, mapping peptides, quantifying glycans, or analyzing viral vectors and next-generation biotherapeutics, Agilent BioHPLC columns provide the proven stability, selectivity, and reproducibility required for modern bioanalytical workflows. For application guidance or assistance selecting the ideal column, contact Chrom Tech.
Agilent BioHPLC Column Categories
Explore a full range of Agilent BioHPLC columns engineered for large-molecule and biopharmaceutical separations.
Shop Poroshell 300
Deliver high-efficiency separations of intact proteins and large peptide fragments.
- 5 µm, 300 Å SPP design ideal for large biomolecules
- StableBond chemistry for reliable performance
- Optimized for intact proteins and peptide fragments
Shop AdvanceBIo RP-mAB
Deliver higher resolution, faster run times, and reliable analysis of intact mAbs, fragments, and other large biomolecules.
- 450 Å wide-pore SPP design
- Multiple phase options (C4, SB-C8, Diphenyl)
- Extended lifetime with a robust packed bed and 2 µm inlet frit
Shop AdvanceBio Glycan Mapping
Deliver fast, high-resolution, and highly reproducible glycan analysis
- Rapid glycan maps
- High-resolution characterization of labeled and unlabeled glycans
- Available in 2.7 µm SPP and 1.8 µm UHPLC formats
Shop Poroshell Peptide Mapping
Provide highly reproducible, batch-tested performance to ensure reliable identification of key peptides in complex peptide maps.
- Batch-tested media
- Reliable identification of key peptides
- Designed for high reproducibility
Shop PLRP-S Columns
Use highly durable, fully polymeric particles with broad pore-size options (100–4000 Å) to deliver thermally and chemically stable, silanol-free separations for small molecules through large biomolecular complexes.
- Polymeric, silanol-free particles
- Wide pore-size range
- USP L21 compliant
Shop Zorbax 300A Stablebond
Provide highly reproducible, wide-pore reversed-phase separations for peptides and proteins, offering unmatched stability and long lifetime
- 300 Å wide-pore design
- Exceptional low-pH durability
- Four bonded phases + capillary/nano formats
Shop AdvanceBio SEC HPLC Bio Columns
Deliver size-exclusion separations for peptides, mAbs, ADCs, AAVs, and other biotherapeutics
- Highly reproducible SEC performance
- Hydrophilic, low-interaction surface minimizes secondary interactions
- Multiple pore and particle sizes (1.9µm & 2.7µm; 130–1000 Å)
Shop ProSEC 300S Columns
Provide a single-column, wide-range SEC solution for globular protein analysis.
- One-column solution for most globular protein separations
- Robust, low-bleed silica particles
- Enhanced sensitivity
Shop Bio SEC-3 Columns
Deliver high-efficiency size-exclusion separations compatible with SEC-MS workflows.
- SEC-MS compatible
- Stable performance for high and low salt conditions
- Scalable formats for analytical through preparative protein workflows
Shop Bio SEC-5 Columns
Provide high-resolution size-exclusion separations for large biomolecules, delivering exceptional stability, efficiency, and reproducibility.
- Exceptional resolution & peak capacity
- Neutral hydrophilic surface ensures high stability
- Up to 2000 Å pore sizes
Wide-Pore Chromatography
Columns with pore sizes from 300 Å and above, designed to allow proteins, mAbs, and large peptides full access to the stationary phase for efficient separation.
Superficially Porous Particles (SPP)
Also known as core-shell particles, SPP media offer high efficiency with reduced back-pressure, ideal for peptide mapping, glycan analysis, and protein separations.
Fully Porous Particles (FPP)
Traditional LC particles offering broad selectivity and high loading capacity, commonly used for proteins, peptides, and biomolecule separations.
Size Exclusion Chromatography (SEC)
A separation mode based on molecular size, widely used for aggregate/fragment analysis of mAbs, ADCs, viral particles, and large biomolecules.
Hydrophilic, Low-Interaction Coating
A specialized surface used on modern SEC columns (e.g., AdvanceBio SEC) to prevent secondary interactions and ensure accurate aggregate quantitation—even for sticky samples.
High-pH Stable Bonding
Bidentate or sterically protected bonded phases allowing operation at elevated pH, enabling peptide, oligo, and protein separations that require high-pH mobile phases.
Polymeric Reversed-Phase Media
Fully polymeric, silanol-free particles like PLRP-S engineered for high chemical stability, excellent reproducibility, and broad applicability from peptides to large complexes.
Which Agilent BioHPLC column should I choose for intact monoclonal antibodies?
For intact mAbs and large fragments, Poroshell 300 and AdvanceBio RP-mAb columns provide the best resolution and reproducibility due to their wide-pore, high-efficiency designs.
What is the best column for glycan analysis?
AdvanceBio Glycan Mapping columns are purpose-built for fast, high-resolution glycan characterization and are available in both 2.7 µm SPP and 1.8 µm UHPLC formats for maximum flexibility.
Which BioHPLC columns are recommended for peptide mapping?
The InfinityLab Poroshell 120 AdvanceBio Peptide Mapping column is engineered for reproducible, batch-tested performance in complex peptide maps and LC/MS workflows.
Do I need a polymeric column for large or highly hydrophobic biomolecules?
Yes—fully polymeric PLRP-S columns are ideal for hydrophobic or large biomolecules because they offer exceptional chemical and thermal stability without silanols or bonded ligands.
Which SEC column should I use for aggregate and fragment analysis?
For mAbs, ADCs, and protein aggregates, AdvanceBio SEC 2.7 µm columns provide highly reproducible quantitation with minimal secondary interactions. For smaller proteins and LC/MS workflows, Bio SEC-3 is preferred.
Which size-exclusion column works best for large viral particles?
AdvanceBio SEC 500 Å and 1000 Å columns are optimized for AAVs and VLPs, offering robust, high-resolution separations for viral and large biomolecular assemblies.
Can these BioHPLC columns be used across multiple LC systems?
Yes—most Agilent BioHPLC columns (including Poroshell, AdvanceBio, PLRP-S, and SEC formats) are compatible with standard HPLC and UHPLC systems, ensuring easy method transfer and broad instrument compatibility.
Product List
| SKU | Name | Column Family | LC Column Phase | Particle Size | Inner Diameter (ID) |
|---|---|---|---|---|---|
SKU
PL1580-3302
|
Name
PL1580-3302 - AdvanceBio SEC 1000 A, 2.7 um, 4.6x150mm
|
Column Family
AdvanceBio SEC
|
LC Column Phase
SEC
|
Particle Size
2.7 um
|
Inner Diameter (ID)
4.6 mm
|
SKU
PL1580-3325
|
Name
PL1580-3325 - AdvanceBio SEC 500 A, 2.7 um, 4.6x150mm
|
Column Family
AdvanceBio SEC
|
LC Column Phase
SEC
|
Particle Size
2.7 um
|
Inner Diameter (ID)
4.6 mm
|
SKU
PL1580-1302
|
Name
PL1580-1302 - AdvanceBioSEC 1000 A, 2.7 um, 4.6x50mm, guard
|
Column Family
AdvanceBio SEC
|
LC Column Phase
SEC
|
Particle Size
2.7 um
|
Inner Diameter (ID)
4.6 mm
|
SKU
PL1580-1325
|
Name
PL1580-1325 - AdvanceBio SEC 500 A, 2.7 um, 4.6x50mm, guard
|
Column Family
AdvanceBio SEC
|
LC Column Phase
SEC
|
Particle Size
2.7 um
|
Inner Diameter (ID)
4.6 mm
|
SKU
PL1580-5250K
|
Name
PL1580-5250K - AdvanceBio SEC 120 A method validation kit, 1.9 um, 4.6 x 300 mm
|
Column Family
AdvanceBio SEC
|
LC Column Phase
SEC
|
Particle Size
1.9 um
|
Inner Diameter (ID)
4.6 mm
|
SKU
PL1580-5250
|
Name
PL1580-5250 - AdvanceBio SEC 120 A column, 1.9 um, 4.6 x 300 mm
|
Column Family
AdvanceBio SEC
|
LC Column Phase
SEC
|
Particle Size
1.9 um
|
Inner Diameter (ID)
4.6 mm
|
SKU
PL1580-3250K
|
Name
PL1580-3250K - AdvanceBio SEC 120 A method validation kit, 1.9 um, 4.6 x 150 mm
|
Column Family
AdvanceBio SEC
|
LC Column Phase
SEC
|
Particle Size
1.9 um
|
Inner Diameter (ID)
4.6 mm
|
SKU
PL1580-3250
|
Name
PL1580-3250 - AdvanceBio SEC 120 A column, 1.9 um, 4.6 x 150 mm
|
Column Family
AdvanceBio SEC
|
LC Column Phase
SEC
|
Particle Size
1.9 um
|
Inner Diameter (ID)
4.6 mm
|
SKU
PL1580-1250
|
Name
PL1580-1250 - AdvanceBio SEC 120 A column, 1.9 um, 4.6 x 30 mm
|
Column Family
AdvanceBio SEC
|
LC Column Phase
SEC
|
Particle Size
1.9 um
|
Inner Diameter (ID)
4.6 mm
|
SKU
859750-913
|
Name
859750-913 - AdvanceBio Amide HILIC column, 2.1x150mm, 1.8um
|
Column Family
|
LC Column Phase
|
Particle Size
|
Inner Diameter (ID)
|
SKU
858750-913
|
Name
858750-913 - AdvanceBio Amide HILIC column, 2.1x100mm, 1.8um
|
Column Family
|
LC Column Phase
|
Particle Size
|
Inner Diameter (ID)
|
SKU
823750-952
|
Name
823750-952 - UHPLC Guard, AdvanceBio Peptide Plus, 3.0 mm, 2.7 um, 3 pack
|
Column Family
AdvanceBio Peptide Plus
|
LC Column Phase
Peptide Plus
|
Particle Size
2.7 um
|
Inner Diameter (ID)
3 mm
|