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Posted by Chrom Tech on 14th Oct 2025

Should I Use a Guard Column

In any liquid chromatography (LC) system, the HPLC column is the heart of the separation process. It is here that analytes are isolated for both qualitative and quantitative analysis. While HPLC colu …

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Posted by Chrom Tech on 14th Oct 2025

Are Hydrogen Generators Safe?

Laboratories traditionally rely on compressed hydrogen cylinders for their gas supply, but this approach carries safety concerns and can cause unexpected downtime when deliveries are delayed. Switch …

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Posted by Chrom Tech on 14th Oct 2025

HPLC Pump Care and Troubleshooting

Routine care and preventative maintenance are key to reliable performance from your Chrom Tech continuous process pumps. Below are common support topics, troubleshooting steps, and maintenance prac …

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Posted by Chrom Tech on 14th Oct 2025

How To Choose a Syringe Filter

Protect your HPLC system and achieve consistent results with Chrom Tech’s syringe filters. Designed for quick, single-use filtration, these affordable filters efficiently remove particulates f …

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Posted by Chrom Tech on 14th Oct 2025

Acetonitrile vs. Methanol for Reverse Phase Chromatography

Acetonitrile vs. Methanol in Reversed-Phase Chromatography Acetonitrile (ACN) and Methanol (MeOH) are the two most common organic modifiers used in reversed-phase HPLC. Each solvent exhibits unique …

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Posted by Chrom Tech on 14th Oct 2025

Pore Size vs. Particle Size in HPLC Columns

Selecting the right HPLC column is crucial for achieving precise, reproducible chromatographic results. Chemical factors such as stationary phase type and pore size influence selectivity and retent …

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Posted by Chrom Tech on 14th Oct 2025

The Ultimate Guide to Chromatography Paper

Key Highlights Chromatography paper is a specialized medium used for separating mixtures into their individual components. Made from high-purity cellulose fibers, it provides consistent, reproduci …

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Posted by Chrom Tech on 14th Oct 2025

Ultimate Guide to Protein Purification Methods

Key Highlights Comprehensive overview of protein purification methods, from extraction to advanced chromatographic and electrophoretic techniques. Explores the importance of chromatography, electr …

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Posted by Chrom Tech on 14th Oct 2025

Understanding Chromatography Paper: Uses and Methods

Key Highlights Chromatography paper separates mixtures using differences in molecular affinity for the stationary and mobile phases. Made from cellulose fibres, it combines absorbency and capillar …

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Posted by Chrom Tech on 13th Oct 2025

Agilent HPLC Supplies: Maximize Your Lab Budget

Managing a lab budget while maintaining high scientific standards can be challenging. Optimizing the use of Agilent HPLC supplies like capillary tubing, pump seals, frits, and valves ensures efficie …

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21st Nov 2024

Effective HPLC Solvent Containment Solutions

Key HighlightsEffective HPLC solvent containment solutions are crucial for ensuring safety in the laboratory.Understanding the risks associated with HPLC solvents is essential for implementing proper …

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21st Nov 2024

Exploring the Diverse Applications of HPLC

Key HighlightsHPLC stands as a cornerstone analytical technique across various scientific disciplines, enabling the separation, identification, and quantification of compounds within mixtures.Its appl …

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20th Nov 2024

Mastering Size Exclusion Chromatography Techniques

Key HighlightsSize exclusion chromatography (SEC), also known as gel filtration chromatography, separates molecules based on their hydrodynamic size.This technique is vital in various scientific field …

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20th Nov 2024

Mastering the Basics of Chromatograms

Key HighlightsThis blog explores the fundamentals of chromatograms, key components, and their significance in various scientific fields.We break down different types of chromatography, such as gas and …

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20th Nov 2024

Mastering TLC Chromatography: A Comprehensive Guide

Key HighlightsTLC chromatography is a versatile technique for separating and analyzing mixtures.It relies on the principle of differential migration of compounds based on their affinities for a statio …

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20th Nov 2024

Understanding and Analyzing Chromatogram Data

Key HighlightsA chromatogram visually represents the separation of a mixture's components through chromatography.Analyzing chromatogram data involves inspecting the baseline, peak shapes, and retentio …

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20th Nov 2024

Understanding Chromatography: A Simple Guide

Key HighlightsChromatography is a vital technique in analytical chemistry for separating and analyzing complex mixtures.It involves the use of a mobile phase and a stationary phase to separate differe …

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20th Nov 2024

Chromatogram Explained: A Comprehensive Guide

Key HighlightsChromatography is a versatile technique used to separate, identify, and purify components within a mixture.The process relies on the differential interactions of analytes with a stationa …

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20th Nov 2024

Mastering Chromatogram Analysis: A Comprehensive Guide

Key HighlightsThis comprehensive guide explores chromatogram analysis, covering its principles, types, and applications.Learn about the critical role of stationary and mobile phases in the separation …

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20th Nov 2024

Understanding Liquid Chromatography: Key Principles and Techniques

Key HighlightsLiquid chromatography is a versatile analytical technique used to separate, identify, and quantify components within a mixture.It relies on the differential interactions of sample compon …

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20th Nov 2024

Mastering Chromatographic Analysis: Techniques and Insights

Key HighlightsChromatography stands as a cornerstone technique in analytical chemistry, enabling scientists to separate, identify, and quantify individual components within complex mixtures.Its versat …

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20th Nov 2024

Mastering Protein Separation with Ion Exchange Chromatography

Key HighlightsHighly Selective: Ion exchange chromatography excels in separating proteins with subtle charge differences.Versatile Applications: It's widely used in various fields, from purifying phar …

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20th Nov 2024

Mastering Chromatography Results: A Comprehensive Guide

Key HighlightsChromatography is a crucial technique used to separate and quantify mixtures of organic compounds in analytical laboratories.Understanding the principles of chromatography, including the …

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20th Nov 2024

Understanding Gel Permeation Chromatography Basics

Key HighlightsGel permeation chromatography (GPC) is a powerful analytical technique used to determine the size and distribution of molecules, particularly polymers.This method separates molecules bas …

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20th Nov 2024

Understanding Chromatography: Basics and Applications

Key HighlightsChromatography is a versatile technique for separating mixtures into their individual components.This method relies on the differential affinities of analytes towards a stationary phase …

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20th Nov 2024

Mastering Chromatogram Analysis: A Step-by-Step Guide

Key HighlightsThis guide provides a comprehensive overview of chromatogram analysis, from understanding the basics to practical tips for beginners.Learn how to interpret chromatograms, identify peaks, …

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20th Nov 2024

Understanding Adsorption Chromatography: Principles and Uses

Key HighlightsAdsorption chromatography is a versatile separation technique used to isolate and purify different components of a mixture.This method relies on the differential adsorption of solute mol …

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20th Nov 2024

Mastering Chromatogram Interpretation: A Step-by-Step Guide

Key HighlightsMaster the art of interpreting chromatograms with a detailed step-by-step guide.Understand the fundamentals of chromatography and its crucial role in analytical science.Learn to prepare …

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20th Nov 2024

Understanding Chiral Chromatography: A Comprehensive Guide

Key HighlightsChiral chromatography plays a crucial role in separating enantiomers for various industries, especially in pharmaceutical research and drug development.Understanding molecular chirality …

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20th Nov 2024

Exploring the Total Ion Chromatogram (TIC) in Mass Spectrometry

Key Highlights The Total Ion Chromatogram (TIC) is a fundamental tool in analytical chemistry, providing a visual representation of ion abundance over time in mass spectrometry. TIC is generated by …

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20th Nov 2024

Mastering Chromatography for Pure Results

Key HighlightsChromatography stands as a cornerstone technique in analytical chemistry, facilitating the separation, identification, and purification of components within a mixture.The technique hinge …

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20th Nov 2024

Mastering Column Chromatography: Techniques and Tips

Key Highlights Column chromatography is a fundamental technique allowing chemists to isolate and purify individual compounds from complex mixtures. Choosing suitable stationary and mobile phases is …

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20th Nov 2024

Exploring the Evolution of Liquid Chromatography Mass Spectrometry

Key HighlightsLiquid chromatography mass spectrometry (LC-MS) is an indispensable analytical technique that combines the separation power of liquid chromatography with the detection capabilities of ma …

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20th Nov 2024

Flash Chromatography Explained: A Comprehensive Guide

Key Highlights Flash chromatography is a purification technique used to separate mixtures of compounds. It is a faster and more efficient method compared to traditional column chromatography. This t …

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20th Nov 2024

Understanding Chromatography Diagrams: Principles and Types

Key Highlights Chromatography is a versatile analytical technique used to separate, identify, and purify components of a mixture. It relies on the differential interaction of components with a stati …

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20th Nov 2024

Understanding the Differences: Chromatogram vs Chromatograph

Key Highlights Chromatography is a widely used analytical technique employed to separate, identify, and purify the individual components of a complex mixture. A chromatogram is the visual output of …

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20th Nov 2024

Understanding Gel Permeation Chromatography Techniques

Key HighlightsGel permeation chromatography (GPC) is a powerful technique used to separate molecules based on their size.It is widely employed in various fields like polymer science, biotechnology, an …

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20th Nov 2024

Understanding Mass Chromatograms: Key Concepts Explained

Key HighlightsMass chromatograms play a vital role in modern chemistry, aiding in the analysis of complex mixtures and providing valuable insights into the molecular composition of samples.Different m …

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20th Nov 2024

Understanding Chromatogram Sanger Sequencing Analysis

Key Highlights Chromatogram Sanger sequencing analysis is essential for validating DNA sequencing results and ensuring accuracy. Understanding peak patterns, identifying artifacts, and troubleshooti …

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20th Nov 2024

Comprehensive Guide to Chromatographic Solutions

Key HighlightsThis guide explores the power of chromatographic solutions in analytical science, focusing on leading techniques like HPLC and GC.We examine the core principles of chromatography, its di …

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20th Nov 2024

Chromatogram vs Chromatograph: Key Differences Explained

Key Highlights Chromatography is a versatile separation science technique, used to separate, identify, and purify components of a mixture. A chromatograph is the instrument used in chromatography, w …

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20th Nov 2024

Understanding Chromatography Graphs for Beginners

Key Highlights Chromatography graphs are visual representations of the separation process in chromatography. Peaks on the graph represent different compounds in a sample, with their size and positio …

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20th Nov 2024

Journey Through the History of Paper Chromatography

Key HighlightsPaper chromatography is a cornerstone technique in analytical chemistry, boasting a rich history marked by continuous innovation and refinement.From its humble beginnings as a method for …

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19th Nov 2024

Ultimate Guide to Chromatography Cartridges

Key HighlightsChromatography cartridges offer a versatile and efficient method for purifying compounds in various scientific disciplines.Understanding the different types of cartridges, their componen …

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19th Nov 2024

Adsorption Chromatography: Past to Present

Key Highlights Adsorption chromatography stands as a cornerstone technique in analytical chemistry, playing a crucial role in separating and analyzing complex mixtures. This method hinges on the pri …

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19th Nov 2024

Top Chromatography Strips for Accurate Results

Key HighlightsChromatography strips are essential for separating mixtures into individual components.Accurate results depend on choosing high-quality strips with suitable material and absorption rates …

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19th Nov 2024

Ultimate Guide to Chromatography Paper Sheets

Key HighlightsDiscover the essential role of chromatography paper in chemical separations.Learn about the types and features of chromatography paper sheets available.Understand how to select the right …

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19th Nov 2024

Disposable Chromatography Columns for Bioseparation that are Simple to Utilize

Key HighlightsDisposable chromatography columns offer a convenient and efficient solution for bioseparation processes.These columns are pre-packed with various chromatographic media, simplifying the p …

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