DB-225ms

DB-225ms column

DB-225ms Low Bleed GC/MS Columns

Agilent J&W DB-225ms columns are engineered for ultra-low bleed, high inertness, and reliable mid- to high-polarity separations—making them a highly effective choice for applications requiring precise resolution of structurally similar compounds. Built on a (50%-cyanopropylphenyl)-methylpolysiloxane stationary phase, DB-225ms provides significantly greater polarity than traditional 5% or 35% phenyl columns, enabling enhanced separation of challenging analytes and improved method selectivity.

DB-225ms columns are QC-tested under rigorous industry standards to ensure low bleed, accurate retention behavior, and reproducible results across laboratories and batches. Bonded, cross-linked, and solvent-rinsable, these columns offer durable performance even when exposed to demanding sample matrices or repeated temperature cycling. Their polarity profile makes them particularly well suited for resolving cis/trans isomers, especially fatty acid methyl esters (FAMEs), as required in food, nutritional, and lipid analysis workflows.

As a close equivalent to USP Phase G7, DB-225ms supports validated and regulatory methods while ensuring strong retention and selectivity for polar and semi-polar compounds. With an operating range from 40 to 240 °C, the column delivers strong performance in both isothermal and temperature-programmed methods, maintaining excellent peak shape, sensitivity, and mass spectral integrity in GC/MS workflows.

DB-225ms is widely used in applications such as FAME profiling, pesticide residue confirmation, flavor and fragrance analysis, food authenticity testing, environmental monitoring, and pharmaceutical impurity analysis—any workflow requiring enhanced polarity and precise separating power for isomeric or closely related analytes.

Key Definitions â–¸
(50%-Cyanopropylphenyl)-Methylpolysiloxane Phase
A high-polarity GC stationary phase consisting of 50% cyanopropylphenyl groups. This phase provides strong interactions with polar and semi-polar analytes and is ideal for separating positional, structural, and stereochemical isomers such as fatty acid methyl esters.
USP Phase G7
A United States Pharmacopeia classification for columns with a 50% cyanopropylphenyl methylpolysiloxane stationary phase. DB-225ms is equivalent to this phase, supporting validated and regulatory workflows in pharmaceutical, food, and environmental testing.
cis/trans Fatty Acid Methyl Esters (FAMEs)
Isomeric forms of fatty acids commonly profiled in nutritional analysis, food authenticity, and lipid research. DB-225ms provides strong selectivity for resolving cis and trans FAME isomers due to its mid- to high-polarity phase chemistry.
Mid- to High-Polarity Selectivity
A polarity range that provides stronger interactions with polar analytes than mid-polar phases such as DB-17ms. This enhanced polarity improves the separation of isomers and multifunctional compounds in food, environmental, and pharmaceutical workflows.
Solvent Rinsable
Indicates that the bonded and cross-linked stationary phase can be rinsed with appropriate solvents to remove non-volatile residues, helping extend column lifetime and maintain consistent performance in GC/MS analyses.
Frequently Asked Questions â–¸
What types of applications benefit most from DB-225ms columns?
DB-225ms is ideal for separating structurally similar or isomeric compounds such as cis/trans fatty acid methyl esters (FAMEs). It is commonly used in food authenticity testing, nutritional lipid profiling, pesticide residue analysis, flavor and fragrance analysis, and pharmaceutical impurity identification.
Why is DB-225ms preferred for FAME separations?
Its mid- to high-polarity (50% cyanopropylphenyl) stationary phase provides the necessary selectivity to resolve cis and trans isomers and closely related fatty acid methyl esters. This makes DB-225ms a preferred choice for nutritional and food authenticity testing, where precise isomer separation is essential.
Is DB-225ms suitable for GC/MS applications?
Yes. DB-225ms features ultra-low bleed performance, providing cleaner mass spectra and improved signal-to-noise ratios for sensitive GC/MS detection. Its inertness helps reduce adsorption of active compounds, supporting accurate quantitation at trace levels.
What is the operating temperature range for DB-225ms?
DB-225ms is operational from 40 Â°C to 240 Â°C. This temperature range makes it suitable for both isothermal and temperature-programmed methods used in food, environmental, and pharmaceutical analyses.
Is DB-225ms equivalent to any USP stationary phase?
Yes. DB-225ms is a close equivalent to USP Phase G7, which specifies a 50% cyanopropylphenyl methylpolysiloxane stationary phase. This ensures compatibility with validated and regulated methods requiring G7-equivalent separations.