Column Heaters
Column Heater
Control Hot Sleeve
HPLC Column Ovens and Sleeves
In high-performance liquid chromatography (HPLC), temperature control is critical for maintaining retention time consistency, selectivity, and peak symmetry. Even small temperature variations—whether seasonal or daily—can significantly impact chromatographic performance.
To stabilize column temperature, laboratories use HPLC column ovens that provide uniform heating across the column. When operating substantially above ambient conditions, a pre-column hot sleeve helps ensure the mobile phase reaches the column at the proper temperature, preventing shifts in analyte behavior and improving reproducibility.
Chrom Tech offers both the H-105 Column Heater for precise temperature control and the Hot Sleeve for solvent preheating—essential tools for achieving stable, repeatable HPLC separations.
Frequently Asked Questions
Why is temperature control important in HPLC?
Temperature affects analyte retention, viscosity, and separation efficiency. Stable temperature ensures consistent retention times, sharper peaks, and better reproducibility in HPLC analysis, especially important for high throughput labs. In many applications, increasing the column temperature can reduce system back pressure.
When should I use a pre-column heat exchanger?
A pre-column heat exchanger should be used when heating solvents significantly above ambient temperature. Pre-Column Heat Exchangers are necessary to prevent a temperature gradient on the HPLC column, ensuring the solvent temperature is at the same temperature as the HPLC column.
What temperature range can the H-105 column heater maintain?
The H-105 Column Heater offers precise temperature control across typical HPLC operating ranges, maintaining uniform heat distribution for reliable and repeatable results.
