Quantitatively Determining 15 Endogenous Steroids in Human Serum

As people age, both women and men may encounter health issues that can be addressed through hormone replacement therapy, according to Women’s Healthcare Associates. However, hormones are metabolized differently in each individual, which adds complexity to treating patients effectively while minimizing the risk of diseases like cancer, dementia, and bone density loss. To monitor the health of patients and the success of hormone replacement therapies, it is crucial for researchers to accurately measure endogenous steroid levels.

Traditionally, liquid-liquid extraction has been the method of choice for isolating endogenous steroids from biological samples such as serum and plasma for analysis. However, this conventional technique is time-consuming, labor-intensive, and often yields low recovery rates. Agilent has shown a quantitative determination of 15 endogenous steroids in human serum by LC/MS/MS, using their Chem Elut S supported liquid extraction. Supported liquid extraction produces higher recovery, and the special synthetic media provides better consistency and higher water holding capacity than standard diatomaceous earth.

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