ZORBAX Specialty Columns

Agilent ZORBAX Specialty Columns

Agilent ZORBAX Specialty Columns provide targeted selectivity solutions for complex or application-specific separations that traditional reversed-phase chemistries cannot resolve. Engineered on high-purity silica and backed by Agilent’s industry-leading bonding technologies, these phases deliver exceptional efficiency, reproducibility, and robustness across challenging matrices and method conditions.

The ZORBAX Eclipse PAH column is optimized for polycyclic aromatic hydrocarbon (PAH) analysis, offering enhanced aromatic selectivity and sharp peak shape for environmental and food-safety workflows. ZORBAX HILIC Plus provides reliable retention of highly polar and hydrophilic analytes without derivatization, making it ideal for metabolites, sugars, and small polar molecules.

The ZORBAX NH2 amino-bonded phase supports both carbohydrate profiling and anion-exchange mechanisms, giving laboratories dual-mode versatility for sugars, organic acids, and polar compounds. For bioanalytical and small-molecule separations requiring superior peak shape and high efficiency, ZORBAX Rx columns feature extra-stable bonded phases designed for reproducible, high-purity separations under a wide range of pH and mobile-phase conditions.

Chrom Tech supplies the complete portfolio of Agilent ZORBAX Specialty Columns, helping you match the right selectivity to your method—whether you’re solving complex aromatic separations, retaining ultra-polar compounds, analyzing carbohydrates, or optimizing bioanalytical workflows.

Key Definitions

PAHs (Polycyclic Aromatic Hydrocarbons)

A class of multi-ring aromatic compounds commonly monitored in environmental, food, and materials analysis. Specialized phases like ZORBAX Eclipse PAH are engineered for their strong π-π interactions and high selectivity toward PAH isomers.

HILIC (Hydrophilic Interaction Liquid Chromatography)

A separation mode optimized for polar analytes using high organic mobile phases. ZORBAX HILIC Plus columns provide improved retention, sharp peak shape, and better MS compatibility for hydrophilic compounds.

Amino (NH2) Columns

Silica-based columns functionalized with primary amino groups used for carbohydrate analysis, normal-phase separations, and LC/MS-friendly retention of sugars and polar metabolites. ZORBAX NH2 provides stable retention with excellent reproducibility.

Rx Silica Bonding

A specialized bonding and endcapping technology used in ZORBAX Rx columns to improve peak symmetry, reduce silanol activity, and enhance reproducibility for peptides, bases, and biomolecules.

Frequently Asked Questions

What makes Agilent ZORBAX Specialty Columns unique?

ZORBAX Specialty Columns deliver focused selectivity for complex analytical challenges—from PAH separations to carbohydrate analysis—using refined silica, specialized bonding chemistries, and optimized particle design for enhanced reproducibility and stability.

When should I choose ZORBAX Eclipse PAH columns?

Eclipse PAH columns are ideal for resolving structurally similar polycyclic aromatic hydrocarbons with high efficiency and outstanding selectivity, making them suited for environmental and food safety workflows.

What applications benefit most from ZORBAX HILIC Plus columns?

ZORBAX HILIC Plus columns excel at retaining small, highly polar compounds—including metabolites, sugars, and hydrophilic pharmaceuticals—using HILIC mechanisms compatible with LC/MS workflows.

How is ZORBAX NH2 used in carbohydrate analysis?

ZORBAX NH2 columns use normal-phase and weak anion-exchange mechanisms to deliver excellent retention and separation of sugars, sugar alcohols, and other carbohydrates—offering robust performance in food, biotech, and QC workflows.

What makes ZORBAX Rx columns ideal for biomolecule analysis?

ZORBAX Rx columns feature ultrapure silica and controlled bonding optimized for peptides, proteins, and other biomolecules—providing high recovery, excellent peak shape, and compatibility with a wide pH range.

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SKU Name LC Column Phase Particle Size Inner Diameter (ID) Length
SKU
877150-106
Name
877150-106 - PrepHT,Zorbax,SB-C8,21.2x150mm,7uCrt
LC Column Phase
Rx-C8
Particle Size
7 um
Inner Diameter (ID)
21.2 mm
Length
150 mm
SKU
861967-902
Name
861967-902 - Rx-C18, 4.6x100mm 3.5um
LC Column Phase
Rx-C18
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
100 mm
SKU
861967-302
Name
861967-302 - Rx-C18 3.0 x 100mm 3.5u
LC Column Phase
Rx-C18
Particle Size
3.5 um
Inner Diameter (ID)
3 mm
Length
100 mm
SKU
863967-902
Name
863967-902 - Rx-C18 Rapid Res HPLC Col 4.6 x 150
LC Column Phase
Rx-C18
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
150 mm
SKU
863967-302
Name
863967-302 - Rx-C18 3.0x150mm 3.5u
LC Column Phase
Rx-C18
Particle Size
3.5 um
Inner Diameter (ID)
3 mm
Length
150 mm
SKU
870150-906
Name
870150-906 - PrepHT,ZORBAX,SB-C8,21.2x150mm,5u Crt
LC Column Phase
SB-C8
Particle Size
5 um
Inner Diameter (ID)
21.2 mm
Length
150 mm
SKU
870100-906
Name
870100-906 - PrepHT,ZORBAX,SB-C8,21.2x100mm,5u Crt
LC Column Phase
Rx-C8
Particle Size
5 um
Inner Diameter (ID)
21.2 mm
Length
100 mm
SKU
866967-902
Name
866967-902 - Rx-C18 Rapid Res HPLC Col 4.6 x 75
LC Column Phase
Rx-C18
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
75 mm
SKU
870050-906
Name
870050-906 - PrepHT,ZORBAX, SB-C8,21.2x50mm, 5u Crt
LC Column Phase
Rx-C8
Particle Size
5 um
Inner Diameter (ID)
21.2 mm
Length
50 mm
SKU
861767-902
Name
861767-902 - Rx-C18, 2.1x100mm 3.5um
LC Column Phase
Rx-C18
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
100 mm
SKU
860700-708
Name
860700-708 - ZORBAX NH2, 2.1 x 50mm, 5um
LC Column Phase
Original NH2
Particle Size
5 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
840140-901
Name
840140-901 - Semiprep Guard Hardware Kit
LC Column Phase
Particle Size
Inner Diameter (ID)
9.4 mm
Length
15 mm