Lipid Extraction SPE

Agilent Bond Elut Lipid Extraction SPE

Agilent Bond Elut Lipid Extraction SPE cartridges and 96-well plates utilize innovative Enhanced Matrix Removal–Lipid (EMR-Lipid) sorbent technology, combining size exclusion and hydrophobic interactions to selectively trap and isolate lipids. This advanced approach provides highly efficient lipid extraction for lipidomics, profiling, and biochemical analysis, resulting in cleaner samples and improved data quality.

Effective lipid analysis requires removing co-extractives and non-lipid impurities that interfere with detection. Bond Elut Lipid Extraction SPE formats offer a more reproducible, efficient, and automatable workflow compared to traditional liquid-liquid extraction (LLE) methods—significantly improving consistency across replicates and reducing sample preparation time.

Key Features

  • Streamlined and reproducible lipid extraction for analytical and omics workflows
  • Integrates Agilent’s EMR-Lipid sorbent technology for precise lipid isolation
  • Delivers significant time savings versus conventional LLE techniques
  • Reduces sample-to-sample variability and enhances statistical confidence
  • 96-well plate format supports automation and high-throughput sample processing

The EMR-Lipid sorbent’s selective retention mechanism effectively isolates lipid components while removing phospholipids, proteins, and other matrix interferences. This ensures cleaner chromatograms, higher recovery rates, and consistent quantitation— ideal for high-resolution LC/MS or GC/MS lipid analysis.

General Instructions

  1. Crashing solvent: Use acetonitrile (ACN) with 5% methanol (MeOH). The small MeOH fraction produces finer protein precipitates, simplifying homogenate transfer.
  2. Washing solvent: A mixture of ACN with 10–20% water ensures effective removal of non-lipid impurities prior to elution.
  3. Elution solvent: Use MeOH with dichloromethane (DCM), chloroform, or 1-chlorobutane. A minimum of 50% MeOH is essential for lipid release. Agilent recommends DCM/MeOH (1:2, v/v) or chloroform/MeOH (1:1, v/v) for optimal recovery.

Frequently Asked Questions

What makes EMR-Lipid technology different from traditional lipid extraction methods?

EMR-Lipid combines size exclusion and hydrophobic interaction mechanisms to specifically retain lipids, reducing matrix interferences and eliminating the need for time-consuming LLE steps.

Can Bond Elut Lipid Extraction SPE be used with automated platforms?

Yes. The 2 mL 96-well plate format is designed for automation, allowing integration with robotic liquid-handling systems for high-throughput lipidomics workflows.

What solvents are recommended for optimal lipid recovery?

Agilent recommends DCM/MeOH (1:2, v/v) or chloroform/MeOH (1:1, v/v) as elution solvents to achieve complete lipid recovery and ensure compatibility with downstream analysis.