Blog

27th Jun 2025

Essential HPLC Accessories for Liquid Chromatography

Key Highlights HPLC accessories play a crucial role in achieving optimal performance and accurate results. From fittings and tubing to filters and detectors, each accessory contributes to the effici …

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27th Jun 2025

Are Hydrogen Generators Safe?

While hydrogen gas cylinders are commonly used in laboratories, they pose various safety risks and can lead to unexpected instrument downtimes due to inconsistent delivery schedules. These problems c …

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27th Jun 2025

Pore Size vs. Particle Size in HPLC Columns

When selecting an  HPLC column, it is important to understand the different attributes and how they will affect your chromatography. Chemical properties, such as the type of surface of the stati …

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27th Jun 2025

The Benefits of Glass Well Plate Inserts

96-well plates are commonly used consumables in high-throughput analytical labs that rely on mass spectrometry. Typically, polypropylene 96 deep well plates, available in 2mL square or 1mL …

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27th Jun 2025

How To Regenerate a C18 HPLC Column

Typical Regeneration Procedure for Reversed Phase Columns: Regularly cleaning your column can help extend its lifespan, reducing the need for frequent replacements of HPLC Columns. For columns t …

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27th Jun 2025

Should I Use a Guard Column

HPLC Columns The column is one of the most crucial components in an LC system, as it is where analytes are separated from a mixture for both qualitative and quantitative analysis. While HPLC colu …

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27th Jun 2025

The Role of Solvent Gradients in HPLC Analysis and How ASI Static Mixers Can Improve Performance

High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used to separate, identify, and quantify components in a mixture. One of the key elements in HPLC analysis is the use …

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27th Jun 2025

How To Maximize HPLC Column Lifetime

All chromatographic columns experience wear over time. Key factors that contribute to the degradation of HPLC columns include sample injections, as sample matrices may contain residual proteins, lipid …

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27th Jun 2025

How Many Injections Can I Get on My HPLC Column?

It depends! Several factors influence how many injections you can perform on your column, including: Injecting "dirty" samples Adhering to the recommended protocols and operating conditions provided …

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27th Jun 2025

Measuring Gas Flow for Gas Chromatography

Maximizing Efficiency with the Agilent ADM Flow MeterDiscover how the Agilent ADM Flow Meter enhances lab efficiency by providing accurate, real-time measurements of dry gas flows for gas chromatogr …

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26th Jun 2025

HPLC Pump Care and Troubleshooting

Many of the technical support inquiries we receive at Chrom Tech are related to care and troubleshooting of our continuous process pumps. Some of the topics include:       …

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29th Jan 2025

Revolutionizing UHPLC Connections: Introducing MarvelXACT by IDEX

Reliable HPLC fittings are essential for achieving accurate and consistent results in UHPLC (Ultra-High Performance Liquid Chromatography) applications. Improper fittings can result in leaks, l …

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29th Jan 2025

EHS Compliance: Safely Manage HPLC Solvent Waste

Many solvents and even samples used in high-performance liquid chromatography (HPLC) can be hazardous and/or flammable. As a result, it’s crucial to minimize health and safety risks to laboratory pers …

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29th Jan 2025

The 3 Most Common Applications for Headspace Vials

Gas chromatography (GC) is a commonly used method for separating volatile substances in mixtures. A particular technique, headspace GC, is used to isolate and examine specific volatile components with …

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28th Jan 2025

How Does Column Temperature Affect HPLC Resolution?

The temperature of an HPLC column impacts retention time, selectivity, peak shape, and pressure. Let’s explore each of these factors in more detail.Temperature Can Affect Retention Time Retention ref …

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28th Jan 2025

How to Save Money and Increase Accuracy on Sample Prep

Automation can quickly boost throughput and enhance sample preparation accuracy, but it’s typically more common in high-throughput labs. But what about tasks that are too small for full automation but …

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28th Jan 2025

Syringe Filters vs Filter Vials: What is the Best Method for Filtration?

For most chromatography applications, samples taken directly from the source typically need some form of preparation before they can be injected into the analytical instrument. Sample preparation can …

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28th Jan 2025

How To Choose the Right Vial for Chromatography

Modern liquid chromatography autosamplers typically accommodate 12 x 32mm vials, so we’ll assume that a 12 x 32mm vial will be compatible with your autosampler (note that Headspace autosamplers genera …

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28th Jan 2025

Syringe Filters for HPLC Sample Preparation: A Guide to Choosing the Right Filter for Consistent and Accurate Results

Sample preparation plays a key role in high-performance liquid chromatography (HPLC). Though the preparation process can vary depending on the application and the level of sample interference, filtrat …

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16th Jan 2025

How to Choose the Right Septa for Your Autosampler Vial Closure

When conducting LC and GC analysis, it’s essential to have a suitable container for storing and transporting your samples before injection. Chromatography vials are the most commonly used vessels, ava …

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16th Jan 2025

Maximizing Throughput in an HPLC Laboratory

Maximizing sample throughput in a laboratory offers numerous benefits, with cost savings being a key motivator. While optimizing HPLC analysis is highly advantageous, it's also important to explore ot …

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16th Jan 2025

What Should Forensic Labs Be Using for Blood Alcohol Testing?

Headspace injection combined with dual column GC-FID is a widely used technique for measuring blood alcohol concentration (BAC) in forensic laboratories. Given the serious implications of BAC test res …

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16th Jan 2025

How to Check for Leaks in a GC System

Maintaining a leak-free GC or GC/MS system is essential for obtaining reliable, reproducible, and accurate results. Even minor leaks can affect chromatography by reducing sensitivity, contaminating sa …

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16th Jan 2025

New and Dangerous Synthetic Opioids

Are New Synthetic Opioids Increasing Risks?Synthetic opioids are widely utilized in medical environments for their analgesic properties, offering relief to those experiencing severe pain. However, the …

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16th Jan 2025

Methods for Changing Peak Resolution in HPLC

Enhancing the resolution of closely eluting sample components presents a challenge that every chromatographer will encounter eventually. Various techniques for altering peak resolution in HPLC involve …

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10th Jan 2025

Why You Should be Using a Delay Column for PFAS Analysis

PFAS in the Environment TodayPoly- and perfluoroalkyl substances (PFAS) are becoming increasingly important to monitor as environmental contaminants worldwide. Often referred to as "forever chemicals, …

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10th Jan 2025

Which Type of Glass Vial is Best for your Application?

Glass autosampler vials are often chosen for their inertness, but with so many glass options available, how do you determine which type is best for your application? In this blog, we’ll explore the di …

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10th Jan 2025

Should You Use a Glass Pre-Filter Syringe Filter?

Should You be Using a Glass Pre-filter Syringe Filter?Effective sample filtration enhances the quality and consistency of analytical results, while also extending the lifespan of your instrument's rep …

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10th Jan 2025

What is the Smallest Sample Volume You Can Use in 96 Well Plates?

In high-throughput labs, 96-well plates provide numerous benefits compared to chromatography vials, such as cost efficiency and enhanced automation features. Various types of well plates are suited to …

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10th Jan 2025

What Sample Prep is Best for Vitamin D Analysis?

The increasing demand for more sensitive and specific analytical methods is driven by the need for low-level testing of endogenous vitamins and hormones in biological samples. A key example is the ris …

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10th Jan 2025

How Does ICP-MS Work?

How Does an ICP-MS Work? Inductively coupled plasma mass spectrometry (ICP-MS) is a technique used to identify the elements present in a sample, unlike LC/MS and GC/MS, which measure molecules and com …

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9th Jan 2025

Safety Waste Compliance in One Easy Step

Improper waste collection and handling of hazardous materials can pose serious risks to both people and property in the lab. While the days of disposing of HPLC solvent waste down the drain are long g …

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9th Jan 2025

Three Reasons You’ll Want to Switch to Agilent Capillary Tubing

Stainless steel tubing is the preferred material for HPLC and UHPLC because it is compatible with a wide range of HPLC solvents and can handle very high pressures. PEEK tubing, on the other …

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9th Jan 2025

One Simple Step to Conserve Helium

As many in the GC industry know, the helium shortage remains a significant issue. A few weeks ago, we published a blog discussing alternative carrier gases. However, we understand that switching to hy …

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9th Jan 2025

Revisiting ‘The Big Pain’

During the pandemic, drug testing facilities were overwhelmed with COVID-19 testing, which caused other types of testing, such as drug screenings, to be deprioritized. As a result, routine employee dr …

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9th Jan 2025

What LC Column Should I Use for EtG and EtS Detection?

Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are metabolites produced by the liver following ethanol consumption. These substances can be identified in urine within 2-80 hours after alcohol intake. …

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9th Jan 2025

Using 96 Well Plates In High Throughput Laboratories

For high-throughput labs, using 96 well plates instead of autosampler vials offers considerable advantages, particularly in terms of automation. While not all steps in the process can be ful …

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9th Jan 2025

Do You Really Need Helium as Your Carrier Gas?

The unpredictable helium supply can pose challenges for chromatographers. Due to ongoing helium shortages, some laboratories are receiving only 50% of their usual supply. Chrom Tech offers alternative …

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6th Jan 2025

When to Use High Recovery Vials

There are two primary reasons labs use high recovery vials. The first, and perhaps most obvious, is when working with limited-volume samples. In these situations, retrieving the last few microliters i …

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6th Jan 2025

How do Stainless-Steel Fittings Differ from Polymer-Based Fittings?

Stainless-steel and polymer-based fittings are both commonly used in HPLC, each with its own set of advantages and disadvantages. The choice between them depends on the specific application and method …

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6th Jan 2025

Method Transfer between Poroshell and Zorbax HPLC Columns

One of the main advantages of using superficially porous particle (SPP) HPLC columns, compared to fully porous particles, is that they provide the efficiency of sub-2-µm fully porous columns without c …

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6th Jan 2025

True Crime and Chromatography

Mystery has always captivated people, and in recent years, true crime series—whether podcasts, TV shows, or books—have surged in popularity. These chilling, often gruesome tragedies have turned into a …

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6th Jan 2025

Reduce Instrument Downtime with Sample Preparation

Minimizing downtime in the laboratory not only saves valuable time but also reduces the costs associated with getting instruments back in operation. A simple way to reduce HPLC downtime is by extendin …

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20th Dec 2024

How to Connect a Syringe to HPLC Tubing

Connecting a syringe to your LC pump for tasks like priming the system (and other applications) is a common need. We've seen various inventive solutions in laboratories where people have connected the …

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20th Dec 2024

ASK BEN | 4 Simple Steps to Find the Right GC Liner

We frequently receive questions about GC liners, particularly regarding which liner is best suited for a specific application. Selecting the right GC inlet liner is essential for proper sample analysi …

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20th Dec 2024

ASK BEN | How to Choose an HPLC or GC Syringe

Syringes play a crucial role in HPLC and GC systems. To analyze a sample mixture on an LC or GC column, the sample must be introduced into the flow path, and syringes make this possible. As a res …

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20th Dec 2024

What are the Benefits of Glass Vials in the Laboratory?

Chromatography vials are compact glass or plastic containers utilized in laboratories to hold and transport samples until they are ready for analysis by HPLC or GC. If you're reading this, it's likely …

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20th Dec 2024

An Essential Guide to HPLC Fittings for Your Laboratory

An Introduction to HPLC FittingsFittings are essential whenever tubing needs to be attached, adapted, or adjusted within the system, making secure connections crucial for successful chromatography. In …

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