Liquid Chromatography Columns and Supplies

By Chrom Tech – Your Chromatography Consumables Partner Since 1983

Overview

Chromatography columns are the cornerstone of separation science, allowing scientists to isolate and analyze individual components within complex mixtures. Precision separations are essential for research, production, and quality control across pharmaceuticals, environmental testing, food safety, and chemical manufacturing.

At Chrom Tech, we supply a full range of liquid chromatography (LC) and gas chromatography (GC) columns from leading brands such as Agilent, Restek, and ThermoFisher. Our selection delivers the accuracy, repeatability, and performance your lab depends on.

Understanding Chromatography Columns

Chromatography columns function by separating compounds according to their chemical and physical interactions within the column. Differences in polarity, size, and affinity determine each compound’s movement, resulting in a clear, quantitative separation.

• Exceptional accuracy and reproducibility
• Adaptable to a wide variety of sample types
• Available for analytical, preparative, and high-throughput workflows

Chromatography Techniques and Column Types

Each chromatography technique employs unique mobile and stationary phases to target specific compounds. The two most widely used methods are:

• Liquid Chromatography (LC): Uses a liquid mobile phase, ideal for separating polar and thermally unstable compounds.
• Gas Chromatography (GC): Uses a gaseous mobile phase for volatile and semi-volatile analytes.

Chrom Tech provides an extensive selection of both LC and GC columns to match your analytical requirements with proven reliability and performance.

Liquid Chromatography Columns: HPLC and UPLC

High-Performance Liquid Chromatography (HPLC) columns remain the gold standard for reproducible separations. For faster analyses and enhanced efficiency, Ultra-Performance Liquid Chromatography (UPLC) columns deliver superior resolution at higher pressures. Chrom Tech supplies columns engineered for durability, consistent flow, and solvent compatibility—essential for routine and advanced LC applications.

• Optimized for aqueous and organic mobile phases
• Available in multiple particle sizes and dimensions
• Compatible with a wide range of detectors and instrumentation

Gas Chromatography Columns

GC columns are designed to handle volatile samples requiring thermal stability and inertness. Selecting the correct stationary phase, column diameter, and temperature range ensures optimal efficiency and peak shape.

• Wide selection of non-polar, polar, and specialty stationary phases
• High temperature tolerance and reproducibility
• Available in fused silica and metal formats for diverse applications

Explore more: Gas Chromatography Columns and Supplies

Leading Column Brands

• Agilent HPLC Columns – Renowned for precision, consistency, and longevity.
• Restek HPLC Columns – Known for innovation, including unique BiPhenyl Phase selectivity.
• ThermoFisher HPLC Columns – Offer superior compatibility across a wide range of analytical applications.

Performance Factors Affecting Separation

• Particle Size: Smaller particles improve surface area, enhancing separation efficiency and resolution.
• Column Length: Longer columns increase resolving power for complex samples.
• Temperature Control: Proper temperature management ensures stable retention times and reproducible results.

Applications Across Industries

• Pharmaceuticals: Drug purity testing, impurity profiling, and stability analysis.
• Environmental Testing: Monitoring organic pollutants, pesticides, and trace contaminants.
• Food Safety: Detecting additives, preservatives, and nutritional markers.
• Chemical Manufacturing: Quality assurance and raw material verification.

Column Care and Maintenance

• Flush columns regularly to prevent buildup and carryover.
• Store under recommended solvent and temperature conditions.
• Follow manufacturer protocols to extend column life and maintain consistency.

Conclusion

Chromatography continues to evolve with new materials, smaller particles, and enhanced performance characteristics. Selecting high-quality columns and maintaining them properly is the key to achieving reproducible, high-resolution separations. Partner with Chrom Tech for industry-leading liquid chromatography columns and supplies that keep your analyses accurate, efficient, and dependable.