HP-5ms Ultra Inert

HP-5ms Ultra Inert

HP-5ms Ultra Inert (UI) GC Columns

Agilent J&W HP-5ms Ultra Inert GC columns are engineered to deliver superior inertness, extremely low bleed, and exceptional reproducibility for high-sensitivity GC/MS applications. Built on a (5%-phenyl)-methylpolysiloxane stationary phase, the HP-5ms UI column maintains predictable non-polar selectivity while undergoing Agilent’s most advanced Ultra Inert deactivation process to minimize active sites inside the column.

Each column is individually tested for inertness using Agilent’s Ultra Inert probe mixture to verify excellent peak shapes and high analyte recovery—even for challenging active compounds such as amines, phenols, pesticides, and other acidic or basic analytes. This enhanced inertness ensures minimal adsorption and reduced chemical interaction within the column, supporting lower detection limits and more accurate quantification.

With its extremely low bleed profile, HP-5ms UI provides cleaner baselines, improved signal-to-noise ratios, and excellent mass spectral integrity—critical advantages for modern high-resolution MS and trace-level analyses. The bonded and cross-linked design supports solvent-rinsing and high-temperature operation, ensuring durability and long-term stability across demanding workflows.

HP-5ms Ultra Inert is also an excellent match for USP Phase G27, making it a strong choice for regulated methods and validated applications. Its combination of stability, inertness, and low-bleed performance makes it ideal for environmental monitoring, forensics, pharmaceutical impurity profiling, petrochemical testing, and general GC/MS analyses where data integrity and sensitivity are essential.

Key Definitions â–¸
Ultra Inert (UI) Deactivation
Agilent’s highest-level surface deactivation process designed to minimize active sites inside the GC column. UI technology enhances peak shape, analyte recovery, and consistency, especially for acidic, basic, or otherwise reactive compounds.
(5%-Phenyl)-Methylpolysiloxane
A widely used non-polar GC stationary phase that incorporates 5% phenyl substitution into the methylpolysiloxane backbone. This chemistry provides broad applicability, predictable retention behavior, and strong performance across volatile and semi-volatile analytes.
Low Bleed
The reduced release of stationary-phase material at elevated temperatures. Low bleed results in cleaner baselines, improved signal-to-noise, and higher mass spectral integrity—critical for GC/MS applications and trace-level detection.
Bonded and Cross-Linked
A durable column manufacturing approach in which the stationary phase is chemically bonded to the column walls and reinforced through cross-linking. This enables solvent rinsing, high-temperature operation, and long-term chromatographic stability.
USP Phase G27
A United States Pharmacopeia stationary-phase classification corresponding to 5% phenyl methylpolysiloxane selectivity. HP-5ms Ultra Inert aligns closely with this phase, making it suitable for regulated analytical workflows and validated methods.
Frequently Asked Questions â–¸
What advantages does the Ultra Inert (UI) version of HP-5ms offer?
HP-5ms Ultra Inert columns undergo Agilent’s most advanced deactivation process, minimizing active sites and significantly improving peak shapes for reactive analytes. This results in higher analyte recovery, lower detection limits, and improved sensitivity for GC/MS workflows.
How does HP-5ms UI differ from HP-5ms?
Both columns share the same 5% phenyl methylpolysiloxane selectivity, but the Ultra Inert version delivers much higher inertness and even lower bleed. HP-5ms UI provides cleaner baselines, improved mass spectral integrity, and better performance for acidic, basic, or adsorptive compounds.
What types of analyses benefit most from HP-5ms Ultra Inert columns?
HP-5ms UI is ideal for environmental semivolatiles, pesticides, forensic toxicology, pharmaceutical impurities, food and fragrance testing, and petrochemical profiling. Any GC/MS application requiring high sensitivity and low bleed benefits from UI performance.
Is HP-5ms Ultra Inert suitable for regulated or validated methods?
Yes. HP-5ms UI is considered equivalent to USP Phase G27, making it suitable for regulated workflows that require 5% phenyl methylpolysiloxane selectivity, while offering enhanced low-bleed and high-inertness performance.
Can HP-5ms UI columns be solvent-rinsed?
Yes. The bonded and cross-linked stationary phase is designed to withstand solvent rinsing, enabling removal of non-volatile residues and extending column lifetime in demanding workflows.