Hypersil Gold

Thermo Scientific Hypersil GOLD (U)HPLC Columns

  • High-efficiency, fully porous silica for fast, high-resolution separations
  • Excellent peak shape and sensitivity for complex mixtures and method development
  • Scalable across particle sizes (UHPLC to HPLC) and a wide phase portfolio

Thermo Scientific™ Hypersil GOLD™ columns are a go-to starting point when you need high-efficiency separations, tight peaks, and dependable performance for routine or demanding reversed-phase workflows. Built on ultrapure silica, Hypersil GOLD delivers excellent peak shape and higher peak capacity than many competitive columns—supporting fast run times, strong sensitivity, and reproducible results over time.

The platform is designed to scale cleanly across common analytical formats and particle sizes—from 1.9 µm (UHPLC) to 3.0 µm and 5.0 µm (HPLC)—so you can modernize methods or match system pressure limits without sacrificing chromatographic integrity. Hypersil GOLD also provides broad selectivity options beyond traditional C18, helping you tune retention and resolve challenging pairs by switching phase chemistry instead of rewriting your method from scratch.

Why labs choose Hypersil GOLD

  • Fast, predictable separations: excellent efficiency and peak shape for low-to-moderate complexity samples.
  • Method flexibility: a broad phase lineup supports selectivity changes for difficult analytes and matrices.
  • Scalability: select the particle size and format that best fits UHPLC or standard HPLC platforms.

Available Hypersil GOLD phase options

Hypersil GOLD is offered in a wide range of stationary phases to match retention needs and analyte chemistry, including: GOLD (C18), C8, C4, Phenyl, aQ (aqueous-compatible), PFP, CN (cyano), Amino, AX, SAX, Silica, HILIC, and Peptide. Select phases are also available in Vanquish UHPLC formats for high-pressure compatible workflows.

Particle size (µm)
Column type
Length (mm)
ID (mm)
Phase
Part number
1.9 µm — UHPLC column
1.9UHPLC column202.1GOLD25002-022130
1.9UHPLC column301.0GOLD25002-031030
1.9UHPLC column302.1GOLD25002-032130
1.9UHPLC column302.1aQ25302-032130
1.9UHPLC column501.0GOLD25002-051030
1.9UHPLC column501.0aQ25302-051030
1.9UHPLC column502.1GOLD25002-052130
1.9UHPLC column502.1C825202-052130
1.9UHPLC column502.1C425502-052130
1.9UHPLC column502.1aQ25302-052130
1.9UHPLC column502.1PFP25402-052130
1.9UHPLC column502.1Phenyl25902-052130
1.9UHPLC column502.1CN25802-052130
1.9UHPLC column502.1Amino25702-052130
1.9UHPLC column502.1Silica25102-052130
1.9UHPLC column502.1HILIC26502-052130
1.9UHPLC column503.0GOLD25002-053030
1.9UHPLC column503.0aQ25302-053030
1.9UHPLC column503.0PFP25402-053030
1.9UHPLC column504.6GOLD25002-054630
1.9UHPLC column504.6aQ25302-054630
1.9UHPLC column1001.0GOLD25002-101030
1.9UHPLC column1001.0C825202-101030
1.9UHPLC column1001.0aQ25302-101030
1.9UHPLC column1002.1GOLD25002-102130
1.9UHPLC column1002.1C825202-102130
1.9UHPLC column1002.1C425502-102130
1.9UHPLC column1002.1aQ25302-102130
1.9UHPLC column1002.1PFP25402-102130
1.9UHPLC column1002.1Phenyl25902-102130
1.9UHPLC column1002.1CN25802-102130
1.9UHPLC column1002.1Amino25702-102130
1.9UHPLC column1002.1SAX26302-102130
1.9UHPLC column1002.1Silica25102-102130
1.9UHPLC column1002.1HILIC26502-102130
1.9UHPLC column1003.0GOLD25002-103030
1.9UHPLC column1003.0aQ25302-103030
1.9UHPLC column1003.0PFP25402-103030
1.9UHPLC column1502.1GOLD25002-152130
1.9UHPLC column1502.1C825202-152130
1.9UHPLC column1502.1C425502-152130
1.9UHPLC column1502.1aQ25302-152130
1.9UHPLC column1502.1PFP25402-152130
1.9UHPLC column1502.1Phenyl25902-152130
1.9UHPLC column1502.1CN25802-152130
1.9UHPLC column1502.1Amino25702-152130
1.9UHPLC column1502.1SAX26302-152130
1.9UHPLC column1502.1Silica25102-152130
1.9UHPLC column1502.1HILIC26502-152130
1.9UHPLC column2002.1GOLD25002-202130
1.9UHPLC column2002.1aQ25302-202130
1.9UHPLC column2002.1Amino25702-202130
1.9UHPLC column2002.1Silica25102-202130
3 µm — HPLC column
3HPLC column302.1GOLD25003-032130
3HPLC column302.1aQ25303-032130
3HPLC column303.0GOLD25003-033030
3HPLC column303.0C825203-033030
3HPLC column304.6GOLD25003-034630
3HPLC column304.6C825203-034630
3HPLC column502.1GOLD25003-052130
3HPLC column502.1C825203-052130
3HPLC column502.1C425503-052130
3HPLC column502.1aQ25303-052130
3HPLC column502.1PFP25403-052130
3HPLC column502.1SAX26303-052130
3HPLC column502.1HILIC26503-052130
3HPLC column503.0GOLD25003-053030
3HPLC column503.0C825203-053030
3HPLC column503.0aQ25303-053030
3HPLC column504.0GOLD25003-054030
3HPLC column504.0aQ25303-054030
3HPLC column504.6GOLD25003-054630
3HPLC column504.6C825203-054630
3HPLC column504.6aQ25303-054630
3HPLC column1001.0GOLD25003-101030
3HPLC column1002.1GOLD25003-102130
3HPLC column1002.1Peptide26002-102130
3HPLC column1002.1C825203-102130
3HPLC column1002.1aQ25303-102130
3HPLC column1002.1PFP25403-102130
3HPLC column1002.1Phenyl25903-102130
3HPLC column1002.1AX26103-102130
3HPLC column1002.1HILIC26503-102130
3HPLC column1003.0GOLD25003-103030
3HPLC column1003.0C825203-103030
3HPLC column1003.0aQ25303-103030
3HPLC column1003.0PFP25403-103030
3HPLC column1003.0CN25803-103030
3HPLC column1003.0HILIC26503-103030
3HPLC column1004.0GOLD25003-104030
3HPLC column1004.6GOLD25003-104630
3HPLC column1004.6C825203-104630
3HPLC column1004.6aQ25303-104630
3HPLC column1004.6PFP25403-104630
3HPLC column1004.6Phenyl25903-104630
3HPLC column1004.6Amino25703-104630
3HPLC column1501.0GOLD25003-151030
3HPLC column1501.0aQ25303-151030
3HPLC column1501.0HILIC26503-151030
3HPLC column1502.1GOLD25003-152130
3HPLC column1502.1Peptide26002-152130
3HPLC column1502.1C825203-152130
3HPLC column1502.1aQ25303-152130
3HPLC column1502.1PFP25403-152130
3HPLC column1502.1Amino25703-152130
3HPLC column1502.1HILIC26503-152130
3HPLC column1503.0GOLD25003-153030
3HPLC column1503.0C825203-153030
3HPLC column1503.0aQ25303-153030
3HPLC column1503.0PFP25403-153030
3HPLC column1503.0Amino25703-153030
3HPLC column1503.0Silica25103-153030
3HPLC column1503.0HILIC26503-153030
3HPLC column1504.6GOLD25003-154630
3HPLC column1504.6C825203-154630
3HPLC column1504.6C425503-154630
3HPLC column1504.6aQ25303-154630
3HPLC column1504.6PFP25403-154630
3HPLC column1504.6Phenyl25903-154630
3HPLC column1504.6CN25803-154630
3HPLC column1504.6Amino25703-154630
3HPLC column1504.6SAX26303-154630
3HPLC column1504.6Silica25103-154630
3HPLC column1504.6HILIC26503-154630
5 µm — HPLC column
5HPLC column502.1GOLD25005-052130
5HPLC column502.1C825205-052130
5HPLC column502.1aQ25305-052130
5HPLC column502.1PFP25405-052130
5HPLC column502.1Phenyl25905-052130
5HPLC column502.1HILIC26505-052130
5HPLC column503.0GOLD25005-053030
5HPLC column503.0aQ25305-053030
5HPLC column504.6GOLD25005-054630
5HPLC column504.6C825205-054630
5HPLC column504.6aQ25305-054630
5HPLC column1002.1GOLD25005-102130
5HPLC column1002.1C825205-102130
5HPLC column1002.1aQ25305-102130
5HPLC column1002.1PFP25405-102130
5HPLC column1002.1Phenyl25905-102130
5HPLC column1002.1HILIC26505-102130
5HPLC column1003.0GOLD25005-103030
5HPLC column1003.0HILIC26505-103030
5HPLC column1502.1GOLD25005-152130
5HPLC column1502.1C825205-152130
5HPLC column1502.1aQ25305-152130
5HPLC column1502.1PFP25405-152130
5HPLC column1503.0GOLD25005-153030
5HPLC column1503.0aQ25305-153030
5HPLC column1504.0GOLD25005-154030
5HPLC column2502.1GOLD25005-252130
5HPLC column2502.1aQ25305-252130
5HPLC column2502.1PFP25405-252130
5HPLC column2503.0GOLD25005-253030
5HPLC column2504.0GOLD25005-254030
5HPLC column2504.0C825205-254030
5HPLC column2504.0CN25805-254030
5HPLC column2504.0Silica25105-254030
5HPLC column2504.6GOLD25005-254630
5HPLC column2504.6C825205-254630
5HPLC column2504.6C425505-254630
5HPLC column2504.6PFP25405-254630
5HPLC column2504.6Phenyl25905-254630
5HPLC column2504.6CN25805-254630
5HPLC column2504.6Amino25705-254630
5HPLC column2504.6SAX26305-254630
5HPLC column2504.6Silica25105-254630
5HPLC column2504.6HILIC26505-254630

HYPERSIL GOLD DROP-IN GUARDS

Particle size (µm)
Length (mm)
ID (mm)
Phase
Part number
3 µm — Drop-In-Guard (4/pk)
3101.0GOLD25003-011001
3102.1GOLD25003-012101
3103.0GOLD25003-013001
3104.0/4.6GOLD25003-014001
3101.0C825203-011001
3102.1C825203-012101
3103.0C825203-013001
3104.0/4.6C825203-014001
3102.1C425503-012101
3103.0C425503-013001
3101.0aQ25303-011001
3102.1aQ25303-012101
3103.0aQ25303-013001
3104.0/4.6aQ25303-014001
3101.0PFP25403-011001
3102.1PFP25403-012101
3103.0PFP25403-013001
3104.0/4.6PFP25403-014001
3102.1Phenyl25903-012101
3103.0Phenyl25903-013001
3104.0/4.6Phenyl25903-014001
3101.0CN25803-011001
3102.1CN25803-012101
3104.0/4.6CN25803-014001
3103.0Amino25703-013001
3104.0/4.6Amino25703-014001
3102.1AX26103-012101
3104.0/4.6Silica25103-014001
3101.0HILIC26503-011001
3102.1HILIC26503-012101
5 µm — Drop-In-Guard (4/pk)
5102.1GOLD25005-012101
5103.0GOLD25005-013001
5104.0/4.6GOLD25005-014001
5102.1C825205-012101
5103.0C825205-013001
5104.0/4.6C825205-014001
5102.1aQ25305-012101
5103.0aQ25305-013001
5104.0/4.6aQ25305-014001
5102.1PFP25405-012101
5104.0/4.6PFP25405-014001
5102.1Phenyl25905-012101
5104.0/4.6CN25805-014001
5104.0/4.6Amino25705-014001
5103.0SAX26305-013001
5104.0/4.6SAX26305-014001
5104.0/4.6HILIC26505-014001
Key Definitions ▶
Fully Porous Silica
A traditional silica particle structure where the entire particle contributes to separation. Fully porous particles provide high surface area and strong retention, making them ideal for a wide range of reversed-phase and mixed-mode applications.
Particle Size (µm)
The diameter of individual silica particles in the column packing. Smaller particles (e.g., 1.9 µm) deliver higher efficiency and faster separations but require higher system pressure, while larger particles (3–5 µm) are optimized for conventional HPLC.
Reversed-Phase Chromatography
A separation mode using a nonpolar stationary phase (such as C18) and a polar mobile phase. Analytes are separated based on hydrophobic interactions, making this the most widely used technique in analytical LC.
Selectivity
The relative retention differences between analytes on a given stationary phase. Changing column chemistry (e.g., C18 to Phenyl) can improve resolution without altering the mobile phase or gradient.
Peak Capacity
A measure of how many analytes can be resolved in a chromatographic run. Higher peak capacity improves the ability to separate complex mixtures and closely eluting compounds.
Frequently Asked Questions ▶
What is Hypersil GOLD best used for?
Hypersil GOLD is a strong “default” choice for reversed-phase LC when you want high efficiency, excellent peak shape, and dependable reproducibility. It’s commonly used for routine QC, method development, and complex mixture separations where sensitivity and resolution matter.
How do I choose between 1.9 µm, 3.0 µm, and 5.0 µm particle sizes?
Use 1.9 µm when you need the highest efficiency and fastest separations and your system supports UHPLC pressures. Choose 3.0 µm for a balance of speed and pressure on many modern LC systems, and 5.0 µm for robust, lower-pressure HPLC methods and instruments with more limited pressure capability.
Can I scale a method from UHPLC to HPLC using Hypersil GOLD?
Yes. Hypersil GOLD phases are offered across multiple particle sizes and common analytical formats, making method scaling straightforward. In most cases, you can keep the same stationary phase chemistry and adjust flow rate and gradient conditions to maintain comparable selectivity and retention.
Which Hypersil GOLD phase should I start with?
Start with Hypersil GOLD (C18) for general reversed-phase methods. If you need better retention for polar analytes, consider aQ (aqueous-compatible) or HILIC. For aromatic/selectivity changes, try Phenyl or PFP. For peptides or biomolecules, look at phases designed for those workflows (such as Peptide or wider-pore options).
How do I extend Hypersil GOLD column life?
Filter samples when possible, use an in-line filter or guard column for dirty matrices, and follow good mobile-phase hygiene (fresh solvents, clean glassware, and appropriate aqueous/organic ratios). For methods prone to fouling, a matched guard cartridge can significantly reduce particulate and strongly retained contaminant loading on the analytical column.