DB-1301

Standard Polysiloxane GC Columns

Agilent J&W DB-1301 is a versatile GC column engineered with a (6% cyanopropyl-phenyl)-methylpolysiloxane stationary phase, providing low- to mid-polarity selectivity ideal for a wide range of environmental, industrial, and forensic applications. This balanced polarity profile enables improved separation of analytes that are difficult to resolve on nonpolar phases, including alcohols, aldehydes, solvents, and light semivolatiles.

DB-1301 is fully bonded, crosslinked, and solvent-rinsable, ensuring strong thermal stability, extended column lifetime, and consistent retention behavior across demanding sample matrices. The column is equivalent to USP phase G43, making it an ideal choice for compendial methods and regulated workflows requiring this specific selectivity.

Its robust design and polarity-enhanced chemistry make DB-1301 an excellent solution for laboratories analyzing complex mixtures such as VOCs, residual solvents, fragrances, and industrial chemicals. The column delivers reliable peak shapes, low bleed, and highly reproducible results, supporting both GC and GC/MS applications where accuracy and long-term stability are essential.

Key Definitions â–¸
(6% Cyanopropyl-Phenyl)-Methylpolysiloxane Phase
A stationary phase containing 6% cyanopropyl and phenyl functional groups blended into a methylpolysiloxane backbone. This formulation provides low- to mid-polarity selectivity, improving separation of analytes that exhibit polar or aromatic interactions.
USP Phase G43
A United States Pharmacopeia phase classification commonly used in environmental, industrial, and pharmaceutical applications. DB-1301 is equivalent to USP G43, enabling seamless method transfer and compliance with compendial procedures requiring this phase.
Low- to Mid-Polarity Column
A column designed to provide balanced interactions between analytes and the stationary phase, improving selectivity for compounds that are not well resolved on purely nonpolar phases. This polarity level supports mixtures containing alcohols, aldehydes, solvents, and light semivolatiles.
Bonded and Crosslinked
A chemically fortified stationary-phase design that improves thermal stability, reduces bleed, and allows solvent rinsing without damaging the column. This ensures longer lifetime and reproducible retention characteristics across repeated analyses.
Solvent Rinsable
Indicates that the column can be rinsed internally with organic solvents to remove contaminants without degrading or stripping the stationary phase—a critical advantage for high-throughput GC applications.
Frequently Asked Questions â–¸
What types of applications is DB-1301 best suited for?
DB-1301 is ideal for analyzing alcohols, aldehydes, ketones, residual solvents, VOCs, light semivolatiles, industrial chemicals, and aromatic compounds. Its balanced polarity provides improved separation of analytes that are not well resolved on purely nonpolar columns.
Is DB-1301 suitable for GC/MS workflows?
Yes. DB-1301 is bonded, crosslinked, and engineered for low bleed, making it fully compatible with GC/MS applications requiring clean baselines, stable retention times, and reproducible mass spectral integrity.
What does DB-1301’s USP phase G43 equivalence mean for method compliance?
Being equivalent to USP phase G43 means DB-1301 meets compendial requirements for methods that specify this stationary phase. Laboratories can confidently substitute DB-1301 in regulated workflows or legacy methods originally developed on G43-classified columns.
What advantages does the bonded and crosslinked design provide?
Bonding and crosslinking increase thermal stability, reduce stationary-phase bleed, and allow solvent rinsing without degrading the column. This increases uptime and overall column lifetime in workflows involving complex or dirty matrices.
How does DB-1301 compare to DB-624 or DB-5 style columns?
DB-1301 provides higher polarity than DB-5-type columns and lower polarity than DB-624, giving it a balanced selectivity profile. This makes it useful for applications that require stronger interactions than nonpolar phases provide, but not as much polarity as highly selective 624-type columns.