CP-Chirasil Val

CP-Chirasil Val column

Agilent CP-Chirasil Val GC Columns

Agilent J&W CP-Chirasil Val GC columns are engineered for high-precision separation of optically active compounds, with exceptional suitability for chiral amino acid analysis. Featuring a valine-based chiral stationary phase, these columns deliver strong enantioselective interactions that enable clear distinction between D- and L-amino acids as well as a wide variety of other chiral molecules encountered in food chemistry, pharmaceuticals, forensics, and biochemical research.

CP-Chirasil Val columns exhibit significantly lower bleed levels than many traditional chiral phases, supporting improved sensitivity and stable baselines even for trace-level quantitation. With a maximum operating temperature of 200 Â°C (both isothermal and programmed), the column provides the thermal robustness needed for complex sample matrices while maintaining high chiral selectivity and reproducibility.

Both antipodes of the Chirasil Val phase are available, enabling analysts to choose the optimal enantiomeric configuration for their application. On Chirasil-L-Val, D-amino acids elute before L-amino acids; on Chirasil-D-Val, the order is reversed. This predictable and customizable elution behavior is especially valuable when determining optical purity, as selecting the phase on which the minor enantiomer elutes first allows for the highest possible detection sensitivity and confidence in quantitation.
Key Definitions â–¸
CP-Chirasil Val
A valine-based chiral stationary phase used in GC for separating enantiomers—especially D- and L-amino acids. It offers strong enantioselective interactions and low bleed, supporting high-sensitivity analysis of chiral compounds.
Chiral Amino Acids
Amino acids that exist as D- and L- enantiomers—mirror-image forms that can differ in biological activity, flavor properties, or metabolic behavior. CP-Chirasil Val is widely used for determining their enantiomeric composition and purity.
L-Val and D-Val Phase Antipodes
Two forms of the Chirasil Val phase, each with opposite stereochemistry. On Chirasil-L-Val, D-amino acids elute first; on Chirasil-D-Val, L-amino acids elute first—allowing analysts to choose the configuration that delivers the best detection of minor enantiomers.
Optical Purity Determination
The process of quantifying the relative proportions of two enantiomers in a sample. Using the Chirasil Val antipode that elutes the minor enantiomer first improves sensitivity, enabling more accurate assessment of low-level enantiomeric impurities.
Frequently Asked Questions â–¸
What makes CP-Chirasil Val effective for amino acid enantiomer separations?
Its valine-derived chiral stationary phase provides strong stereoselective interactions, enabling reliable resolution of D- and L-amino acids. This makes it ideal for applications requiring high-sensitivity determination of optical purity.
Why are both L-Val and D-Val versions of the phase useful?
Each antipode reverses the elution order of D- and L-amino acids. Selecting the version that elutes the minor enantiomer first enables lower detection limits, improving the accuracy of optical purity testing.
What is the maximum operating temperature of CP-Chirasil Val columns?
CP-Chirasil Val columns offer a Tmax of 200 °C, both isothermally and in programmed runs, providing adequate thermal range for chiral amino acid and related compound analysis while maintaining phase stability and low bleed.
Why are CP-Chirasil Val columns preferred for optical purity determination?
Their predictable enantiomer-elution behavior allows analysts to choose the phase configuration that resolves the minor component first. This enhances sensitivity for trace-level enantiomers and provides more accurate purity assessments in complex samples.