Rtx-65TG

Rtx-65TG column

Restek Rtx-65TG GC Columns

Restek Rtx-65TG GC columns are specifically engineered for triglyceride (TG) and fatty acid profiling, delivering the enhanced selectivity needed to separate complex mixtures of lipid species. Featuring a highly polar 65% diphenyl, 35% dimethylpolysiloxane stationary phase, Rtx-65TG provides exceptionally strong aromatic and dipole–dipole interaction—ideal for resolving triglyceride positional isomers, saturated and unsaturated fatty acids, and structurally similar lipid components.

This specialized phase excels in applications where subtle structural or positional differences create co-elution challenges on conventional GC columns. Food chemistry laboratories, edible oil producers, biodiesel researchers, and lipid analysis facilities rely on Rtx-65TG for its ability to effectively separate C18:1 isomers, trans-fatty acid species, long-chain triglycerides, and a broad range of thermally stable lipid-related analytes.

With strong thermal stability, low bleed, and Restek’s proven column deactivation, Rtx-65TG maintains excellent peak symmetry and reproducibility—even under extended high-temperature programs required for triglyceride separations. The phase delivers clean baselines and reliable quantitation in GC-FID and GC-MS workflows.

As an authorized Restek distributor, Chrom Tech supplies Rtx-65TG in multiple dimensions tailored for high-resolution lipid and triglyceride profiling workflows, supporting both routine quality testing and advanced food, nutritional, and biodiesel research.

Key Definitions
65% Diphenyl Polysiloxane Phase
A highly polar GC phase offering exceptional aromatic and polar interaction strength, essential for resolving triglycerides and fatty acid isomers.
Triglyceride Profiling
The analytical separation of triacylglycerols based on fatty acid composition and positional isomerism—an application requiring phases with strong polarity and thermal stability.
Positional Isomer Resolution
The ability to distinguish compounds that share the same molecular formula but differ in the location of functional groups—critical for lipid and fatty acid speciation.
High-Temperature Lipid Analysis
Rtx-65TG supports extended oven programs required to elute long-chain triglycerides, while maintaining low bleed and sharp peak shapes.
FAME Selectivity
Although optimized for triglycerides, Rtx-65TG also provides strong separation for FAMEs, including cis/trans and chain-length isomers.
Frequently Asked Questions
What applications are best suited for the Rtx-65TG column?
Rtx-65TG is ideal for triglyceride profiling, fatty acid speciation, trans-fat analysis, edible oil characterization, and biodiesel-related lipid separations.
How does Rtx-65TG differ from the standard Rtx-65 column?
While both use a 65% diphenyl phase, Rtx-65TG is optimized specifically for high-temperature lipid chromatography and the resolution of triglyceride and fatty acid isomers.
Can Rtx-65TG be used for GC-MS workflows?
Yes. The column’s low bleed and thermal robustness make it suitable for GC-MS analysis, especially for high-boiling lipid compounds.
Does Rtx-65TG require special temperature programming?
For triglyceride analysis, extended high-temperature holds are common, and Rtx-65TG is engineered to withstand these conditions while maintaining strong performance and low bleed.