Blog

29th Jan 2025

Revolutionizing UHPLC Connections: Introducing MarvelXACT by IDEX

Reliable HPLC fittings are essential for achieving accurate and consistent results in UHPLC (Ultra-High Performance Liquid Chromatography) applications. Improper fittings can result in leaks, l …

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29th Jan 2025

EHS Compliance: Safely Manage HPLC Solvent Waste

Many solvents and even samples used in high-performance liquid chromatography (HPLC) can be hazardous and/or flammable. As a result, it’s crucial to minimize health and safety risks to laboratory pers …

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29th Jan 2025

The 3 Most Common Applications for Headspace Vials

Gas chromatography (GC) is a commonly used method for separating volatile substances in mixtures. A particular technique, headspace GC, is used to isolate and examine specific volatile components with …

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28th Jan 2025

How Does Column Temperature Affect HPLC Resolution?

The temperature of an HPLC column impacts retention time, selectivity, peak shape, and pressure. Let’s explore each of these factors in more detail.Temperature Can Affect Retention Time Retention ref …

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28th Jan 2025

How to Save Money and Increase Accuracy on Sample Prep

Automation can quickly boost throughput and enhance sample preparation accuracy, but it’s typically more common in high-throughput labs. But what about tasks that are too small for full automation but …

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28th Jan 2025

Syringe Filters vs Filter Vials: What is the Best Method for Filtration?

For most chromatography applications, samples taken directly from the source typically need some form of preparation before they can be injected into the analytical instrument. Sample preparation can …

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28th Jan 2025

How To Choose the Right Vial for Chromatography

Modern liquid chromatography autosamplers typically accommodate 12 x 32mm vials, so we’ll assume that a 12 x 32mm vial will be compatible with your autosampler (note that Headspace autosamplers genera …

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28th Jan 2025

The Benefits of Glass Well Plate Inserts

96-well plates are commonly used consumables in high-throughput analytical labs that rely on mass spectrometry. Typically, polypropylene 96 deep well plates, available in 2mL square or 1mL r …

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28th Jan 2025

Syringe Filters for HPLC Sample Preparation: A Guide to Choosing the Right Filter for Consistent and Accurate Results

Sample preparation plays a key role in high-performance liquid chromatography (HPLC). Though the preparation process can vary depending on the application and the level of sample interference, filtrat …

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16th Jan 2025

How to Choose the Right Septa for Your Autosampler Vial Closure

When conducting LC and GC analysis, it’s essential to have a suitable container for storing and transporting your samples before injection. Chromatography vials are the most commonly used vessels, ava …

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16th Jan 2025

Maximizing Throughput in an HPLC Laboratory

Maximizing sample throughput in a laboratory offers numerous benefits, with cost savings being a key motivator. While optimizing HPLC analysis is highly advantageous, it's also important to explore ot …

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16th Jan 2025

What Should Forensic Labs Be Using for Blood Alcohol Testing?

Headspace injection combined with dual column GC-FID is a widely used technique for measuring blood alcohol concentration (BAC) in forensic laboratories. Given the serious implications of BAC test res …

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16th Jan 2025

How to Check for Leaks in a GC System

Maintaining a leak-free GC or GC/MS system is essential for obtaining reliable, reproducible, and accurate results. Even minor leaks can affect chromatography by reducing sensitivity, contaminating sa …

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16th Jan 2025

New and Dangerous Synthetic Opioids

Are New Synthetic Opioids Increasing Risks?Synthetic opioids are widely utilized in medical environments for their analgesic properties, offering relief to those experiencing severe pain. However, the …

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16th Jan 2025

Methods for Changing Peak Resolution in HPLC

Enhancing the resolution of closely eluting sample components presents a challenge that every chromatographer will encounter eventually. Various techniques for altering peak resolution in HPLC involve …

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10th Jan 2025

Why You Should be Using a Delay Column for PFAS Analysis

PFAS in the Environment TodayPoly- and perfluoroalkyl substances (PFAS) are becoming increasingly important to monitor as environmental contaminants worldwide. Often referred to as "forever chemicals, …

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10th Jan 2025

Which Type of Glass Vial is Best for your Application?

Glass autosampler vials are often chosen for their inertness, but with so many glass options available, how do you determine which type is best for your application? In this blog, we’ll explore the di …

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10th Jan 2025

How Does ICP-MS Work?

How Does an ICP-MS Work?Inductively coupled plasma mass spectrometry (ICP-MS) is a technique used to identify the elements present in a sample, unlike LC/MS and GC/MS, which measure molecules and comp …

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10th Jan 2025

Should You Use a Glass Pre-Filter Syringe Filter?

Should You be Using a Glass Pre-filter Syringe Filter?Effective sample filtration enhances the quality and consistency of analytical results, while also extending the lifespan of your instrument's rep …

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10th Jan 2025

What is the Smallest Sample Volume You Can Use in 96 Well Plates?

In high-throughput labs, 96-well plates provide numerous benefits compared to chromatography vials, such as cost efficiency and enhanced automation features. Various types of well plates are suited to …

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10th Jan 2025

What Sample Prep is Best for Vitamin D Analysis?

The increasing demand for more sensitive and specific analytical methods is driven by the need for low-level testing of endogenous vitamins and hormones in biological samples. A key example is the ris …

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9th Jan 2025

Safety Waste Compliance in One Easy Step

Improper waste collection and handling of hazardous materials can pose serious risks to both people and property in the lab. While the days of disposing of HPLC solvent waste down the drain are long g …

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9th Jan 2025

Three Reasons You’ll Want to Switch to Agilent Capillary Tubing

Stainless steel tubing is the preferred material for HPLC and UHPLC because it is compatible with a wide range of HPLC solvents and can handle very high pressures. PEEK tubing, on the other …

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9th Jan 2025

One Simple Step to Conserve Helium

As many in the GC industry know, the helium shortage remains a significant issue. A few weeks ago, we published a blog discussing alternative carrier gases. However, we understand that switching to hy …

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9th Jan 2025

Revisiting ‘The Big Pain’

During the pandemic, drug testing facilities were overwhelmed with COVID-19 testing, which caused other types of testing, such as drug screenings, to be deprioritized. As a result, routine employee dr …

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9th Jan 2025

What LC Column Should I Use for EtG and EtS Detection?

Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are metabolites produced by the liver following ethanol consumption. These substances can be identified in urine within 2-80 hours after alcohol intake. …

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9th Jan 2025

Using 96 Well Plates In High Throughput Laboratories

For high-throughput labs, using 96 well plates instead of autosampler vials offers considerable advantages, particularly in terms of automation. While not all steps in the process can be ful …

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9th Jan 2025

Do You Really Need Helium as Your Carrier Gas?

The unpredictable helium supply can pose challenges for chromatographers. Due to ongoing helium shortages, some laboratories are receiving only 50% of their usual supply. Chrom Tech offers alternative …

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6th Jan 2025

When to Use High Recovery Vials

There are two primary reasons labs use high recovery vials. The first, and perhaps most obvious, is when working with limited-volume samples. In these situations, retrieving the last few microliters i …

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6th Jan 2025

How Many Injections Can I Get on My HPLC Column?

It depends! Several factors influence how many injections you can perform on your column, including: Injecting "dirty" samplesAdhering to the recommended protocols and operating conditions provided by …

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6th Jan 2025

How do Stainless-Steel Fittings Differ from Polymer-Based Fittings?

Stainless-steel and polymer-based fittings are both commonly used in HPLC, each with its own set of advantages and disadvantages. The choice between them depends on the specific application and method …

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6th Jan 2025

Method Transfer between Poroshell and Zorbax HPLC Columns

One of the main advantages of using superficially porous particle (SPP) HPLC columns, compared to fully porous particles, is that they provide the efficiency of sub-2-µm fully porous columns without c …

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6th Jan 2025

True Crime and Chromatography

Mystery has always captivated people, and in recent years, true crime series—whether podcasts, TV shows, or books—have surged in popularity. These chilling, often gruesome tragedies have turned into a …

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6th Jan 2025

Reduce Instrument Downtime with Sample Preparation

Minimizing downtime in the laboratory not only saves valuable time but also reduces the costs associated with getting instruments back in operation. A simple way to reduce HPLC downtime is by extendin …

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20th Dec 2024

How to Connect a Syringe to HPLC Tubing

Connecting a syringe to your LC pump for tasks like priming the system (and other applications) is a common need. We've seen various inventive solutions in laboratories where people have connected the …

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20th Dec 2024

ASK BETHANY | Should I Use a Guard Column

HPLC ColumnsThe column is one of the most crucial components in an LC system, as it is where analytes are separated from a mixture for both qualitative and quantitative analysis. While HPLC colum …

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20th Dec 2024

ASK BEN | 4 Simple Steps to Find the Right GC Liner

We frequently receive questions about GC liners, particularly regarding which liner is best suited for a specific application. Selecting the right GC inlet liner is essential for proper sample analysi …

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20th Dec 2024

ASK BEN | How to Choose an HPLC or GC Syringe

Syringes play a crucial role in HPLC and GC systems. To analyze a sample mixture on an LC or GC column, the sample must be introduced into the flow path, and syringes make this possible. As a res …

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20th Dec 2024

What are the Benefits of Glass Vials in the Laboratory?

Chromatography vials are compact glass or plastic containers utilized in laboratories to hold and transport samples until they are ready for analysis by HPLC or GC. If you're reading this, it's likely …

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20th Dec 2024

An Essential Guide to HPLC Fittings for Your Laboratory

An Introduction to HPLC FittingsFittings are essential whenever tubing needs to be attached, adapted, or adjusted within the system, making secure connections crucial for successful chromatography. In …

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20th Dec 2024

Extra HPLC Pump Parts You Need To Have on Hand

HPLC pumps are designed to operate reliably 24/7/365. To ensure your pump stays in optimal condition, we recommend scheduling regular maintenance and keeping commonly replaced parts readily available …

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19th Dec 2024

4 Questions You Should Ask Before Buying an HPLC Pump

Chrom Tech's positive-displacement piston pumps are versatile tools used in a wide range of analytical, clinical, preparative, and fluid-metering applications. While many of our customers rely on our& …

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19th Dec 2024

How to Properly Swage HPLC Fittings

Being able to properly change and connect fittings on an HPLC system is an essential skill for any chromatographer. At some point, you'll need to adjust or tighten a connection within your HPLC setup. …

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19th Dec 2024

Detecting Synthetic Opioids With an Agilent HPLC and Poroshell Column

Opioid abuse remains a significant challenge in the medical field. Since 1999, unintentional overdose deaths involving opioid pain relievers have more than quadrupled, surpassing those related to …

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19th Dec 2024

Getting to the Bottom of Vial Inserts

If you have questions about chromatography vials, you've come to the right place. Vials are our specialty here at Chrom Tech, and we have several blogs dedicated to the topic. If you're unsu …

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19th Dec 2024

A Sneak Peak into the Largest HPLC Industry in the World - Biotechnology Applications

Biotechnology is a vast field and one of the largest industries globally, playing a crucial role in food and agriculture, medicine, genetic research, and beyond. The ability to analyze compounds and d …

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19th Dec 2024

Helpful Fluoropolymer Tubing Guide for Your HPLC Applications

What types of tubing are used in the laboratory?In liquid chromatography and other fluidic laboratory applications, there is a broad range of tubing materials, sizes, and specifications to choose from …

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19th Dec 2024

How Do You Dispose of HPLC Waste?

High-Performance Liquid Chromatography (HPLC) is a widely used technique across various industries for separating complex mixtures of known or unknown analytes into individual components. By utilizing …

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