If you'd like to learn how HPLC pumps were used in the production of Lipid-based Nanoparticles for delivering mRNA-based vaccines, our experts explain here.
Work in a chromatography lab? Make sure you understand what endcapping is in HPLC columns. Our experts break down everything you need to know here.
Anyone who works in a lab should know HPLC pump care and troubleshooting to ensure peak performance. You can learn more in our comprehensive guide here.
If you work in a laboratory, you should know how to regenerate a C18 HPLC column. The experts at Chrom Tech have you covered in our detailed guide here.
On April 22nd, people across the globe will initiate sustainability efforts, connect with nature, and educate others on how we can reduce our carbon footprint in celebration of the 51st annual Earth Day. This year, the theme is “Restore Our Earth”, and we would like to hear what others are doing to participate. Chrom Tech founder, Steve Pierson sought after ways to reduce waste, re-use materials, and utilize recyclable products wherever possible at the company, many of which are still everyday practices today.
In general, modern chromatography liquid autosamplers accept 12 x 32mm vials, so we will assume that a 12 x 32mm vial is compatible with your autosampler (Headspace autosamplers accept 20mm wide autosampler vials). Here, we have outlined the simple steps in selecting the best chromatography vial for your analysis.
Superficially porous particles (SPPs) have a solid core surrounded by a thin, porous shell rather than porous throughout the entire particle, as is the case in traditional, fully porous particles. The solid core limits diffusion distance and improves separation speed. Superficially porous particles also have a narrow particle size distribution, improving efficiency and resolution. Another advantage of superficially porous particles is they can be used on both HPLCs and UHPLCs. Because of the variety of phases now available and the compatibility with both new and older HPLC systems, superficially porous columns are many chromatographers’ first choice when developing a new HPLC method.
Hydrogen is increasing in popularity in the GC world as a carrier gas. In this blog, will explore why more and more laboratories are turning to hydrogen.
In order to understand which pumps are used in HPLC, we will first explore why high pressure is needed in HPLC.
It is recommended to change your HPLC pump’s piston seals during planned preventative maintenance. With regular pump usage, this is recommended every six months. And, remember, if you have a dual-piston pump, you have two piston seals to replace.
Did you know?! 100% of our salesforce has work experience in a chromatography laboratory. Plus, our customer service works hard to ensure on-time delivery. 98% of our orders ship on time – we work to get your supplies to you by the estimated delivery date. Our loyal customers receive their complete orders within 2 days, on average.
HPLC Column temperature affects retention time, selectivity, peak shape, and pressure. Let’s look at this in more detail.
Inductively Coupled Plasma - Mass Spectrometer; that is a mouthful. ICP-MS for short is a more recent advancement in analytical instrumentation designed to detect low and trace levels of metals in various samples in their native form or as part of a compound. These samples can be derived from everyday things our families encounter.
If you're looking to understand how a pulse dampener works in an HPLC pump, the experts at Chrom Tech have you covered. Learn more in our detailed guide here.
Proteins make up every living organism and as you can imagine, many industries (pharmaceutical, clinical, environmental, etc.) rely on understanding them. One means of analyzing a protein is through LC-MS. A difficult question that researchers often get hung up on is “What LC column should I use?”.. The answer is: it depends. It depends on the type of experiment you are going to be performing, the type of protein you are working with, and other factors. Here we will break down the ideas behind this question through the topics below.
PEEK tubing has its own strengths over stainless steel tubing in HPLC, so when should you use PEEK tubing? Our experts discuss everything you need to know here.
If you're wondering if there is a universal membrane for syringe filters, we've got you covered. Learn the answer and more in our comprehensive article here.
All lab professionals must know the types of fittings that are used in HPLC. The experts at Chrom Tech cover everything you need to know in this detailed guide.
Laboratories should follow the EPA's recommendations when it comes to washing HPLC vials. You can learn the EPA's guidelines in our comprehensive guide here.
All laboratory professionals should understand particle and pore size in HPLC columns. Read our comprehensive guide here to learn from our experts.
Have you always wondered what 96 well microplates are used for? We've got you covered. The experts at Chrom Tech break down everything you need to know here.
Although hydrogen gas cylinders are commonly found in the laboratory, they present a variety of safety risks and unexpected instrument downtimes due to inconsistent delivery schedules. These issues can be eliminated by switching to an on-site gas generator. Read more to discover the answers to frequently asked questions concerning the safety of Hydrogen generators.
Lab professionals must understand the difference between a union and an adapter in HPLC. Read our guide to learn what makes unions and adapters different.
Every lab professional must understand HPLC column care and maintenance. Our experts at Chrom Tech cover just that in our guide; read here to learn more.
If your lab uses gas chromatography, learn why you should use GC derivatization reagents. The experts at Chrom Tech break down everything you need to know here.
If you're looking to learn how to streamline your sample preparation workflow, we have you covered. Read here to learn from the experts at Chrom Tech.
Choose Chrom Tech economical HPLC syringe filters to remove particulates from your liquid samples prior to injection on your HPLC.
Are you wondering how long your column will last and how many injections you can get on an HPLC column? Chrom Tech's professionals will help you in our detailed guide here.
HPLC pumps or solvent delivery pumps can be used for many applications, not just specific to chromatography applications.
Liquid liquid extraction (LLE) is a labor-intensive method in which you are separating compounds into two immiscible liquids: the aqueous layer and an organic solvent layer.
By definition, pressure is inherent in HPLC systems because solvent is being pumped through a packed bed of small particles.
Sample preparation is an important step in chromatography, maximizing system uptime and extending column lifetime. Here are a few fundamental sample preparation processes.
Acetonitrile (ACN) and methanol (MeOH) are the most common organic modifiers in reversed-phase chromatography. Both solvents offer certain advantages and disadvantages. This guide takes a deeper look at acetonitrile vs. methanol for reverse phase chromatography.
Outgassing and bubble formation can negatively impact the flow of the mobile phase and the analysis of analytes. Degassing is used to prevent these issues.
Analytical columns can fail over time due to particulates, solvent impurities, and sample contents. Filters and guard columns protect the column from damage.
Is buying pre-cut stainless steel tubing worth the premium? In this blog, we discuss the benefits of pre-cut vs. cutting your own length of tubing.
One of the biggest concerns in our world today is the state of the environment. Every day, our planet faces new challenges contributing to climate change, deforestation, and ozone depletion.
Many factors play a role in deciding which vial is best for your needs such as sample volatility, sample amount, and light sensitivity. Choosing the right vial is imperative to increasing ROI and operability and decreasing sample degradation due to improper collection or storage. Follow this guide for tips on how to select chromatography vials for the best results...
Liquid Chromatography can be achieved through many methods including reverse-phase and hydrophilic interaction liquid chromatography (HILIC). Liquid chromatography is a form of column chromatography that uses an HPLC pump to pump an analyte in a solvent (the mobile phase) at high pressure through a column (stationary phase) to separate...
Vials and 96-well plates are standard consumables used in chromatography. While vials were previously the industry standard, well plates have become more popular in recent decades. Both vials and well plates still hold advantages that make them viable options in chromatography processes. Vials vs. 96-well plates for chromatography is a debate worth investigating before deciding which to use in your lab.
Chromatography is a vital tool used in many industries to determine the composition of a mixture. Chromatography is widely known for its role in scientific research, although it holds importance in other industries. Chromatography plays a vital role in industries we interact with quite often. Pharmaceuticals, clinical trials, environmental and chemical safety, food and beverage, drug testing, forensics, petroleum creation, and molecular biology are some of the most common uses of chromatography.
The COVID-19 pandemic has created unprecedented struggles across the world due to symptom variability and high spread rate. As COVID-19 continues to surge through the U.S., there is an increasing need for technology that will help us understand viruses like COVID-19 faster.
Reliable and accurate results for pesticide and mycotoxin testing is of utmost importance for the safety of people consuming cannabis-derived products such as cannabis brownies and other edibles. Edibles are one of the most popular categories of consumption of cannabis-based products, and brownies are one of the most commonly consumed edible. Brownies contain high levels of potential interferences (carbohydrates and fats), so methods developed for this application will be a great starting point for similar matrices.
As many laboratories continue to experience drastic budget cuts, lab managers are tasked with difficult decisions on how they can spend less money without sacrificing throughput and accuracy of results. Chrom Tech offers high quality chromatography vials at an affordable price. As touched on in a previous blog article, Chrom Tech is now offering limited volume inserts pre-loaded into autosampler vials to reduce labor time and costs for your laboratory while maintaining fair and competitive prices on the vials.
The COVID-19 pandemic has affected laboratories in many ways, including many cutting the number of employees in the laboratory at any given time. Spreading the virus is a main concern across workplaces, and eliminating unnecessary touch points between employees is more important now than it has ever been. An inhouse stand alone gas generator reduces the number of personnel contact involved with the use of gas bottles and cylinders in the laboratory. Once a generator is installed in the laboratory, minimum user interaction is required for 24/7 usage, and minimal maintenance is required.
The coronavirus pandemic continues to cause shortages on hand sanitizers as people are doing their part to prevent the spread of COVID-19. Hand sanitizers are hard for consumers to find, but some companies with laboratories equipped for laboratory testing are stepping up to help meet the demands by using their isopropanol and ethanol to make sanitizer.
The current economic situation revolving around the COVID-19 pandemic will continue to create lasting effects on our economy. Chrom Tech is working to support our customers throughout and beyond the COVID-19 situation. We continue to promote cost and time savings in the laboratory, while also adding to our chromatography portfolio for those who are on the front end of this viral pathogen research.
If you’re looking for Immunoassay Plates for ELISA in your COVID-19 research, we have them available in 96-well and 384-well formats. Our plates are made of clear polystyrene with flat bottoms. The standard footprint of the plates allows for automation to increase your laboratory’s throughput.
A message from the president: Chrom Tech has solutions to reduce your costs and increase efficiency in the lab. Laboratories experiencing these pressures is nothing new, and we’ve been on the front end of improving laboratory efficiency for years.
Eliminate unnecessary safety risks, and avoid citations for improper waste containment by utilizing our waste containment system kit. A properly plumbed HPLC system, from inlet, to outlet, to waste is a crucial aspect in ensuring the safety of your laboratory’s employees.
Laboratories across the world are responsible for quickly and accurately reporting research results. According to the CDC, 70% of medical decisions made depend on test results from a laboratory. An inefficient laboratory can cause unnecessary expenses, but also delay research and results and even have detrimental effects on patients.
Whether analyzing for drugs, forensic evidence, or specific compounds in a specific clinical matrix, accurate sample analysis is important. For example, as the American Chemical Society discusses, “The credibility of the legal system critically depends on forensic tests that are consistent, accurate, and scientifically valid.” If a sample is analyzed inaccurately, serious consequences may occur so it is important that precautions are taken to eliminate margins of error in every step of the analysis.
As the Cannabis industry grows in the United States, laboratories are required to follow challenging sample preparation and analysis procedures to accurately measure active compounds, according to Cannabis Science and Technology. The accuracy of the sample preparation phase is extremely important, and variability between analysts can be very problematic for laboratories with a manual dilution process. Using the Microlab 600 can reduce the %RSD by more than 50% when compared to hand pipetting.
Chrom Tech is constantly working to make shopping easier for consumers. Replacing the various capillary tubing connections in your HPLC can be intimidating and confusing. In our latest efforts, we put together a capillary replacement guide for your standard Agilent HPLC systems.
As women and men age, many face issues that can be treated by hormone replacement therapy, according to the Women’s Healthcare Associates. However, hormone steroids are metabolized differently from person to person, which leads to complexity in treating a patient effectively without increasing a person’s risk for diseases such as cancer, dementia, and loss of bone density. It is important for researchers to be able to quantitatively determine endogenous steroid levels in order to monitor patient health and success with the hormone replacement therapies.
Cannabis products have been marketed as a natural cure-all, leading way to CBD oils, creams, pills, and even gummies that can be found all over stores and marketplaces in America today. If you’ve ever picked up a bottle of CBD oil and read the label, you may have noticed Terpenes listed as an ingredient.
In a report by the National Highway Traffic Administration, it was found that an alcohol-impaired driver was involved in nearly one-third of all traffic crash fatalities. Impaired driving can result in several various penalties. Often times, the courts will order substance abuse evaluations, which can include a drug and alcohol urinalysis screening.
The United States is experiencing an outbreak of e-cigarette, or vaping, product use associated lung injuries (EVALI). In the latest November reports, over 2,000 cases of EVALI have been reported, and over 40 deaths have been confirmed, according to the CDC.
According to the EPA, PFAS are persistent in the environment and human body, and exposure to these compounds can lead to adverse health effects. Per/Polyfluoroalkyl substances (PFAS), also known as perfluorinated compounds (PFCs), can be found in food, commercial household products, the workplace, drinking water, and even in living organisms.
The impact of human mental disorders continues to burden the health and wellness of people worldwide. As these disorders become more and more recognized in modern-day living, the number of prescribed antipsychotics (APs) and antidepressants (ADs) continues to increase. According to the CDC, over a fifteen-year time span, the use of antidepressants has increased nearly 65%.
One in seven Americans say they use CBD products, according to a recent study by Gallup.
As the cannabis market grows, interest in more detailed analysis of cannabinoid profiles is expanding because more comprehensive data can be used for strain identification as well as to ensure more accurate potency testing.
Benchmark Scientific has been providing research laboratories with innovative, cost effective products and solutions designed to improve laboratory efficiency, safety and results.
Researchers use an Agilent HPLC and Poroshell Column to semi-automate synthetic opioid detection methods.
