Captiva ND Lipids

Agilent Captiva ND Lipids Sample Preparation

Agilent Captiva ND Lipids devices combine the automation-friendly design of Captiva ND with a specialized chemical filtration layer to deliver enhanced lipid and protein removal for bioanalytical workflows. This integrated approach improves quantitative accuracy by reducing ion-suppressing phospholipids and matrix components that commonly interfere with LC-MS analysis.

The system pairs Captiva ND’s superior flow characteristics with a unique sorbent chemistry, producing cleaner extracts while maintaining consistent analyte recovery. When used with methanol, Captiva ND Lipids provides more effective lipid removal than traditional acetonitrile-based precipitation, supporting more precise and reproducible quantitation.

Key Benefits

  • Improves quantitative reproducibility by removing ion-suppressing phospholipids and proteins
  • Combines Captiva ND ease of use with a proprietary chemical filter for enhanced cleanup
  • Delivers exceptional flow performance for faster sample processing
  • Optimized for methanol-based precipitation to maximize lipid removal efficiency
  • Produces cleaner, MS-ready extracts for high-confidence LC-MS workflows

Captiva ND Lipids is an effective replacement for conventional acetonitrile precipitation methods, offering superior lipid removal and improved method robustness when methanol is used as the precipitation solvent. Chrom Tech supplies Captiva ND Lipids as part of a complete Agilent sample preparation portfolio to support consistent, high-quality analytical results.

PART NO DESCRIPTION QTY
CAPTIVA ND LIPIDS
A59640002SK Captiva ND Lipids 96-well filtration starter kit (includes CaptiVac vacuum collar, 2 ND Lipids filter plates, 2 Captiva 96 deep-well 1 mL collection plates, 2 pierceable covers) ea
A59640002RK Captiva ND Lipids 96-well filtration replacement kit (includes 2 ND Lipids filter plates, 2 Captiva 96 deep-well 1 mL collection plates, 2 pierceable covers) ea
A59640002B Captiva ND Lipids 96-well filtration plate 100/pk
A59640002I Captiva ND Lipids 96-well filter plate, 1 mL well ea
A59640002V Captiva ND Lipids 96-well filter plates, 1 mL well 5/pk
A8961008 DuoSeal 96-well plate seals 10/pk
Key Definitions â–¶
Captiva ND Lipids
A sample preparation device that combines Captiva ND flow control with a specialized chemical filtration layer to selectively remove lipids and proteins for cleaner LC-MS extracts.
Phospholipids
Lipid-based matrix components commonly responsible for ion suppression in mass spectrometry, making their removal critical for accurate quantitation.
Chemical Filtration Layer
A proprietary sorbent phase integrated into Captiva ND Lipids that selectively binds lipid species while allowing target analytes to pass through.
Methanol Precipitation
A protein precipitation technique using methanol as the solvent, shown to enhance lipid removal performance when paired with Captiva ND Lipids compared to acetonitrile.
Ion Suppression
Reduced analyte signal in LC-MS caused by co-eluting matrix components such as lipids, negatively impacting sensitivity and quantitative accuracy.
Frequently Asked Questions â–¶
What is Captiva ND Lipids used for?
Captiva ND Lipids is used for efficient removal of phospholipids and proteins during sample preparation, producing cleaner extracts and improving quantitative accuracy in LC-MS workflows.
How does Captiva ND Lipids differ from standard Captiva ND?
Captiva ND Lipids incorporates a specialized chemical filtration layer in addition to the Captiva ND flow-control design, enabling selective lipid removal while maintaining analyte recovery.
Which solvent works best with Captiva ND Lipids?
Methanol is recommended for optimal lipid removal, and has been shown to outperform acetonitrile when used with Captiva ND Lipids.
Does Captiva ND Lipids affect analyte recovery?
No. The system is designed to selectively remove lipid components while preserving target analytes, supporting reliable quantitative results.
Is Captiva ND Lipids compatible with automation?
Yes. Captiva ND Lipids maintains the non-drip, high-flow design of Captiva ND, making it compatible with vacuum manifolds and automated liquid handling platforms.