GKE-5006B - AdvanceBio N-Glycanase (PNGase F), >=2.5 U/mL
Agilent
$583.00
AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL. A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications). Supplied at a concentration of ≥ 2.5 U/mL. For a more concentrated formulation of ≥ 10 U/mL, which may be useful for native digestions, see GKE-5010B or GKE-5020B (EDTA-free).
- pH Range:
- 7.5-9.5
- Volume:
- 80 uL
- Enzyme Applications:
- Release of intact N-linked glycans from glycopeptides and glycoproteins;Structure and function studies of N-glycosylated glycoproteins;Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies
- Concentration:
- >= 2.5 U/mL
- Enzyme Formulation:
- 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5)
- Enzyme Source:
- Recombinant gene from Elizabethkingia meningoseptica, expressed in E
- coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. The enzyme is also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl- Beta -glucosaminyl)asparagine amidase. Enzyme Specific Activity:
- >= 10 units/mg
- Enzyme Specificity:
- Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless Alpha (1-3) core fucosylated, as in plant glycans and some insect glycans
- Asparagine must be peptide-bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free. Enzyme Unit Definition:
- One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 umole of denatured ribonuclease B per minute at 37 C, pH 7.5
- MolecularWeight:
- ~35,000 daltons
- pH Optimum:
- 8.6
- Units Of Product:
- 200 mU
