Acclaim

Thermo Scientificâ„¢ Acclaim (U)HPLC Columns

Thermo Scientificâ„¢ Acclaimâ„¢ (U)HPLC columns are engineered for complex separations that demand enhanced selectivity, higher resolving power, and robust quantitative performance. Built on high surface area, fully porous silica platforms, the Acclaim family delivers exceptional reproducibility and method reliability across analytical UHPLC and HPLC systems.

Designed to solve difficult analytical challenges, Acclaim columns provide a broad portfolio of rare and specialty chemistries—from traditional C18 and C8 phases to mixed-mode, HILIC, ion-exchange, and application-specific formats. This diversity enables optimized retention mechanisms for hydrophobic, polar, ionic, and structurally similar analytes.

Acclaim columns are widely used for LC-MS workflows, pharmaceutical analysis, environmental testing, food safety, biomolecule separations, and specialty regulatory methods. Whether modernizing a legacy HPLC method or developing a new UHPLC workflow, Acclaim columns offer scalable particle sizes and dimensions for seamless method transfer and long-term reproducibility.

Performance Advantages

  • High surface area silica for increased retention and improved resolution of complex mixtures
  • Expanded selectivity portfolio including Polar Advantage, Phenyl-1, C30, mixed-mode, Trinity, HILIC-10, and specialty phases
  • Fully porous particle technology optimized for analytical UHPLC and HPLC platforms
  • LC-MS compatible options for high-sensitivity detection workflows
  • Scalable column dimensions for method development and transfer flexibility

Available Chemistries

The Acclaim portfolio includes reversed-phase, polar-embedded, aromatic, HILIC, mixed-mode, ion-exchange, and specialty application phases:

  • Reversed-Phase: 120 C18, 120 C8, 300 C18, C30
  • Polar-Embedded: Polar Advantage (PA), Polar Advantage II (PA2)
  • Aromatic Selectivity: Phenyl-1
  • HILIC: HILIC-10, Mixed-Mode HILIC-1
  • Mixed-Mode / Ion Exchange: Trinity P1, Trinity P2, Mixed-Mode WAX-1, Mixed-Mode WCX-1
  • Specialty Phases: Organic Acid, Surfactant Plus, Explosives E2, Carbamate, Carbonyl C18, AmG C18, Trinity Q1, SEC formats

Applications

  • Pharmaceutical method development and validation
  • Complex impurity profiling
  • Environmental contaminant analysis
  • Food and beverage testing
  • Biomolecule and peptide separations
  • Regulatory and EPA method workflows

Chrom Tech is an authorized distributor of Thermo Scientificâ„¢ chromatography products and stocks a wide selection of Acclaim (U)HPLC columns for fast shipment and technical support.

Phase
Particle (µm)
Format
Length (mm)
ID (mm)
 Part Number
120 C182.2UHPLC column302.1071400
Polar Advantage II2.2UHPLC column302.1071402
120 C182.2UHPLC column502.1068981
Polar Advantage II2.2UHPLC column502.1068989
120 C182.2UHPLC column503.0071605
120 C182.2UHPLC column753.0075697
120 C182.2UHPLC column1002.1068982
120 C82.2UHPLC column1002.1072616
Polar Advantage II2.2UHPLC column1002.1068990
120 C182.2UHPLC column1003.0071604
Polar Advantage II2.2UHPLC column1003.0071607
120 C182.2UHPLC column1502.1071399
Polar Advantage II2.2UHPLC column1502.1071401
120 C182.2UHPLC column2502.1074812
Polar Advantage II2.2UHPLC column2502.1074814
120 C182.2Vanquish column1502.1071399-V
Polar Advantage II2.2Vanquish column1502.1071401-V
120 C182.2Vanquish column2502.1074812-V
Polar Advantage II2.2Vanquish column2502.1074814-V
Carbonyl C182.2UHPLC column1002.1077972
Carbonyl C182.2UHPLC column1003.0077974
Carbonyl C182.2UHPLC column1502.1077973
Explosives E22.2UHPLC column1502.1076226
120 C183.0HPLC column303.0066272
Polar Advantage II3.0HPLC column303.0066276
120 C183.0HPLC column502.1059128
120 C83.0HPLC column502.1059122
Polar Advantage II3.0HPLC column502.1077999
C303.0HPLC column502.1078666
Polar Advantage II3.0HPLC column503.0068973
Mixed-Mode HILIC-13.0HPLC column503.0071912
Mixed-Mode WAX-13.0HPLC column503.0071908
120 C183.0HPLC column504.6059131
Polar Advantage II3.0HPLC column504.6063189
C303.0HPLC column504.6078661
120 C183.0HPLC column753.0066273
Polar Advantage II3.0HPLC column753.0066277
120 C183.0HPLC column1002.1059129
120 C83.0HPLC column1002.1059123
Polar Advantage3.0HPLC column1002.1061316
Polar Advantage II3.0HPLC column1002.1077998
C303.0HPLC column1002.1078665
120 C183.0HPLC column1003.0076186
Polar Advantage II3.0HPLC column1003.0078000
C303.0HPLC column1003.0078662
120 C183.0HPLC column1004.6059132
Polar Advantage II3.0HPLC column1004.6078001
C303.0HPLC column1004.6078660
120 C183.0HPLC column1502.1059130
120 C83.0HPLC column1502.1059124
Polar Advantage3.0HPLC column1502.1061317
Polar Advantage II3.0HPLC column1502.1063187
Phenyl-13.0HPLC column1502.1071971
C303.0HPLC column1502.1075725
HILIC-103.0HPLC column1502.1074259
Mixed-Mode HILIC-13.0HPLC column1502.1070091
Mixed-Mode WAX-13.0HPLC column1502.1070089
120 C183.0HPLC column1503.0063691
120 C83.0HPLC column1503.0068970
Polar Advantage3.0HPLC column1503.0063693
Polar Advantage II3.0HPLC column1503.0063705
Phenyl-13.0HPLC column1503.0071970
C303.0HPLC column1503.0075724
Mixed-Mode HILIC-13.0HPLC column1503.0070090
Mixed-Mode WAX-13.0HPLC column1503.0070088
120 C183.0HPLC column1504.6059133
120 C83.0HPLC column1504.6059127
Polar Advantage3.0HPLC column1504.6061318
Polar Advantage II3.0HPLC column1504.6063191
Phenyl-13.0HPLC column1504.6071969
C303.0HPLC column1504.6075723
120 C183.0HPLC column2502.1076187
Polar Advantage II3.0HPLC column2502.1077997
C303.0HPLC column2502.1078664
120 C183.0HPLC column2503.0070077
Polar Advantage3.0HPLC column2503.0070079
Polar Advantage II3.0HPLC column2503.0070080
C303.0HPLC column2503.0075726
120 C185.0HPLC column502.1059142
120 C85.0HPLC column502.1059134
120 C185.0HPLC column504.6059146
120 C85.0HPLC column504.6059138
Polar Advantage5.0HPLC column504.6061319
120 C185.0HPLC column1002.1059143
120 C185.0HPLC column1004.6059147
120 C85.0HPLC column1004.6059139
120 C185.0HPLC column1502.1059144
120 C85.0HPLC column1502.1059136
Mixed-Mode HILIC-15.0HPLC column1502.1066847
Mixed-Mode WAX-15.0HPLC column1502.1067084
120 C185.0HPLC column1504.6059148
120 C85.0HPLC column1504.6059140
Polar Advantage5.0HPLC column1504.6061320
Polar Advantage II5.0HPLC column1504.6063197
C305.0HPLC column1504.6075719
Mixed-Mode HILIC-15.0HPLC column1504.6066843
Mixed-Mode WAX-15.0HPLC column1504.6064984
120 C185.0HPLC column2502.1059145
120 C185.0HPLC column2504.6059149
120 C85.0HPLC column2504.6059141
Polar Advantage5.0HPLC column2504.6061321
Polar Advantage II5.0HPLC column2504.6063199
Phenyl-15.0HPLC column2504.6079697
C305.0HPLC column2504.6075718
Mixed-Mode HILIC-15.0HPLC column2504.6066844
Mixed-Mode WAX-15.0HPLC column2504.6064985
Trinity P13.0HPLC column502.1075565
Trinity P23.0HPLC column502.1085431
300 C183.0HPLC column502.1060263
Trinity Q13.0HPLC column502.1083242
Mixed-Mode WCX-13.0HPLC column503.0071910
Trinity P13.0HPLC column503.0071388
Trinity P23.0HPLC column503.0085433
Trinity Q13.0HPLC column503.0083241
300 C183.0HPLC column504.6060265
Trinity P13.0HPLC column1002.1071389
Trinity P23.0HPLC column1002.1085432
Surfactant Plus3.0HPLC column1002.1078955
Trinity Q13.0HPLC column1002.1079717
Trinity P13.0HPLC column1003.0071387
Trinity P23.0HPLC column1003.0085434
Surfactant Plus3.0HPLC column1003.0078952
Trinity Q13.0HPLC column1003.0079715
Mixed-Mode WCX-13.0HPLC column1502.1070093
Trinity P13.0HPLC column1502.1075564
300 C183.0HPLC column1502.1060264
Organic Acid3.0HPLC column1502.1070087
Surfactant3.0HPLC column1502.1070085
Surfactant Plus3.0HPLC column1502.1078954
Carbonyl C183.0HPLC column1502.1079011
AmG C183.0HPLC column1502.1088753
Mixed-Mode WCX-13.0HPLC column1503.0070092
Trinity P13.0HPLC column1503.0075563
300 C183.0HPLC column1503.0063684
Organic Acid3.0HPLC column1503.0070086
Surfactant3.0HPLC column1503.0070084
Surfactant Plus3.0HPLC column1503.0078951
Carbonyl C183.0HPLC column1503.0079010
Carbamate C183.0HPLC column1503.0072926
Explosives E23.0HPLC column1503.0070082
AmG C183.0HPLC column1503.0088755
300 C183.0HPLC column1504.6060266
Surfactant Plus3.0HPLC column1504.6078950
AmG C183.0HPLC column1504.6088757
Surfactant Plus3.0HPLC column2502.1078953
Carbonyl C183.0HPLC column2503.0079009
Explosives E23.0HPLC column2503.0070081
Mixed-Mode WCX-15.0HPLC column1502.1068371
Surfactant5.0HPLC column1502.1068123
Organic Acid5.0HPLC column1504.0062903
Mixed-Mode WCX-15.0HPLC column1504.6068353
Surfactant5.0HPLC column1504.6063201
SEC-3005.0HPLC column1507.8079726
Organic Acid5.0HPLC column2504.0062902
Mixed-Mode WCX-15.0HPLC column3004.6068352
Surfactant5.0HPLC column3004.6063203
Carbonyl C185.0HPLC column3004.6083214
Carbamate C185.0HPLC column3004.6072924
Explosives E25.0HPLC column3004.6064309
SEC-3005.0HPLC column3004.6079723
SEC-3005.0HPLC column3007.8079725
SEC-10007.0HPLC column1507.8079722
SEC-10007.0HPLC column3004.6079724
SEC-10007.0HPLC column3007.8079721

Guard Cartridges & Columns

Phase
Particle (µm)
Format
Length (mm)
ID (mm)
 Part Number
HILIC-103.0Guard cartridge102.1074263
Phenyl-13.0Guard cartridge103.0071974
Phenyl-13.0Guard cartridge104.6071973
120 C185.0Guard cartridge102.1069689
120 C85.0Guard cartridge102.1069688
Polar Advantage5.0Guard cartridge102.1069691
Polar Advantage II5.0Guard cartridge102.1069692
C305.0Guard cartridge102.1075722
Mixed-Mode HILIC-15.0Guard cartridge102.1069694
Mixed-Mode WAX-15.0Guard cartridge102.1069686
120 C185.0Guard cartridge103.0071981
120 C85.0Guard cartridge103.0071979
Polar Advantage5.0Guard cartridge103.0071983
Polar Advantage II5.0Guard cartridge103.0071985
C305.0Guard cartridge103.0075721
Mixed-Mode HILIC-15.0Guard cartridge103.0071913
Mixed-Mode WAX-15.0Guard cartridge103.0071909
120 C185.0Guard cartridge104.6069695
120 C85.0Guard cartridge104.6069696
Polar Advantage5.0Guard cartridge104.6069698
Polar Advantage II5.0Guard cartridge104.6069699
C305.0Guard cartridge104.6075720
Mixed-Mode HILIC-15.0Guard cartridge104.6069706
Mixed-Mode WAX-15.0Guard cartridge104.6069704
Trinity P13.0Guard cartridge102.1071391
Trinity P23.0Guard cartridge102.1085435
Carbonyl C183.0Guard cartridge102.1079012
AmG C183.0Guard cartridge102.1088754
Trinity P13.0Guard cartridge103.0071390
Trinity P23.0Guard cartridge103.0085436
Carbonyl C183.0Guard cartridge103.0079013
AmG C183.0Guard cartridge103.0088756
Carbonyl C183.0Guard cartridge104.6079014
AmG C183.0Guard cartridge104.6088758
300 C185.0Guard cartridge102.1069690
Surfactant5.0Guard cartridge102.1069693
Surfactant Plus5.0Guard cartridge102.1078960
Trinity Q15.0Guard cartridge102.1083244
Mixed-Mode WCX-15.0Guard cartridge103.0071911
Organic Acid5.0Guard cartridge103.0071987
Surfactant5.0Guard cartridge103.0071991
Surfactant Plus5.0Guard cartridge103.0078959
Explosives E25.0Guard cartridge103.0071989
Trinity Q15.0Guard cartridge103.0079719
Mixed-Mode WCX-15.0Guard cartridge104.6069705
300 C185.0Guard cartridge104.6069697
Organic Acid5.0Guard cartridge104.6069700
Surfactant5.0Guard cartridge104.6069701
Surfactant Plus5.0Guard cartridge104.6082773
Explosives E25.0Guard cartridge104.6069703
SEC-3005.0Guard column334.6082740
Trinity Q17.0Guard column334.6082739
Key Definitions â–¶
Acclaimâ„¢ (U)HPLC columns
A family of Thermo Scientific fully porous silica-based LC columns designed to deliver reproducible separations across UHPLC and HPLC systems using a wide range of stationary-phase chemistries.
Stationary phase
The bonded chemical chemistry on the column packing (e.g., C18, C8, Phenyl, HILIC, ion-exchange) that controls analyte retention and selectivity during separation.
Fully porous particles
Silica particles with pore networks throughout the entire particle volume, providing high surface area for retention and broad method versatility in analytical LC.
Particle size (µm)
The average diameter of the column packing particles. Smaller particles generally increase efficiency and resolution but also increase backpressure; larger particles reduce pressure and may be preferred for conventional HPLC.
Column ID (internal diameter)
The inner diameter of the column (e.g., 2.1 mm, 3.0 mm, 4.6 mm). Smaller IDs reduce solvent consumption and improve LC-MS sensitivity; larger IDs can increase loadability for UV methods.
Column length
The packed bed length (mm). Longer columns generally increase resolution, while shorter columns support faster run times and higher throughput.
Selectivity
How a stationary phase differentiates between analytes, influencing relative retention and peak separation. Changing phase chemistry is often the fastest way to resolve co-eluting compounds.
HILIC (Hydrophilic Interaction LC)
A retention mode that enhances separation of polar analytes using a polar stationary phase with high organic mobile phases, commonly used for sugars, nucleotides, and polar metabolites.
Mixed-mode chromatography
Columns designed to provide more than one retention mechanism (e.g., reversed-phase plus ion-exchange), improving separation of complex mixtures containing both neutral and ionic analytes.
LC-MS compatibility
Suitability for mass spectrometry detection—typically requiring low-bleed, reproducible phases and mobile-phase conditions that support stable ionization and sensitive detection.
Frequently Asked Questions â–¶
What applications are Acclaimâ„¢ columns best suited for?
Acclaimâ„¢ (U)HPLC columns are designed for versatile analytical separations including pharmaceuticals, environmental testing, food analysis, peptides, polar compounds, and ionizable analytes. The wide range of stationary phases supports reversed-phase, HILIC, and ion-exchange methods.
How do I choose the correct particle size?
Smaller particle sizes (e.g., sub-2 µm) provide higher efficiency and sharper peaks but require UHPLC systems capable of higher pressure. Larger particles (3 µm or 5 µm) are compatible with conventional HPLC systems and may offer lower backpressure with robust performance.
What is the difference between C18, C8, and Phenyl phases?
C18 phases provide strong hydrophobic retention and broad applicability. C8 phases offer slightly reduced retention and faster elution. Phenyl phases introduce π-π interactions, improving selectivity for aromatic compounds and structurally related analytes.
When should I use HILIC or ion-exchange chemistries?
HILIC is ideal for highly polar compounds that show limited retention in reversed-phase systems. Ion-exchange or mixed-mode phases are preferred for strongly ionic analytes where charge-based retention improves resolution and method robustness.
How do column length and internal diameter affect performance?
Longer columns increase resolving power but extend run time. Shorter columns enable faster analyses. Smaller internal diameters reduce solvent consumption and improve LC-MS sensitivity, while larger diameters may support higher sample loading for UV-based detection.
Are Acclaim columns compatible with LC-MS systems?
Yes. Many Acclaim stationary phases are designed for low bleed and reproducible performance, making them suitable for LC-MS applications when used with appropriate volatile mobile phases and system configurations.