The Benefits of Glass Well Plate Inserts
96 well plates are a standard consumable in high-throughput analytical laboratories that utilize mass spectrometry. Most commonly, we see polypropylene 96 deep well plates 2mL square or 1mL round 96 well plates. In some instances, polypropylene is not an ideal material to come in contact with your sample or extraction solvents. Other times, customers want to automate their extraction process and use a 96 well plate format but are concerned with evaporation. For either of these circumstances, we recommend a 96 well plate with glass inserts.
Using Glass Inserts for Better Precision and Lower CV Values
To improve assay robustness, one scientist presented a poster at MSACL in 2019 highlighting the impact of our glass inserts in 96 well plates:
Insulin detemir concentration, with its C14 myristic acid fatty chain, would decrease over time following elution from the antibody tips. This was due to the hydrophobic peptides sticking to plastic collection plates. By changing from plastic collection plates to glass inserts the precision decreased at least five fold for detemir and all other analogs, into an acceptable range of <10% CV. (Girtman, et al.)
Using Glass Inserts for the Analysis of Fatty Acid Methyl Esters (FAMEs)
ABSTRACT: Fatty acids (FAs) are essential for building complex lipids, posttranslational modifications, and energetics. FAs can be imported from extracellular sources or synthesized by cells. The analysis of fatty acid methyl esters (FAMEs) by gas chromatography–mass spectrometry (GC–MS) allows for the quantitative analysis of long-chain and very-long-chain fatty acid content of cells. When coupled with isotopic labeling, this approach can elucidate the synthetic pathways being engaged by the cells, and the relative contribution of synthesis and import to maintain lipid content. Here, we describe a method for total cellular fatty acid analysis in macrophages. (Williams, et al.)
Williams et al. describe the use of glass inserts in an aluminum 96-well block, which allows a molded PTFE-lined silicone mat to be pressed into the inserts. An aluminum cover plate (with 96 holes) is then secured over the mat with screws. The additional aluminum cover puts consistent downward pressure on the PTFE-lined silicone mat and minimizes the chance of evaporation during extraction. Using glass inserts in a 96-well plate format for this type of assay allows for automation and improves the analysis.
If you are looking to automate your analytical assay and use 96 well plates and are concerned with your sample coming in contact with plastic or evaporation, please reach out to Chrom Tech to discuss 96 well plate glass insert options.
Girtman, Adam, Grebe, Stefan, Singh, Ravinder. “The Challenges Associated with the Development of a Clinically Viable Quantitative Insulin Analog Assay.” MSACL 2019 US : Girtman, www.msacl.org/view_abstract/MSACL_2019_US.php?id=928.
Williams K.J., Bensinger S.J. (2020) “Cellular Fatty Acid Analysis in Macrophage Using Stable Isotope Labeling.” In: Mishra S. (eds) Immunometabolism. Methods in Molecular Biology, vol 2184. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0802-9_4