Raptor LC Columns

Raptor Biphenyl combines superficially porous particle (SPP) core-shell technology with a phenyl (L11) stationary phase to deliver enhanced selectivity for structurally similar and early-eluting compounds. It is particularly effective for analytes that are difficult to resolve on traditional C18 or other phenyl chemistries.

The biphenyl ligand increases retention for polar, dipolar, unsaturated, and conjugated solutes through π–π interactions and unique electronic selectivity. This makes it especially valuable for bioanalytical testing, pharmaceutical impurities, drug metabolites, and LC-MS workflows where improved retention can reduce ion suppression and simplify mobile phase composition.

Key Specifications

• Stationary Phase Category: Phenyl (L11)
• Ligand Type: Biphenyl
• Particle Sizes: 1.8 µm, 2.7 µm, 5 µm (core-shell SPP silica)
• Pore Size: 90 Å
• Carbon Load: 7% (1.8 µm), 7% (2.7 µm), 5% (5 µm)
• End-Capped: Yes
• Surface Area: 125 m²/g (1.8 µm); 130 m²/g (2.7 µm); 100 m²/g (5 µm)

Recommended Usage

• pH Range: 2.0–8.0
• Maximum Temperature: 80 °C
• Maximum Pressure: 1034 bar / 15,000 psi (1.8 µm); 600 bar / 8,700 psi (2.7 µm); 400 bar / 5,800 psi (5 µm)

For maximum lifetime, recommended maximum pressure for 1.8 µm particles is 830 bar / 12,000 psi.

When to Choose Biphenyl

• Limited selectivity is observed on a C18 column.
• Increased retention is needed for hydrophilic aromatics.
• Enhanced sensitivity and selectivity are required for LC-MS analyses.
• Improved separation of dipolar or conjugated compounds is needed.

Raptor ARC-18 is a sterically protected C18 phase designed specifically for demanding LC-MS/MS applications that use harsh, low-pH mobile phases. Built on superficially porous particle (SPP) core-shell silica, it delivers high efficiency, fast separations, and exceptional acid stability.

Unlike conventional C18 columns that may lose retention or peak quality under strongly acidic conditions, ARC-18 maintains a well-balanced retention profile and consistent peak shape. It is especially effective for large, multiclass analyte lists where reproducibility, sensitivity, and durability are critical.

Key Specifications

• Stationary Phase Category: C18, Octadecylsilane (L1)
• Ligand Type: Sterically Protected C18
• Particle Sizes: 1.8 µm, 2.7 µm, 5 µm (core-shell SPP silica)
• Pore Size: 90 Å
• Carbon Load: 7% (1.8 µm), 7% (2.7 µm), 5% (5 µm)
• End-Capped: No
• Surface Area: 125 m²/g (1.8 µm); 130 m²/g (2.7 µm); 100 m²/g (5 µm)

Recommended Usage

• pH Range: 1.0–8.0
• Maximum Temperature: 80 °C
• Maximum Pressure: 1034 bar / 15,000 psi (1.8 µm); 600 bar / 8,700 psi (2.7 µm); 400 bar / 5,800 psi (5 µm)

For maximum lifetime, recommended maximum pressure for 1.8 µm particles is 830 bar / 12,000 psi.

When to Choose ARC-18

• Strongly acidic (pH 1–3) mobile phases are required.
• Analyzing large, multiclass compound lists by LC-MS/MS.
• Improved durability is needed under harsh low-pH conditions.
• Consistent peak shape and retention are critical for sensitive detection.

Raptor C18 is a traditional end-capped C18 phase built on superficially porous particle (SPP) core-shell silica for fast, high-efficiency separations. It delivers the highest hydrophobic retention of any Raptor phase, making it an excellent general-purpose choice for reversed-phase chromatography.

Designed for broad applicability, Raptor C18 provides consistent data quality across food safety, environmental, pharmaceutical, and bioanalytical workflows. Its balanced retention profile and compatibility with moderately acidic to neutral mobile phases make it ideal for laboratories seeking speed, durability, and reproducibility.

Key Specifications

• Stationary Phase Category: C18, Octadecylsilane (L1)
• Ligand Type: End-Capped C18
• Particle Sizes: 1.8 µm, 2.7 µm, 5 µm (core-shell SPP silica)
• Pore Size: 90 Å
• Carbon Load: 9% (1.8 µm), 7% (2.7 µm), 5% (5 µm)
• End-Capped: Yes
• Surface Area: 125 m²/g (1.8 µm); 130 m²/g (2.7 µm); 100 m²/g (5 µm)

Recommended Usage

• pH Range: 2.0–8.0
• Maximum Temperature: 80 °C
• Maximum Pressure: 1034 bar / 15,000 psi (1.8 µm); 600 bar / 8,700 psi (2.7 µm); 400 bar / 5,800 psi (5 µm)

For maximum lifetime, recommended maximum pressure for 1.8 µm particles is 830 bar / 12,000 psi.

When to Choose C18

• You need a general-purpose column for reversed-phase chromatography.
• Higher retention of hydrophobic compounds is required.
• Broad method compatibility across applications and matrices is desired.
• Reliable, reproducible performance is critical for routine analysis.

Raptor Polar X is a purpose-built phase for the analysis of highly polar compounds that are difficult to retain using traditional reversed-phase chromatography. Built on 2.7 µm superficially porous particle (SPP) core-shell silica, Polar X combines HILIC and ion-exchange retention mechanisms on a single ligand for enhanced selectivity and flexibility.

This dual-mode chemistry allows analysts to switch between HILIC and ion-exchange behavior with simple mobile phase adjustments—eliminating the need for derivatization or complex ion-pairing strategies. The result is reliable retention of acidic, basic, neutral, and permanently charged polar analytes with improved sensitivity and faster method development.

Key Specifications

• Phase Category: Proprietary Polar Selectivity
• Retention Mechanism: Combined HILIC & Ion-Exchange
• Particle Size: 2.7 µm (core-shell SPP silica)
• Pore Size: 90 Å
• Surface Area: 130 m²/g
• End-Cap: Proprietary
• Carbon Load: Proprietary

Recommended Usage

• pH Range: 2.0–8.0
• Maximum Temperature: 60 °C
• Maximum Pressure: 600 bar / 8,700 psi

Ideal Applications

• LC-MS/MS analysis of highly polar compounds.
• Multi-residue pesticide methods (e.g., QuPPe workflows).
• Retention of neutral, acidic, basic, or permanently charged polar analytes.
• Situations where reversed-phase chromatography provides inadequate retention.

Storage Guidance

When not in use, store Raptor Polar X columns in 100% acetonitrile. If operated with buffered mobile phases, flush thoroughly with 50:50 water:acetonitrile before filling with acetonitrile for storage.

Raptor FluoroPhenyl combines superficially porous particle (SPP) core-shell technology with a pentafluorophenyl propyl (L43) stationary phase to provide orthogonal selectivity to traditional C18 columns. This phase retains hydrophobic, polar, and aromatic compounds while offering enhanced interactions for charged bases and structurally complex analytes.

Designed for versatility, Raptor FluoroPhenyl can operate in both reversed-phase and HILIC modes depending on mobile phase composition. Its unique electronic and dipole interactions improve retention and selectivity for basic and hydrophilic compounds that may exhibit limited resolution on conventional C18 phases.

Key Specifications

• Stationary Phase Category: Pentafluorophenyl Propyl (L43)
• Ligand Type: FluoroPhenyl
• Particle Sizes: 1.8 µm, 2.7 µm, 5 µm (core-shell SPP silica)
• Pore Size: 90 Å
• Carbon Load: 4% (1.8 µm), 4% (2.7 µm), 3% (5 µm)
• End-Capped: No
• Surface Area: 125 m²/g (1.8 µm); 130 m²/g (2.7 µm); 100 m²/g (5 µm)

Recommended Usage

• pH Range: 2.0–8.0
• Maximum Temperature: 80 °C
• Maximum Pressure: 1034 bar / 15,000 psi (1.8 µm); 600 bar / 8,700 psi (2.7 µm); 400 bar / 5,800 psi (5 µm)

For maximum lifetime, recommended maximum pressure for 1.8 µm particles is 830 bar / 12,000 psi.

When to Choose FluoroPhenyl

• Limited selectivity or retention is observed on a C18 for basic compounds.
• Additional retention of hydrophilic or aromatic compounds is required.
• Orthogonal selectivity to C18 is needed for method development.
• Improved sensitivity and selectivity are desired in LC-MS analyses.

Raptor C8 is a conventional monomeric octylsilane (L7) phase built on 2.7 µm superficially porous particle (SPP) core-shell silica. With a shorter alkyl chain than C18, it provides reduced hydrophobic retention and faster analysis times while maintaining excellent peak shape and reproducibility.

Offering similar selectivity to C18 but with less retention of highly nonpolar analytes, Raptor C8 is well suited for laboratories seeking balanced performance across cannabis, food safety, clinical, and environmental applications. Its versatility supports compounds ranging from acidic to slightly basic.

Key Specifications

• Stationary Phase Category: C8, Octylsilane (L7)
• Ligand Type: C8
• Particle Size: 2.7 µm (core-shell SPP silica)
• Pore Size: 90 Å
• Carbon Load: 4.6%
• End-Capped: Yes
• Surface Area: 130 m²/g

Recommended Usage

• pH Range: 2.0–8.0
• Maximum Temperature: 80 °C
• Maximum Pressure: 600 bar / 8,700 psi

When to Choose C8

• You want faster run times than a C18 can provide.
• Highly nonpolar compounds are over-retained on C18.
• You need balanced retention with improved peak shape for basic analytes.
• Broad applicability across multiple industries is required.

Raptor HILIC-Si is a bare silica (L3) phase designed for hydrophilic interaction liquid chromatography (HILIC). Built on 2.7 µm superficially porous particle (SPP) core-shell silica, it delivers increased retention of small, highly polar compounds without the need for ion-pairing reagents.

The HILIC-Si phase enables efficient separation of hydrophilic analytes while maintaining compatibility with both HPLC and UHPLC systems. Its core-shell design provides high efficiency, improved sensitivity, and faster run times compared to conventional 5 µm silica columns.

Key Specifications

• Stationary Phase Category: Bare Silica (L3)
• Ligand Type: None (Unbonded Silica)
• Particle Size: 2.7 µm (core-shell SPP silica)
• Pore Size: 90 Å
• Surface Area: 130 m²/g

Recommended Usage

• pH Range: 1.0–8.0
• Maximum Temperature: 80 °C
• Maximum Pressure: 600 bar / 8,700 psi (2.7 µm)

When to Choose HILIC-Si

• Increased retention of small polar compounds is required.
• You want to avoid ion-pairing reagents in LC-MS methods.
• Hydrophilic compounds show poor retention on reversed-phase columns.
• Enhanced sensitivity and selectivity are needed for polar analytes.

Raptor EtG/EtS is a proprietary phase specifically engineered for accurate and reliable analysis of ethyl glucuronide (EtG) and ethyl sulfate (EtS) by LC-MS/MS. Built on 2.7 µm superficially porous particle (SPP) core-shell silica, this column delivers high efficiency, strong retention, and fast run times for high-throughput toxicology workflows.

Designed to resolve EtG and EtS from challenging biological matrix components, the Raptor EtG/EtS column supports dilute-and-shoot methods with rapid analysis times—often in as little as four minutes—while maintaining consistent peak shape and reproducibility injection after injection.

Key Specifications

• Phase Category: Proprietary
• Ligand Type: Proprietary
• Particle Size: 2.7 µm (core-shell SPP silica)
• Pore Size: 90 Å
• Surface Area: 130 m²/g
• End-Cap: Proprietary
• Carbon Load: Proprietary

Recommended Usage

• pH Range: 2.0–8.0
• Maximum Temperature: 40 °C
• Maximum Pressure: 600 bar / 8,700 psi

Ideal Applications

• High-throughput LC-MS/MS analysis of EtG and EtS.
• Toxicology and clinical testing laboratories.
• Dilute-and-shoot sample preparation workflows.
• Methods requiring low detection limits and minimal matrix interference.

When to Choose Raptor EtG/EtS

• Other phases cannot adequately resolve EtG and EtS from matrix components.
• High sample throughput with short run times is required.
• Improved retention and reproducibility are needed for EtG/EtS analysis.