Zorbax 300A Stablebond
Agilent ZORBAX 300Å StableBond HPLC Columns
Agilent ZORBAX 300Å StableBond columns are engineered for high-performance separations of proteins, peptides, and other large biomolecules. Their wide-pore (300Å) design ensures that larger analytes fully access the bonded phase, enabling sharper peaks, improved recovery, and high-efficiency reversed-phase separations across complex biological samples.
Built on Agilent’s patented StableBond low-pH-resistant chemistry, these columns deliver exceptional stability and reproducibility when used with acidic mobile phases—particularly those containing TFA, formic acid, or acetic acid. This robustness makes them the preferred option for LC/MS workflows where low pH is required for sensitivity, ionization performance, and chromatographic resolution.
- Optimized bonded phases for peptide and protein selectivity (SB-C18, SB-C8, SB-Phenyl, SB-CN)
- Unmatched low-pH durability with TFA-rich mobile phases—ideal for peptide mapping and protein profiling
- Excellent reproducibility for regulated and high-precision LC/MS workflows
- Available in analytical, capillary, and nano formats for proteomics, bio-pharma, and LC/MS applications
Whether you are analyzing intact proteins, peptide digests, biotherapeutics, or high-molecular-weight biomolecules, ZORBAX 300Å StableBond columns provide the stability, selectivity, and reliability essential for modern bio-separations.
For assistance selecting the right phase or geometry for your workflow, contact Chrom Tech.
Wide-Pore Columns (300 Å)
Large-pore media designed for peptides, proteins, and large biomolecules. The 300 Å pore size allows high–molecular-weight analytes to fully access the bonded phase for improved efficiency and peak shape.
StableBond (SB) Chemistry
Agilent’s patented low-pH-resistant bonding technology designed for extreme durability with TFA and other acidic mobile phases. The sterically protected siloxane bond resists hydrolysis under harsh conditions.
TFA-Containing Mobile Phases
Mobile phases with trifluoroacetic acid, commonly used in peptide and protein separations for improved ion-pairing and chromatographic performance. These conditions are typically aggressive toward silica—making StableBond chemistry essential for long column life.
LC/MS-Compatible Acid Modifiers
Additives such as formic acid and acetic acid that improve electrospray ionization efficiency and preserve instrument cleanliness while maintaining stable reversed-phase retention.
Bonded Phase Selectivity
The range of hydrophobic and aromatic selectivities offered by SB-C18, SB-C8, SB-C3, and SB-Phenyl phases, enabling tailored separations for peptides, proteins, and other large biomolecules.
Why use 300Å StableBond columns for proteins and peptides?
Large molecules—such as intact proteins, peptides, and biotherapeutic fragments— require wide-pore (300Å) media to fully access the stationary phase. ZORBAX 300Å StableBond columns deliver the pore architecture and durability needed for reproducible biomolecule separations at low pH.
Can 300Å StableBond columns withstand low-pH mobile phases like TFA?
Yes. The patented StableBond chemistry provides unmatched durability in acidic conditions—especially TFA-containing mobile phases commonly used for peptide mapping and LC/MS workflows—ensuring long lifetime and consistent retention.
What bonded phases are available in the 300Å StableBond family?
The 300Å StableBond series includes multiple bonded phases, allowing users to fine-tune selectivity for peptides and proteins. Options include SB-C18, SB-C8, SB-Phenyl, and SB-CN for a broad range of reversed-phase biomolecule applications.
Are 300Å StableBond columns compatible with LC/MS workflows?
Absolutely. These columns excel in LC/MS analyses of peptides and proteins, and support low-pH modifiers such as formic acid and acetic acid when TFA-free conditions are required for improved ionization efficiency.
Product List
| SKU | Name | LC Column Phase | Particle Size | Inner Diameter (ID) | Length |
|---|---|---|---|---|---|
SKU
821125-918
|
Name
821125-918 - 300SB-C8 Nar Bor Grd Car 2.1x12.5mm 4PK
|
LC Column Phase
300SB-C8
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
13 mm
|
SKU
820675-124
|
Name
820675-124 - 300SB-C3 Prep Relinc Grd Col 9.4x15 2/PK
|
LC Column Phase
300SB-C3
|
Particle Size
7 um
|
Inner Diameter (ID)
9.4 mm
|
Length
15 mm
|
SKU
5185-5920
|
Name
5185-5920 - StableBond Guard, 1.0x17mm, 5um, 3/pk
|
LC Column Phase
SB-C18
|
Particle Size
5 um
|
Inner Diameter (ID)
1 mm
|
Length
17 mm
|